Labshake search
Citations for Thermo Fisher :
801 - 850 of 5824 citations for VAPB Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... and sub cloned in BamHI and SalI site of pCDNA3.1/myc-His(A) mammalian expression vector (Invitrogen, USA) to generate pCDNA3.1-PAX9-myc-His(A ...
-
bioRxiv - Biochemistry 2021Quote: ... the array was washed and probed with an anti-His primary antibody (Thermo Fisher, MA121315, 1:1000 dilution), after which anti-mouse HRP-conjugated secondary antibody (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2021Quote: ... PCR was carried out with gene-specific primers (Table 1) using Phusion Hi-Fidelity PCR Mastermix (Thermo Scientific).
-
bioRxiv - Immunology 2020Quote: The PCR products obtained by SGA were cloned into the commercial directional vector pcDNA3.1D/V5-His-TOPO according to the manufacturer’s instructions (Invitrogen). The correct pcDNA3.1-Env plasmid used to produce wild type pdsudoviruses were selected by sequencing ...
-
Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
-
bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
-
bioRxiv - Immunology 2022Quote: ... Wildtype and mutant coding sequences were transferred to the pEF1/V5-His vector (cat. no. V920; Life Technologies) for overexpression ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
-
bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... We diluted anti-stem Abs (starting at the lowest concentration, which yielded measurable HI) in C1q-DPBS (Gibco) (220 nM) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription for other transcripts was performed using the hi-capacity cDNA reverse transcription kit (Applied Biosystems #4368814) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with a pMT Puro His-PC-SNAP-Elp3 vector using ExpiFectamine™ Sf(A389l5, Gibco) using manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of chicken anti-His antibody (Invitrogen, PA1-9531) on ice for 20 min ...
-
bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
-
bioRxiv - Microbiology 2023Quote: ... TCID50) were calculated by IFA using HIS α NDV and Alexa FluorTM 488 α-rabbit secondary antibody (Invitrogen) in two independent experiments.
-
bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
-
bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
-
bioRxiv - Cell Biology 2024Quote: ... His-tagged: The lysate was mixed head-over-head with Ni-NTA agarose resin (Thermo Scientific™, USA) for 60 min at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human GDNF (Thermo Fisher), 10ng/mL recombinant human CNTF (Thermo Fisher)).
-
bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human BDNF (Thermo Fisher), 10ng/mL recombinant human GDNF (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human CNTF (Thermo Fisher)).
-
bioRxiv - Neuroscience 2021Quote: ... mouse anti-human aSyn (Life Technologies, #180215) at 1:200 dilution ...
-
bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-human CD133 (Thermo Fisher Scientific) or rabbit anti-human Vimentin (Proteintech Group ...
-
bioRxiv - Bioengineering 2019Quote: Human AD-MSCs were purchased from Invitrogen. Human AM-MSCs were isolated from via informed consent under an IRB approved protocol from an ethics committee according to previously published methods.[12] All MSCs were expanded under standard culture conditions (37°C with 5% CO2 ...
-
bioRxiv - Cancer Biology 2019Quote: Human-codon optimized DNA (GeneArt, Thermo Fisher) comprising the CD8α-chain signal sequence ...
-
bioRxiv - Cell Biology 2019Quote: ... coli-derived recombinant human insulin (Gibco, A11382IJ), 64 μg ml−1 L -ascorbic acid 2-phosphate trisodium salt (Wako ...
-
bioRxiv - Cell Biology 2019Quote: ... anti-human-647nm (Alexa Fluor, ThermoFisher Scientific), all at 1/400 ...
-
bioRxiv - Bioengineering 2019Quote: ... 20 ng/ml human EGF (Life Technologies), 1 U/ml human thrombin (EMD Millipore ...
-
bioRxiv - Immunology 2019Quote: ... and Human GAPDH endogenous control (Applied Biosystems). qPCR was run on the Applied Biosystems Step One Plus Real Time PCR System ...
-
bioRxiv - Genomics 2019Quote: ... normalized to human-GAPDH (Hs02786624_g1, Applied BioSystems).
-
bioRxiv - Cell Biology 2019Quote: ... and 10ng/ml human bFGF (Invitrogen, PHG0021) or in L-DMEM (Gibico ...
-
bioRxiv - Bioengineering 2019Quote: ... with 10% AB Human Serum (Thermo Fisher) and IL-7 ...
-
bioRxiv - Immunology 2021Quote: ... or goat anti-human IgG (Invitrogen #A18805), (all diluted 1:15 000 in 0.1% BSA-PBS) ...
-
bioRxiv - Cell Biology 2021Quote: Human Episomal iPSC Line (ThF) (ThermoFisher, #A18945) was maintained in mTeSR1 medium (Stem Cell Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the following primers (human) from ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: Human protein arrays (ProtoArrays V5.0, Life Technologies) kept at -20°C were equilibrated at 4 °C for 15 min and ...
-
bioRxiv - Bioengineering 2020Quote: ... Goat anti-human Fc-HRP conjugate (Invitrogen) was used at a 1:10,000 dilution in blocking buffer for 1 h at RT ...
-
bioRxiv - Bioengineering 2021Quote: ... mouse anti-human CD63 (Thermofisher MEM-259), rabbit anti-human TSG101 (Abcam ab125011) ...
-
bioRxiv - Bioengineering 2021Quote: ... on recombinant human vitronectin (50μg/ml; Gibco) coated plates ...
-
bioRxiv - Bioengineering 2020Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen), 0.178 mM Adenine (Sigma) ...