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Citations for Thermo Fisher :
801 - 850 of 7449 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
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bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
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bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into the pAc5.1-V5/His vector (Invitrogen). Immunoprecipitations between Bdbt-EGFP and Myc-DAnkrd49 used GFP rabbit serum (ab290 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pcDNA-V5/His (Invitrogen) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The pcDNA4-TO-myc/his B backbone vector (Invitrogen) was chosen for overexpression ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-His antibody (Invitrogen cat. # MA1-21315-1MG) was used at the same concentration to coat the plates ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... were cloned in pcDNA3.1(-) Myc/His vector (ThermoFisher Scientific). Ligated plasmids were transformed in chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
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bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
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bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
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bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... araC and the araBAD promoter from pBAD/His (Invitrogen), the tetracycline resistance gene from pACE2 (Geneva Biotech ...
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bioRxiv - Biochemistry 2024Quote: Supernatant was supplemented with His-Pur cobalt resin (ThermoFisher), imidazole to 10 mM ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Physiology 2020Quote: ... the anti-transferrin receptor mouse monoclonal antibody (TransR, 1:1000, Thermo Fisher Scientific), and the anti-glyceraldehyde 3-phosphate dehydrogenase mouse monoclonal antibody (GAPDH ...
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bioRxiv - Microbiology 2020Quote: ... Toll-like Receptor (TLR) 4 (Thermo Fisher Scientific, Waltham, MA, USA, 1:2000), TLR7 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... conditioned media enriched in individual receptors was prepared in Expi293F cells (Thermo Fisher), transiently transfected with receptors expressed as ectodomains fused to a human Fc (IgG1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane fraction’s protein content was normalized by using anti-transferrin receptor antibody (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... appropriate primary antibody receptor targeting1:2,000 mouse PSD-95 (cat # MA1-046,Thermofisher), 1:200 rabbit GluA1 (cat # AB1504 ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... N-methyl-D-aspartate receptor 1 (NMDAR1, rabbit, 1:1000, Invitrogen PA5-34599), N-methyl-D-aspartate receptor 2B (NMDAR2B ...
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bioRxiv - Immunology 2022Quote: ... Selection for the KIR-CD3ζ receptor was performed by increasing the puromycin (Invitrogen) concentration to 1.0 µg/ml over 3-4 weeks and then maintaining at 0.5 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... THP-1 receptor cells were stained with CellTrace Violet (Thermo Fisher Scientific #C34557) at a final concentration of 1 μM in PBS for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant LRRK2 protein was from ThermoFisher (#A15197). For pT73 Rab10 assay ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). The transfection of primary NHKs to generate HPV18+ keratinocytes was performed as described previously [86] ...
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bioRxiv - Molecular Biology 2022Quote: ... RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and Protoscript II reverse transcriptase (New England BioLabs ...