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Citations for Thermo Fisher :
801 - 850 of 6525 citations for Recombinant Human AKT3 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... Protein extracts were cleared by centrifugation and purified with Pierce™ GST Spin Purification Kit (ThermoFisher Scientific, #16106). Western analysis was performed using standard procedures and used antibodies and concentrations are presented in Table S2.
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bioRxiv - Microbiology 2021Quote: ... washed with phosphate buffered saline (PBS) and the resin digested with His6-GST-HRV3C protease (Thermo Fisher Scientific) 16-18 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of GST-fused bait were coupled to Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602), previously washed twice with equilibration buffer (Tris-HCl 125 mM ...
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bioRxiv - Cell Biology 2021Quote: ... flow-through and washes were processed for western blotting using mouse anti-GST (at 1/1000 dilution; Invitrogen), followed by goat anti-Mouse IgG-HRP antibodies as described in the western blotting section.
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bioRxiv - Biochemistry 2022Quote: ... With the following exceptions: the nitrocellulose-coated coverslips were incubated with protein or with anti-GST antibody (Invitrogen) for 4 min ...
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bioRxiv - Pathology 2021Quote: ... The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific) according to the manufacturer’s instructions to detect the recovered TaCam3-His levels ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 1hr with a 1:1000 dilution of GST-Tag antibody (MA4-004, Thermo Fisher) in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... GST-PLK-2 was expressed in insect Sf9 cells using the standard Bac-to-Bac system (Life Technologies) and then purified using glutathione Sepharose (GE Life Sciences).
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bioRxiv - Physiology 2022Quote: ... GST-HGK was removed from the reaction using 10 μL of Glutathione Sepharose beads (Thermo Fisher Scientific #45000285) and batched incubation at 4 °C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: Mice were intraperitoneally injected with 10µg of purified GST-SLAPTAG or GFP in aluminum hydroxide (Imject Alum ThermoFisher). Two and four weeks later ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Labelling of 647His6-GST-CENP-E was performed according to the manufacturer’s instructions with the AlexaFluor647 (A20173A, Invitrogen). Degree of labelling estimated as 1:3.
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... with RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen cat# 10777-019).
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bioRxiv - Cell Biology 2021Quote: ... RNaseOUT recombinant ribonuclease inhibitor (200 U/mL; Thermo Fisher Scientific) and IGEPAL® CA-630 (0.5 % ...
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bioRxiv - Immunology 2021Quote: ... and recombinant epidermal growth factor (EGF, 2 ng/ml, Gibco).
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bioRxiv - Biochemistry 2020Quote: ... 40 U RNase inhibitor (RNaseOUT ™ Recombinant Ribonuclease Inhibitor, Invitrogen), and various amount of DNA sample (see figures) ...
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bioRxiv - Biophysics 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Neuroscience 2019Quote: ... 200 units/ml RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; Thermofisher), and 1X cOmplete™ ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins or commercially available RNase T1 (Thermo Fisher Scientific) were mixed with 0.5 pmol substrate RNAs in 10 µl RNase reaction buffer with EDTA (20 mM Tris-HCl pH7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and 40 U/μL of Recombinant Ribonuclease Inhibitor RNaseOUT (Invitrogen) at 25°C for 2 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin reagent (Life Technologies). After a three-day incubation period ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant monoclonal cystatin A antibodies (Thermo Fisher Scientific, MA5-29200) were immobilized on the silica surface of the microtoroid through EDC/sulfo-NHS covalent coupling ...
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bioRxiv - Cell Biology 2022Quote: ... Concentrations of recombinant proteins were measured by NanoDrop OneC (ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were prepared by Expi293 Expression System (Thermo Fisher) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... we created recombinant pCRTM4-TopoTM plasmids (Invitrogen, Carlsbad, CA, USA) containing 18S and 28S ribosome RNA PCR amplicons ...
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bioRxiv - Biochemistry 2022Quote: RNAseOUT™ recombinant ribonuclease inhibitor (ThermoFisher Scientific, cat. no. 10777019)
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bioRxiv - Genetics 2020Quote: ... 40 μL of recombinant protein G agarose beads (Thermo Fisher) were washed three times with 1 mL of ChIP dilution buffer by resuspending beads in the buffer ...
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bioRxiv - Microbiology 2020Quote: ... AcMNPV recombinant bacmids were constructed with the bMON14272 bacmid (Invitrogen) and maintained in Escherichia coli strain DH10B (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 μL of RNaseOut™ Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant SRPK1 and PKR were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant clones were selected with 120 μg/ml Hygromycin (Invitrogen) and pooled to obtain a stable population maintained in medium containing Puromycin ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... 200 U/mL RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher, 10777019), 400U/mL RNasin (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/mL recombinant bFGF (Thermo Fisher Scientific, PHG0261). Human iPSCs were converted to EPS cells referred to protocol described previously16 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Biophysics 2020Quote: The recombinant tubulin was expressed in HighFive cells (Life Technologies) and purified by three steps of column chromatography (DEAE Sepharose ion exchange chromatography ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated with mouse recombinant IFN-γ (BMS326, Invitrogen) at 6.25ng/mL for overnight and followed by infection with Mycobacterium tuberculosis ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated with recombinant IL-13 (PMC0134, Thermo Scientific) at 20ng/mL and IL-4 (PMC0045 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with recombinant murine EGF (100 ng ml−1, Gibco), recombinant human R-spondin 1 (200 ng ml−1 ...
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bioRxiv - Immunology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific, PMG8041), 1 ug/ml recombinant human R-spondin1 (R&D ...
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bioRxiv - Neuroscience 2022Quote: ... AlexaFluor™ 555 cholera toxin subunit B (recombinant) (Invitrogen #C22843)] of 1mg/mL (Molecular Probes® ...
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™ ...