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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were estimated using the BCA protein estimation kit (ThermoFisher) and subsequently separated by 10% SDS-PAGE and transferred onto nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were measured by BCA protein assay kit (ThermoFisher), equal amounts of proteins were analyzed for western blotting ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... quantified for protein using an EZQ Protein Quantification kit (Invitrogen), and separated on an 8% SDS-PAGE gel ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration was determined by Qubit Protein Assay Kit (Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration was determined by BCA Protein Assay kit (ThermoFisher). 10μg/sample with 4x Laemmli Buffer (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were determined using Qubit Protein Assay Kit (Invitrogen). We used 30 μg protein ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were quantified using EZQ protein assay kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... After protein quantification with BCA protein assay kit (23225, ThermoFisher), samples were mixed with SDS gel loading buffer (L-4390 ...
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bioRxiv - Biophysics 2021Quote: ... the microfluidic channel was incubated with 0.8 μM streptavidin (Invitrogen) in T50 buffer (50 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... the fusion proteins were diluted in mouse serum (Invitrogen) to 200 nM and incubated at 37°C for up to 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... or control mouse IgG and protein G Dynabeads (Invitrogen). Immunocomplexes were eluted by denaturation in NuPAGE LDS sample buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... multielectrode recordings were made with a silicon probe (P or E-style 16 channel probes, Cambridge NeuroTech) dipped in Di-I (Vybrant Multicolour Cell Labelling Kit, Thermofisher) while the mouse was head restrained over a freely rotating wheel ...
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bioRxiv - Biochemistry 2021Quote: ... Individual reactions contained 2 μM protein and 5X SYPRO Orange Protein Gel Stain (Invitrogen). DP7/maltoheptaose (Elicityl) ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-mouse VE-cadherin (VC) antibody was biotinylated in-house using FluoReporter Mini-Biotin-XX Protein labeling kit (Invitrogen). Cell populations were sorted using a FACSAria-II sorter (BD ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the specific protein signal was achieved by using WesternBreeze Chemiluminescent (anti-rabbit or anti-mouse) kit (Invitrogen) or by using Immobilon Forte Western HRP substrate (Merck)
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...
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bioRxiv - Neuroscience 2020Quote: ... whole brain lysates were prepared as for ELISA and protein concentration determined using a BCA assay (Thermo Fisher). Wells were loaded with 30 µg of total protein which was resolved by SDS-PAGE under reducing conditions and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2023Quote: ... 2ug/mL of SARS CoV2 BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... peptides were separated on a separation column (Acclaim PepMap100 C18, 75 mm i.D., 2 mm particle size, 100 Å pore size; Thermo Scientific) with a length of 50 cm ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were then separated on a separation column (Acclaim PepMap100 C18, 75 mm i.D., 2 mm particle size, 100 Å pore size; Thermo Scientific) with a length of 50 cm ...
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bioRxiv - Cell Biology 2019Quote: ... peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap × 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm × 50 cm ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were dotted (2 µL per dot) on the nitrocellulose membrane of pore size of 0.45 µm (Thermo Fisher Scientific, USA), and the membrane was dried at 30 °C for 15 min ...
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bioRxiv - Biochemistry 2019Quote: C18 LC-MS/MS used an Acclaim PepMap 100 C18 nanocolumn (0.075 mm × 150 mm, 2 μm particle size, 100Å pore size, Thermo Fisher Scientific) coupled to a 300 μm i.d ...
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bioRxiv - Plant Biology 2022Quote: ... the analyte was transferred to the analytical column (AcclaimR PepMap RSLC column, 50 μm i.d. × 15 cm, C18, 2 μm particle size, 100 Å pore size; Thermo Scientific) and separated at 60°C using a 90 min gradient from 5% to 95% solvent B at a flow rate of 220 nL/min (solvent A ...
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bioRxiv - Plant Biology 2022Quote: ... peptides were pre-concentrated with 0.1% TFA on a trap column (AcclaimR PepMap 100 column, 100 μm i.d. × 2 cm, C18, 5 μm particle size, 100 Å pore size; Thermo Scientific) at a flow rate of 7 μL/min for 10 min ...
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bioRxiv - Biophysics 2024Quote: ... Peptides reconstituted in 0.1% formic acid were loaded onto a trap column (Acclaim C18 PepMap 100 nano Trap, 2 cm × 100 μm I.D., 5-μm particle size and 300-Å pore size; Thermo-Fisher Scientific) at 15 μL/min for 3 min before switching the precolumn in line with the analytical column (Acclaim C18 PepMap RSLC nanocolumn ...
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bioRxiv - Neuroscience 2022Quote: ... media were removed and cells were rinsed in DPBS without calcium and magnesium before adding 2 mL of pre-warmed StemPRO Accutase (Gibco®, A11105-01) to each flask ...
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bioRxiv - Cell Biology 2023Quote: ... using an automated simultaneous pipettor (analytikjena CyBio® Well vario) and incubated with the fluorescent calcium indicator Fluo4 (2 µM, Life Technologies, #F14202) in DMEM without supplementation for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Two days after transduction cells were selected with Puromycin (2 µg/mL, Thermo Fisher Scientific, Waltham, MA) for at least 1 week ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μg/ml Alexa Fluor 633-conjugated goat anti-Rabbit IgG for two hours (Thermo Fisher Scientific Cat# A-21071 ...
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bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
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bioRxiv - Immunology 2022Quote: ... Milk was diluted two times in D-PBS with 2% (v/v) of horse normal serum (Gibco) and centrifuged at 1,000g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured at high density and split every two days using 1-2 min TrypLE (Gibco) incubation ...
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bioRxiv - Cell Biology 2023Quote: Two days before differentiation (Day -2) hiPSCs were enzymatically dissociated as single cells using TryplE Select (Gibco), plated on the specific culture device in StemMACS iPS-Brew XF (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: Transient knockdown of NaV1.7 mRNA was done using short interfering RNA (siRNA) targeting both mouse and rat channel transcripts (Cat# 4390771, ID: s134909; Thermo Fisher Scientific Ambion, Waltham, MA, USA). Permanent knockout of endogenous NaV1.7 in ND7/23 cells was achieved using CRISPR/Cas9 genome editing with plasmid pD1301-AD (pCMV-Cas9-2A-GFP ...
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bioRxiv - Cell Biology 2023Quote: ... antibody concentration (ng/ml) in collected media was determined with Total Mouse IgG enzyme-linked immunoassay (ELISA, Invitrogen) and extrapolated from generated standard curves ...
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bioRxiv - Biochemistry 2020Quote: ... transferred to nitrocellulose membranes (0.45 µM pore, Invitrogen) and probed with antibodies in PBST buffer (PBS containing 0.05% Tween 20 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells grown in 24-well plates were transfected with pGFP-GCK (0.5 μg/well) using a calcium phosphate kit (Invitrogen Inc.). The next day ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids were transfected into Drosophila S2 cells using Calcium Phosphate Transfection Kit (Thermo Fisher Scientific, Paisley, Scotland, UK). Following antibiotic selection with Zeocin (InvivoGen ...
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bioRxiv - Cell Biology 2022Quote: ... and transfected with pMT-GAL4 (Drosophila Genomics Resource Center) and pUAST plasmids containing YN and YC fusions using the calcium phosphate transfection kit (Invitrogen). 10 mg of each construct were used ...
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bioRxiv - Neuroscience 2020Quote: hiPSC-derived neuron and astrocyte co-cultures were loaded with Fluo 4 direct (calcium assay kit by Thermo Fisher Scientific) for 30 minutes in 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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bioRxiv - Neuroscience 2023Quote: ... and loaded with the Fluorescent Dye-Based Rhod-3 AM following the manufactures’ instructions (Rhod-3 Calcium Imaging Kit, Cat.No. R10145; ThermoFisher scientific). Loading ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...