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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Mouse ANKRA2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Gapdh (mouse) (Life technologies) (24) ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse (Thermo Scientific) or by HRP-conjugated secondaries (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... Laminin Mouse Protein (ThermoFisher) was added to the solution containing calcium and cells to a final concentration of 1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... anti-Mouse IgG (Invitrogen) was used as isotype control ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse (A11017; Invitrogen), Alexa Fluor 488 goat ...
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bioRxiv - Biochemistry 2019Quote: ... mouse IgG (ThermoFisher Scientific), goat IgG (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Myc (Thermo Fisher, mouse). The following day ...
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bioRxiv - Plant Biology 2020Quote: ... anti-mouse Alexa488 (Invitrogen) and anti-rabbit CY3 (Dianova)-conjugated secondary antibodies were diluted 1:600 ...
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bioRxiv - Immunology 2020Quote: ... then anti-mouse (Invitrogen) or anti-human (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... mouse V5 (Invitrogen V8012), sheep Scp105R (from Dr ...
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse IgG2a (ThermoFisher)] diluted 1:1000 for 1hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Mouse α-GAPDH (ThermoFisher) was used at 1:10,000 final dilution ...
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bioRxiv - Physiology 2022Quote: ... and mouse (Invitrogen, A11004) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-GAPDH (Invitrogen)] ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse laminin (Invitrogen) coated 12 mm glass coverslips (Warner Instruments ...
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bioRxiv - Physiology 2023Quote: ... SERCA1a (Thermofisher, ma3912 mouse), RyR (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-H3K4me3 (Invitrogen). Secondary antibodies were purchased from Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse depletion Dynabeads (Invitrogen) were used for the depletion of lineage-labeled cells using a DynaMag-15 magnet (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... and mouse serum (Invitrogen). RQR8+CD4+ and RQR8+CD4- CAR-T cells were FACS-sorted with a Sony SH800S cell sorter into FCS ...
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bioRxiv - Bioengineering 2024Quote: ... and mouse serum (Invitrogen). B7H3- CD34+CD4+ and B7H3- CD34+CD4- CAR-T cells were FACS-sorted with the Sony SH800S cell sorter into FCS ...
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bioRxiv - Cell Biology 2023Quote: ... mouse IgG (Invitrogen, 10400C), goat anti-rabbit HRP-conjugated IgG (Pierce ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-GFP (Invitrogen) 1/50 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse-IgG (Fisher Scientific), antibodies against HA tag (Biolegend) ...
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bioRxiv - Molecular Biology 2022Quote: Resting primary B and T cells were isolated from mouse spleens by negative selection using Mouse CD43 (Untouched™ B Cells) and Untouched Mouse T Cells Dynabeads respectively (Invitrogen). For experiments done in quiescent splenocytes ...
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bioRxiv - Microbiology 2019Quote: ... goat-α-mouse-Alexa-488 and goat-α-mouse-Alexa-594 (Life Technologies). FISH was performed using a PNA probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy5 ...
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bioRxiv - Systems Biology 2024Quote: ... Incubations were performed with mouse anti-FLAG or mouse anti-V5 (ThermoFisher Scientific), and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and treated with 1x ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used were against mouse (Donkey anti-mouse, AF488; Invitrogen Cat#A21202) or goat (Donkey anti-goat AF488 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics (Fig ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Large quantities of purified plasmids containing AsegIRs and AsegIR8a with verified sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of AsegIRs and AsegIR8a were synthesized from NotI (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106/ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV as a junk plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µg of lentiviral plasmid packaging mix (24) and 5 µg of pooled sgRNA library plasmid was transfected using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-transfected with 1 μg of the corresponding plasmids and 10 ng of a Cre recombinase plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Stable cell lines were selected after 2 days of growth by addition of 1 μg ml−1 puromycin ...
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bioRxiv - Immunology 2019Quote: ... The sgRNA was cloned pCas-Puro-U6 plasmid and the pCas-Puro-U6 plasmid Linear was obtained using the BbsI restriction enzyme (Thermo Fisher Scientific). The plasmids containing sgRNA were transfected into IPEC-J2 cells with GeneTran III (Biomiga ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells in 6-well plates were transfected with 1.0 μg pMJ920 plasmids and 1.0 μg gRNA expression plasmids by Lipofectamine® 2000 Transfection Reagent (Invitrogen, Cat.11668-027) using the recommended protocols ...
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bioRxiv - Genomics 2020Quote: ... Correct assembly of the sgRNA into the backbone was confirmed by SANGER-Sequencing (Microsynth) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 μg FLAG-hRpn13-expressing plasmid or 5 μg FLAG-hRpn131-279-expressing plasmid by using lipofectamine 3000 (Thermo Fisher Scientific, L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNA expressing plasmids were produced by cloning sgRNA oligonucleotides with the appropriate adaptor sequences (see Table S2) into plasmid pMCB306 after digesting the plasmid with restriction enzymes BstXI and Bpu1120I (Cat# FD1024, FD0094, Thermo Fisher Scientific). pMCB306 was a gift from Michael Bassik (Addgene plasmid #89360 ...
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bioRxiv - Plant Biology 2021Quote: ... Positive colonies were grown in LB overnight and then used for subsequent plasmid isolation (GeneJET Plasmid Miniprep Kit; Thermo Fisher Scientific, USA). About 150 pUC18R6KT-mini-Tn7T-Km recombinant plasmids were stored at −4°C ...
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bioRxiv - Microbiology 2021Quote: ... The resulting sub-cloning plasmids and the conjugative plasmid pVZ321 were cleaved with restriction enzymes XhoI and SalI (Thermo Scientific™, Germany), generating compatible restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Large quantities of purified plasmids containing ItypORs and ItypOrco with correct sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of ItypORs and ItypOrco were synthesized from NotI (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 100 million HEK293T cells were seeded into a 15 cm2 plate with complete DMEM and transfected with 5 μg of lentiviral plasmid packaging mix and 5 μg of pooled sgRNA library plasmid using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded at 1×105 cells per well in a 24 well multiplate and transfected with 800 ng plasmid DNA per well (400 ng of SaCas9 expression plasmid and 200 ng of each sgRNA plasmid) using Lipofectamine 2000 (Thermo Fisher Scientific) as according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated overnight at 37°C in shaker and the plasmid DNA was extracted using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Lithuania). As the final means of verification sequence of inserted fragment was verified through Sanger Sequencing employing M13-Fw and M13-Rs primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each plasmid DNA (two plasmids expressing Cas9 and gRNAs) were mixed in 100 µl of reduced-serum medium OPTI-MEM (ThermoFisher Scientific, 31985062) then mixed with 15 µl of 1 mg/ml PEI ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...