Labshake search
Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Kininogen 1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
-
bioRxiv - Neuroscience 2020Quote: ... goat anti-human (ThermoFisher, 1:1000). Hoechst staining (ThermoFisher ...
-
bioRxiv - Genomics 2022Quote: ... or human cot-1 (15279011, ThermoFisher), and 0.6 μl of 100 μM TIME-Seq hybridization blocking primers (IDT) ...
-
bioRxiv - Microbiology 2022Quote: ... 1:2,000 goat anti-human (Invitrogen), 1:1,000 goat anti-mouse (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... Expi293 cells were transfected with the expression plasmid hACE2-1-740aa-8xHis or hACE2-1-614aa-8xHis using ExpiFectamine 293 (ThermoFisher Scientific). Four days later ...
-
Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... elegans BUB-1 TPR (1-189)::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
-
bioRxiv - Cell Biology 2022Quote: ... doxycycline-inducible GFP-EMC2 Flp-In 293 T-Rex cell line and its adaptation to suspension growth in FreeStyle 293 Expression Medium (Thermo Fisher Scientific, USA) was described before (Pleiner et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
-
bioRxiv - Physiology 2024Quote: ... HEK 293T cells were transiently transfected with indicated constructs using the lipo2000 (1 mg/ml, LIFE technologies) reagents ...
-
bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
-
bioRxiv - Molecular Biology 2020Quote: ... MSC and HEK cell-lines were maintained in high-glucose (4.5 g/l) DMEM GlutaMax (Thermo Fisher Scientific, Waltham, MA, USA; HEK) or RPMI medium 1640 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... the cells were washed and cultured in the presence of hygromycin to select DC-SIGN expressing HEK-293T cells (DC HEK) (Thermo Fisher Scientific). Similarly ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK 293T cells were transfected using Lipofectamine 2000 (ThermoFisher), as per manufacturer’s instructions and standard procedures ...
-
Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher) in a T-25 flask.
-
bioRxiv - Cancer Biology 2020Quote: HEK 293T cells were cultured in DMEM media (GIBCO) supplemented with 10% FBS (GIBCO) ...
-
bioRxiv - Bioengineering 2020Quote: HEK 293T cells were cultured in DEME (Gibco, 11995115) with 10% FBS (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: ... HEK-293T cells were transfected using Lipofectamine 2000 (Invitrogen) for lentiviral expression vector pLL5.0 and third generation packaging constructs pMDLg/pRRE ...
-
bioRxiv - Microbiology 2021Quote: ... HEK-293FT cells (R70007) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... HEK-293A cells (R705-07) were purchased from Invitrogen. Murine 10.1 fibroblasts have been described [61] ...
-
bioRxiv - Bioengineering 2022Quote: HEK 293T cells were cultured in DMEM (11885, Gibco) supplemented with 10% FBS (Gibco 10082147 ...
-
bioRxiv - Cell Biology 2021Quote: ... Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher) in a T-25 flask.
-
bioRxiv - Synthetic Biology 2020Quote: ... HEK-293T cells were obtained from Invitrogen (Carlsbad, CA) as Lenti-X 293 cells for lentivirus production ...
-
bioRxiv - Cell Biology 2019Quote: ... HEK-293t cells grown in 6-well plates (Nunc) were transfected with biosensor DNA plus regulator if required ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were transfected into HEK 293FT packaging cells (Invitrogen) using Lenti-X high-titer lentiviral packaging systems (Clontech) ...
-
bioRxiv - Developmental Biology 2022Quote: ... were co-transfected into HEK-293FT cells (Invitrogen R70007), and after 48 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... 1x105 HEK cells were resuspended in media (DMEM, Gibco) with penicillin/streptomycin (0.5× ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK-293FT (humane embryonic kidney, Invitrogen no. R700-07) cells were cultured the same way ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK-293T cells were transfected using Lipofectamine 3000 (Invitrogen) with 500ng of the psPAX2 ...
-
bioRxiv - Biophysics 2021Quote: ... The recombinant constructs of heavy and light chain were transfected at 1:1 ratio into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (Gibco™ ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant antibodies were expressed in Expi293 cells following co-transfection of heavy and light chain plasmids (1:1 ratio) using Expifectamine 293 (Thermo Fisher Scientific). Supernatants were harvested after 5-6 days ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
-
bioRxiv - Immunology 2022Quote: ... incubated with DPBS containing 10% HI FBS and 1:1000 Hoechst 33342 (Invitrogen, #H3570) for nuclear staining and mounted onto microscope cover slips with Fluoromount G (Southern Biotech ...
-
bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
-
bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
-
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: Flp-In™ T-REx™ 293 cell line (Invitrogen) containing a single stably integrated FRT site and expressing Tet repressor were used for generation of inducible cell lines ...
-
bioRxiv - Molecular Biology 2021Quote: Flp-In™ T-REx™ 293 cells (ThermoFisher, R78007) were cultured at 37°C and 5% CO2 in DMEM media with 10% FBS and 1% penicillin-streptomycin and split every 2-3 days before reaching full confluence ...
-
bioRxiv - Cell Biology 2022Quote: ... were transfected into 293 package cells with Lipofectamine 2000 (Invitrogen) under DMEM (10% FCS ...
-
bioRxiv - Developmental Biology 2019Quote: HEK293 (Flp-In™-293 Cell Line, Thermo Fisher Scientific) were grown in DMEM supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2020Quote: ... GripTite™ MSR 293 cells (Invitrogen cat no. R795-07) were cultured as per manufacturer’s instructions and were seeded at 3×104 cells per well in a 96 well plate the day prior to starting the assay (24hrs +/- 2 hrs) ...
-
bioRxiv - Biochemistry 2021Quote: ... Pure DNA was transfected using ExpiFectamine 293 Transfection Kit (ThermoFisher) using the manufacturer protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Transfection was performed using ExpiFectamine 293 reagent (Thermo Fisher Scientific). Cell culture supernatant was collected seven days after transfection ...
-
bioRxiv - Microbiology 2020Quote: ... Transfection was performed using ExpiFectamine 293 reagent (Thermo Fisher Scientific). Cell culture supernatant was collected three days after transfection and supplemented with 10x PBS to a final concentration of 2.5x PBS (342.5 mM NaCl ...
-
bioRxiv - Immunology 2021Quote: ... was transiently expressed in FreeStyle 293 expression system (Thermo Fisher). SIRPα-hIg was then purified with Sepharose Protein A/G beads ...
-
bioRxiv - Molecular Biology 2021Quote: ... 293 cells were transfected using Lipofectamine 2000 (11668030, ThermoFisher Scientific) with plasmid YTHDC1-FLAG and cell lysates were generated as previously described(Meredith et al. ...
-
bioRxiv - Microbiology 2020Quote: ... and maintained in FreeStyle 293 serum-free medium (Thermo Fisher). Two days after transfection approximately 5 × 105 cells were transferred to 96-well plates ...
-
bioRxiv - Immunology 2022Quote: ... were transiently transfected in FreeStyle 293-F cells (Thermo Fisher) using Turbo293 (SpeedBiosystems ...
-
bioRxiv - Immunology 2020Quote: ... using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific Inc.). Seven days post transfection ...
-
bioRxiv - Molecular Biology 2020Quote: ... 80 μg DNA and 216 μl Expifectamine 293 (Thermo Fisher) were prepared in 4 ml OptiMEM ...
-
bioRxiv - Immunology 2022Quote: ... were transfected into GP2-293 cells using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... were cultured in Freestyle 293 media (Thermo Fisher, cat#12338026). Vero cells (ATCC CCL-81) ...