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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with an expression plasmid encoding human ACE2 - C-terminally fused to the GFP - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... falciparum cell lines were cultured in vitro in human erythrocytes (UK Blood and Transfusion Service) at 37°C in RPMI-1640 (Gibco) based media supplemented with 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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bioRxiv - Immunology 2021Quote: ... The percentage of infected cells (p24+) was determined by gating the living cell population on the basis of viability dye staining (Aquavivid; Thermo Fisher Scientific). Samples were acquired on an LSR II cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... The percentage of infected cells (p24+) was determined by gating the living cell population on the basis of viability dye staining (Aquavivid; Thermo Fisher Scientific). Samples were acquired on an LSR II cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... The percentage of infected cells (p24+) was determined by gating on the living cell population according to a viability dye staining (Aqua Vivid; Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were centrifuged for 15 minutes at 13 000 g at 4°C and the supernatant was stored at -80°C before analysis for total protein concentrations using the Bradford protein assay (Thermo Fisher Scientific, Leicestershire, UK, 23200). Protein in each sample was quantified from a standard curve using BSA standards (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lysates were centrifuged at 15,000 g for 10 min at 4 °C and the supernatant was quantified for total protein concentration using the Pierce BCA Protein Assay (Thermo Fisher Scientific, Cat. No. 23225). Thirty micrograms of protein were loaded onto sodium dodecyl sulfate−polyacrylamide gel for electrophoresis (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-chromatin complexes were isolated using a 1:1 mixture of magnetic Protein A- and Protein G-dynabeads (ThermoFisher Scientific) and washed three times with a solution of 50 mM HEPES-KOH ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Soluble chromatin supernatant equivalent to 6 μg per IP was pre-cleared using 20 μl of a 1:1 mix of protein A and protein G Dynabeads (Invitrogen). Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... 1% of input chromatin was saved and 70 μg of chromatin pre-clearing was done by mixing with pre-blocked Sepharose beads (1:1 vol. mix of Protein A and protein G beads; Invitrogen) for 1h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... the phage:antibody complexes were immunoprecipitated using 40 μL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) (4°C for 4 hours ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The virus samples were treated with 300 units of RNase I (Ambion) for 30 min followed by SUPERaseI RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mL virus with 10μg/mL polybrene (Thermo Fisher Scientific TR-1003-G) was added to 5×105 cells plated in 6-well dishes ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... with 40 cycles of 15 s at 95°C and 1 min at 61°C (Applied Biosystems 7500). Primers used in this study are listed in Table S2.
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bioRxiv - Molecular Biology 2021Quote: ... and digested at 37°C for three hours using 1:200 w:w endoproteinase Lys-C (Thermo Fisher Scientific). The urea was then diluted to 1.5 M and samples further digested at 37°C overnight with 1:25 w:w trypsin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using TaqMan Fast Virus 1-Step Master Mix (Thermofisher Scientific, US) with primers and probe specific for the SARS-CoV-2 E gene following guidelines by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assayv1991527e5122341d99287a1b17c111902.pdf ...
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bioRxiv - Microbiology 2021Quote: ... HCV RNA was quantified by one-step reverse transcription-quantitative PCR using 50 ng of total intracellular RNA and TaqMan® Fast Virus 1-Step Master Mix (Applied Biosystems) with primers and probe targeting the HCV 5’ nontranslated region as described previously [82] ...
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bioRxiv - Microbiology 2022Quote: The ZEBOV RT-PCR assay was performed in a 25 µL reaction mixture containing TaqMan Fast Virus 1-step master mix (Thermo Fisher Scientific), 5 µl template RNA ...
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bioRxiv - Microbiology 2021Quote: ... RNA extracted from animal samples or RNA standards were then measured using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Genetics 2022Quote: ... 30 μg of the virus) was digested with 1:100 (w/w) ratios of trypsin (Pierce Trypsin Protease mass spectrometry grade; Thermo Fisher Scientific)/virus for various time points at 25°C (31 ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) of the extracted RNA samples (1 µg) was performed using AMV (Avian Myeloblastosis Virus) Reverse Transcription Kit (Invitrogen, Waltham, USA) and oligo-dT18 (1 µM ...
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bioRxiv - Neuroscience 2023Quote: ... from the Stanford Gene Vector and Virus core unless otherwise specified) and Ctb retrograde tracers (1 ug/ul; Invitrogen #C34777, #C34776, #C34775) were infused with a syringe pump (Harvard Apparatus ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...
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bioRxiv - Immunology 2023Quote: ... The next day cells were infected with VSV-GFP virus at multiplicity of infection (MOI) of 1 in serum-free DMEM (DMEM Thermo Fisher, 11965092). After 1hr of incubation with media containing virus ...