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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... pH=7.4) of mice (1-3 months) using a vibratome (Microm HM 650V, Thermo scientific).Slices were then transferred to a slice chamber with ACSF (in mM ...
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bioRxiv - Immunology 2023Quote: ... 3 μm tissue sections were stained with polyclonal goat anti-mouse CD8α (1:2000, Invitrogen) antibody ...
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bioRxiv - Genomics 2023Quote: ... 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP) (dATP, Thermo Fisher R0141 ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the embryos were pre-cleared in 1% potassium hydroxide (KOH) (Fisher Scientific, 1310–58-3) solution for 1 hour and stained in 0.005% alizarin red (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070; Gibco).
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bioRxiv - Cell Biology 2022Quote: ... anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; catalog number MA5-15738; Invitrogen; dilution 1:5000), anti-TDP-43 (catalog number 10782-2-AP ...
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bioRxiv - Microbiology 2023Quote: ... using lipofectamine at the proportion 1:3 (DNA / Lipofectamine) in Opti-MEM (Thermo Fisher Scientific), and after 4 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used as primary antibodies: FFAR 3 (1: 100, #PA5-97745, Invitrogen) and GPR 43(1 ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were immunostained with anti-beta-3 Tubulin (Invitrogen, cat. no. 2G10-TB3, 1:500) in 2.5% NDS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 594-conjugated anti-mouse IgG (1:1000 in PBS/3% BSA (Invitrogen, A11032)) was used as secondary antibody and incubated for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... containing fungal cells at MOI 1:3 and 0.02 µg/µL propidium iodide (Fisher Scientific). Images were taken every 3 min for 2 hours on an Ultraview VoX spinning disk confocal microscope using Volocity software (Version 6.5.1).
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bioRxiv - Neuroscience 2024Quote: ... 3% normal donkey serum and donkey anti-rabbit Alexa Fluor 647 (1:400, ThermoFisher Scientific) in a total volume of 5 ml per sample ...
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bioRxiv - Microbiology 2024Quote: ... with specific primers and probes (Supplemental Table 1) and using a Quantstudio 3 (Applied Biosystems). The expressions of HCoV-229E M gene and SARS-CoV-2 E gene were quantified using a standard curve.
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bioRxiv - Bioengineering 2024Quote: ... at a DNA:PEI ratio of 1:3 (w/w) or Lipofectamine 2000 (Thermo Fisher Scientific) at a DNA:Lipofectamine ratio of 1:2 (w/v) ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 x DPBS+/+ and incubated in 1 μg/ml DAPI (Life Technologies) for 10 minutes at room temperature in DPBS+/+ ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with diluted trypsin solution (1:3, in 1X DPBS [Gibco, 14040-091]), and allowed to grow further or processed for experiments.
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bioRxiv - Immunology 2024Quote: ... were produced in HEK293F by transfecting with PEI (3:1 ratio) or Expi-293F (ThermoFisher) cells following the manufacturer’s recommended transfection protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and TO-PRO-3 was used to label cell nuclei (1 µM, Thermo Fisher Scientific). Images were acquired with a confocal microscope (Leica SP5 ...
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bioRxiv - Developmental Biology 2024Quote: ... cerebella were washed 3 x 1 h in 50% OCT (Fisher Scientific, 23-730-571) before embedding in 100% OCT ...
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bioRxiv - Genetics 2024Quote: Cell pellets (1 x 10^5 – 3 x 10^6) were washed with PBS (Gibco), resuspended in RLB (10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... were coated with 1 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) of anti-CD3 antibody (16-0032-81, Thermo Fisher) in PBS at 4 °C overnight ...
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bioRxiv - Genetics 2022Quote: ... and washed 3-4 times with fresh 10% RNA later solution (Thermo Fisher Scientific). After the last wash ...
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bioRxiv - Zoology 2022Quote: ... The excised tissues were then placed (3-4 pieces) on gelatin (Attachment Factor, ThermoFisher)-coated dish (35 mm ...
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bioRxiv - Molecular Biology 2021Quote: ... 3-4 μl of samples were applied onto grids using a Vitrobot (Thermo Fisher). Grids were blotted with “595” filter papers (Ted Pella ...
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bioRxiv - Genetics 2021Quote: Calu-3 cells were mock transfected with 4 μL of lipofectamine 3000 (ThermoFisher Scientific) in Opti-MEM (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was replaced after 3-4 hr with neurobasal medium (Gibco, Cat# 21103049) supplemented with 2% v/v B27 (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3 times with DMEM and fixed with 4% paraformaldehyde (Thermo Fisher) at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... with 10% FBS and split every 3-4 days using TrypLE Express (Thermo Scientific). Low-passage ...
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bioRxiv - Microbiology 2023Quote: ... sonicated via a Sonic Dismembrator 100 (Fisher Scientific, setting 3, 15 s, 4 pulses), and incubated on ice for 1 hr after the addition of 2 μL benzonase (EMD Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days using 0.25% Trypsin-EDTA (Fisher Scientific) incubated at 37°C for 3-5 minutes to detach cells ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 h at 4°C and then with protein G magnetic beads (ThermoFisher) at 4°C for an additional 2 h to obtain immunoprecipitated RNA fragments ...
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bioRxiv - Genomics 2024Quote: ... were treated 3-4 times with 50 U of T4 PNK (Thermo Fisher Scientific) and 100 U of λ-exonuclease (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... using a 10 min loading at 3 µL/min flow rate to a trap column (Acclaim™ PepMap™ 100, 2 cm × 75 µm, 3 µm, 100 Å - ThermoFisher Scientific). The separation was performed on an EASY-Spray™ C18 analytical column (25 cm × 75 µm ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA expression was analyzed based on 2-3 biological replicates each with 3 technical repeats using QuantStudio™ Design & Analysis Software (Thermo Fisher Scientific Inc.). The 18S-rRNA housekeeping gene was used as an internal reference gene ...
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bioRxiv - Biochemistry 2024Quote: ... washed 3x in PBS and subsequently for 2h with goat anti-rabbit 555 (1:1500) or donkey anti-sheep 647 (1:1500) respectively (ThermoFisher Scientific). Coverslips were mounted using Prolong gold with DAPI (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... with single-cell passaging every 2-3 days using accutase (ThermoFisher Scientific A1110501) at split ratios between 1:5 and 1:12.
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Neuroscience 2021Quote: ... TRPA1−/− male mice were loaded with Fura-2 (3 μg/mL, Life Technologies) and pluronic acid (1:1 ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... were all purchased from Sigma and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium was purchased from Thermofisher.
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bioRxiv - Biochemistry 2020Quote: ... and cells were loaded with 3 μM Fura-2 AM dye (Molecular Probes) in sterile-filtered HEPES-Tyrode Buffer (HTB ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... p97 siRNAs (2-HSS111263 and 3-HSS111264) were from Invitrogen (Thermo Fisher Scientific). p97 rescue constructs were previously published 53 and were resistant to siRNA # 2 ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 and Caco-2 cells were cultured in minimum essential medium (GIBCO) while A549-ACE2 cells were maintained in DMEM/F-12 medium (GIBCO) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) (M6494, Thermo Fisher Scientific) was added to each well to a final concentration of 0.67 mg/ml and incubated at 37oC ...