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Citations for Thermo Fisher :
801 - 850 of 1061 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... [18F]CHDI-961 was released with 0.5 ml of 96% ethanol via a Nalgene sterilising filter (Thermo Scientific; 0.2 µm) and diluted with 5 ml of saline.
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bioRxiv - Neuroscience 2024Quote: ... followed by blotting with filter paper and plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 4° C and 100% Krios G2 or G4 microscope (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Isolates were inoculated into 3 ml of filter-sterilized phytase-specific medium (PSM) (Hosseinkhani, Hosseinkhani, 2009) supplemented with 0.5 mM of Sodium phytate (Fisher Scientific). The cultures were then incubated for 48 hours at 30°C in a shaker incubator set at 80 rpm ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was filtered through a 0.45µm filter and incubated at 4°C for 16 hours with 2mL HisPur Ni-NTA resin (Thermo Fisher Scientific). Beads were pelleted for 5 min at 700 x g ...
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bioRxiv - Biophysics 2024Quote: ... The fatty acid:BSA mixture or a BSA vehicle control was then diluted in DMEM or IMDM containing 10% FBS to the desired concentration and filtered through a 0.2 µM PES filter (Fisher Scientific) before use ...
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bioRxiv - Immunology 2024Quote: ... Cell suspensions were passed through 40-μm filters and counted with a hemocytometer loaded into a Contess 3 (ThermoFisher Scientific) using Trypan Blue to exclude dead cells ...
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bioRxiv - Immunology 2024Quote: Bat cells were isolated from spleens by generating a single cell suspension using a 100-µm filter and red blood cells were removed using RBC lysis buffer (Invitrogen). Splenocytes were then cryopreserved in freezing medium (10% DMSO and 90% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... For the high-resolution AKIRIN2:IPO9 structure determination data collection was performed on a 300 kV Titan Krios G4 equipped with a cold field emission gun and a post-column Selectris energy filter (ThermoFisher) with a 10 eV slit width and a Falcon 4 or Falcon 4i direct electron detector (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... The dissociated cells were added at 200,000 cells/well to 6-well plates containing astrocyte-coated coverslips in filter-sterilized NBA plus [Neurobasal-A medium (Gibco) supplemented with 1X Glutamax (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... viral particles were concentrated using Amicon filters (EMD, UFC910024) and formulated in sterile PBS supplemented with 0.001% Pluronic F-68 (Gibco, Germany). Virus titer was 8.2×1012 genome copies/ml measured by determining the number of DNase I resistant genome copies using qPCR (StepOne ...
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bioRxiv - Neuroscience 2024Quote: ... and the cells were plated into T-75 flasks containing filter-sterilized astrocyte media [DMEM media supplemented with glutamine (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The viruses were concentrated using Amicon filters (EMD, UFC910024) and then suspended in sterile phosphate-buffered saline (PBS) supplemented with 0.001% Pluronic F-68 (Gibco, Germany). Using an AAV titration kit (TaKaRa/Clontech) ...
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bioRxiv - Neuroscience 2024Quote: ... binned by a factor of 2 and filtered using a non-local means filter in Amira version 2021.2 (ThermoFisher Scientific). Segmentation of the tomograms was carried out using the semi-automated convolutional neural network protocol of EMAN2 (Tang et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Colonies were scraped off from each plate and collected for purification with the PureLink HiPure Plasmid Filter Maxiprep Kit (Invitrogen). Finally ...
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bioRxiv - Microbiology 2024Quote: ... Samples were clarified by centrifugation and the supernatant was filtered through a 0.22 µm spin filter (Fisher Scientific, Catalog #07200389). Samples were mixed in a 1:1 ratio of solution containing isotopically labeled standards of known concentration.
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bioRxiv - Microbiology 2024Quote: ... POC and PONc filters were exposed to HCl fumes (4h) before analysis using a CHNS elemental Analyzer (Thermo Fisher Scientific) calibrated with acetanilide (23) ...
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bioRxiv - Biophysics 2024Quote: ... Tomograms were collected at a Titan Krios 300 keV TEM equipped with an BioContinuum energy filter and K3 camera at 64,000x using Tomo5 software (ThermoFisher Scientific). The tilt series were recorded from −60° to 60° with a 3° increment in a dose-symmetric bidirectional acquisition scheme ...
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bioRxiv - Systems Biology 2024Quote: Seawater samples were filtered with a 0.20 μm Nalgene Prefilter Plus GFP+ 0.2 μm CA filter (Thermo Scientific, Waltham, MA), preserved with trace metal grade HNO3 ...
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bioRxiv - Cancer Biology 2024Quote: ... the media underwent clarification through centrifugation at 2500 × g for 30 minutes (Sorvall ST4 Plus Centrifuge Series) and filtration using 0.22 µm syringe filters (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... a Selectris X energy filter and a Falcon 4i direct electron detector operated by the software EPU (Thermo Fisher Scientific). A total of 64,606 micrographs in five sub-datasets at 0° ...
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bioRxiv - Immunology 2020Quote: ... The absorbance was measured at 450 nm using a 620 nm reference filter in a microplate reader (Thermo Fisher Scientific, USA). The antibody titers were expressed as U/mL.
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bioRxiv - Immunology 2022Quote: Cell culture supernatants were filtered through a 0.22 μm filter and cytokine levels were analyzed on a Luminex™ MAGPIX™ (Thermofisher) as previously described (57,59) ...
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bioRxiv - Bioengineering 2022Quote: Conjugation of biotinylated oligo DNAs and antibodies was achieved through DBCO-azide click chemistry reaction.24 The antibodies were concentrated to 1 mg/mL first using 10K MWCO centrifuge filters (Amicon; Thermo fisher) and were purified by 7K MWCO Zeba spin desalt columns (Thermo fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions of the digested mid-brains were prepared by sequential disaggregation by pulling the homogenate through syringe needles with progressively smaller bores (18G and 20G needles) followed by passage through a 40 μm nylon filter (Fisher Scientific). After centrifugation at 650 xg for 11 min ...
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bioRxiv - Cell Biology 2021Quote: ... Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific) following addition of 4 mg/mL polybrene (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and 5 μL of resuspended cells were diluted in 1 mL PBS through a 35 μm filter (Corning Falcon tube, Thermo Fisher). FACS DIVA software was used to collect 100,000 events for each sample ...
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bioRxiv - Genomics 2021Quote: ... and strained through a 40-μm filter (pluriSelect) then stained for 5 min with Propidium Iodide (1 mg/mL, ThermoFisher Scientific) at room temperature to label dying cells.
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bioRxiv - Cell Biology 2020Quote: ... Antibodies formulated in PBS only were concentrated using 0.5 mL 50KDa Amicon Ultra Filters (EMDMillipore, UFC510096) to 2 mg/ml and reacted with maleimide-PEG2-succinimidyl ester crosslinkers (ThermoFisher 22102) for 2 hours at 4 °C ...
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bioRxiv - Physiology 2021Quote: ... The grids were blotted for 1-1.5 s with an ashless Whatman® Grade 540 filter paper in a Vitrobot Mark IV (ThermoFisher Scientific) and rapidly plunged into liquid ethane ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow cells were then passed through a 70-μm filter with 1% RPMI and red blood cells lysed with ACK lysis buffer (Life Technologies) and cell counts determined as above ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA from the overnight cultures was harvested via extraction with the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific), and insert sequence was confirmed by Sanger sequencing at the on-site DNA Sequencing Facility (Dept ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proboscis was inserted into a filter tip ART (Molecular BioProducts, USA) with 20 μL of fetal bovine serum (FBS; Glico by Life Technologies). Saliva was collected for 30 min and stocked in tubes containing 130 μL of cell culture medium (L-15 Medium ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were resuspended in 100-400 µl ST buffer without RNAse inhibitor and filtered with a 40 µm mesh filter attached to a FACS tube (Fisher Scientific). The nuclei concentration and dissociation quality was then determined in a 5 µl aliquot using Neubauer counting chambers (Bulldog Bio ...
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bioRxiv - Neuroscience 2020Quote: ... and insoluble material was removed by centrifugation and passage through a 0.22 μm Spin-X filter column (#8160, Thermo Fisher Scientific). Supernatants were incubated with Glutathione Sepharose (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Microbiology 2020Quote: ... The initial stock from BEI was passed through a 0.45 μM syringe filter and 5 μl of this filtered stock was inoculated onto ~80% confluent T175 flasks (Nunc, Roskilde, Denmark) of Vero-E6 cells to produce passage 1 of the virus ...
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bioRxiv - Neuroscience 2022Quote: ... cells grown at confluence on filters were incubated with 10 µg/mL Alexa Fluor™ 488 Ac-LDL (Thermo Fisher Scientific) in DMEM/F-12 supplemented with 1% BSA for 4h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Data were collected on a FEI Titan Krios microscope operating at 300 kV using a Falcon 4 direct electron detector with a Selectris imaging filter (10 eV slit width) (ThermoFisher Scientific) at the Astbury Biostructure Laboratory (Supplementary Table 1) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysate was filtered through a 0.45-μm syringe filter and loaded onto a gravity flow column containing Ni-NTA resin (Thermo Fisher Scientific) that was washed with 5-10 column volumes of wash buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: Ten milliliters of each water sample and Milli Q water (Blank) were filtered through a Nalgene™ 0.22 µm syringe filter (Thermo Scientific) to remove suspended particulates ...
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bioRxiv - Immunology 2021Quote: ... Lymph nodes and spleen were mechanically pushed through 70 μm nylon mesh cell filters followed by RBC lysis with ACK (ammonium-chloride-potassium) lysing buffer (ThermoFisher Scientific) solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual cells were characterized for forward and side scatter and DNA content was determined in 10,000 cells as measured by flow cytometry (FACS; excitation at 488 nm, emission measured using 600 nm bandpass filter) with an Attune cytometer (Thermo Fisher) or Novocyte cytometer (Agilent) ...
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bioRxiv - Physiology 2021Quote: ... Excess moisture was drained with filter paper and the imaging of the air-dried grids was done in a TEM Morgagni 268 (Thermo Fisher) operated at 100 kV ...
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bioRxiv - Biochemistry 2021Quote: ... 595 filter paper before being plunge frozen in liquid ethane using a Vitrobot Mark IV system (Thermo Fisher Scientific Inc., USA). Cryo-EM images of frozen-hydrated BsClpP particles were collected at Korea Basic Science Institute with a Titan Krios TEM system (Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2020Quote: ... samples were proteolysed by a modified filter aided sample preparation 57 and eluted peptides were analysed by LC-MSMS on a QExactive HF mass spectrometer (ThermoFisher Scientific) coupled directly to a UItimate 3000 RSLC nano-HPLC (Dionex) ...
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bioRxiv - Genomics 2022Quote: ... the CPG was filtered through a micro spin centrifuge filter with pore size of 0.2 μm and the resulting filtrate was evaporated to dryness on a SpeedVac (Thermo Fisher Scientific). The residue was dissolved in 0.75 mL of 3% acetonitrile/water mixture and filtered through a micro spin centrifuge filter ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were clarified by centrifugation at 15,000 rpm for 45 mins in a JA 25.50 rotor and the supernatant filtered using 1.1 µm syringe filters (Thermo Scientific #722-2000). Filtered supernatant was loaded onto a HisTrapTM HP column (Cytiva #17524802) ...
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bioRxiv - Cancer Biology 2022Quote: ... was prepared in DPBS (2 mg/ml) and filtered through a 0.22-micron Steritop filter (Millipore Fisher Scientific Catalog No-15770319). Gelatin was fluorescently labeled using Alexa Fluor 488 Protein labeling kit (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: ... and the supernatant was filtered through 0.22 µm filter and then passed through a CaptureSelect™ CH1-XL Affinity Matrix column (ThermoFisher Scientific). Recombinant Fab protein was eluted with 50 mM acetate buffer at pH 5.0 and concentrated using Ultra-15 Centrifugal Filters (Millipore) ...
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bioRxiv - Microbiology 2022Quote: Twenty mL of Allobaculum CM was concentrated to 0.5 mL using a 50 kDa MWCO Amicon Ultra filter (Thermo Fisher Scientific), reconstituted to 20 mL using PBS and reconcentrated once more to 0.5 mL ...