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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for Recombinant Human GPD1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and protein concentration for each sample was determined using a BCA assay (Pierce Microplate BCA Protein Assay Kit, ThermoFisher, Waltham, MA). Equal amounts of protein were prepared using 4x NuPage sample buffer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet samples were analyzed for protein expression using SDS-PAGE and Western blotting coupled with a prestained protein marker-180kDa (Thermo-Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS before lysing with 350 ul RLT for total protein quantification using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Final pellets were resuspended in HNE buffer and protein concentrations were measured using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to the manual provided by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the lysates was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher, Cat. no. 23225). Western Blots ...
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bioRxiv - Cell Biology 2022Quote: ... 2019).The quality of co-expressed proteins were evaluated through resolving the protein bands on SDS-PAGE gel (Bolt 4-12%, Invitrogen™) Following the completion of protein expression ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of the lysed sEV preparations were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA), following the manufacturer’s protocols (Leung et al. ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatants were subsequently clarified with 50 µL of magnetic beads coupled to protein G (Dynabeads Protein G-LS10003D, Thermo Scientific) at 4°C for 1 h with agitation ...
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bioRxiv - Microbiology 2023Quote: ... on ice for 30 min and the protein concentration was determined using the Pierce™ BCA protein assay kits (Thermo Fisher) to ensure equal loading ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was measured to be 0.1 mg/ml by the BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, MA). To make cryo-specimen of Fab fragment ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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bioRxiv - Molecular Biology 2024Quote: ... Efficacy of the process and final protein purity was assessed by SDS-PAGE using NuPAGE 4-12 % Bis-Tris Midi Protein Gels (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: Antibody-bound complexes were then captured by incubation with Protein A sepharose CL-4B beads (Amersham, 17-0963-03) for rabbit antibodies or Protein G sepharose 4B beads (Invitrogen, 101241) for mouse antibodies (both 20 μL per 1 mL lysate ...
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bioRxiv - Genomics 2024Quote: ... Protein quantification for each fraction was done using the manufacturer’s protocol for Pierce 660 nm protein assay reagent (Thermo Scientific, USA). Each Fraction was subjected to iCLIP protocol as described in Huppertz et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the sample was divided into equal amounts of protein (1.5 mg of protein/pull-down) and incubated with 80 μl of pierce streptavidin magnetic beads (Thermo Fisher Scientific), O/N ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration of each lysate was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Cell lysates (20 μg ...
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bioRxiv - Neuroscience 2024Quote: PVN tissue biopsies were collected from fresh-frozen brain tissue and protein extracted using Neuronal Protein Extraction Reagent (N-PERTM, Thermo Scientific) for determination of CRH protein levels by Western Blot ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein concentration in the supernatant was determined using the Micro BCA protein assay kit (Thermo Scientific, Waltham, MA; Cat.: 23235). In the same supernatant ...
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bioRxiv - Cancer Biology 2024Quote: ... 25-30 µg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed at 4°C with RIPA buffer and protein concentration was determined with the Micro kit BCA Protein Assay (Thermo Scientific, Courtaboeuf ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Immunology 2023Quote: ... Equivalent amounts of total protein lysate were subjected to electrophoresis on NuPAG 4–12% Bis-Tris Protein Gels (Thermo Fisher Scientific) and proteins were transferred to polyvinylidene difluoride membranes (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations in the supernatants were measured using the EZQ® Protein Quantitation Kit (Molecular Probes, Inc. Eugene, Oregon, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The molecular migration position of transferred proteins was compared with the PageRuler Prestain Protein Ladder 10– 180 kDa (Themo Fisher Scientific). Signal quantification was performed using Fiji ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Values were normalized for total protein content in each well using a Pierce BCA Protein Assay Kit (Thermo Fisher, cat# 23225) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The protein in the final sample was quantified using the Qubit Protein Assay Kit with the Qubit 2.0 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... The protein concentration of the supernatant was determined by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific). 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K ...
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bioRxiv - Microbiology 2022Quote: ... Samples were filtered through nitrocellulose membranes to remove the beads and protein was quantified using a Qubit 2.0 fluorometer and a Qubit™ protein assay kit (Life Technologies). 50 μg of protein sample was suspended in 50 μl of 8 M urea buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The molecular weight of protein bands was estimated by comparison with the BenchMark™ Pre-stained Protein Ladder (Thermo Fisher Scientific). The abundance of the 3xFLAG-ZF proteins were quantified from the band intensities also using Image Lab Software ...
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bioRxiv - Molecular Biology 2023Quote: ... protein in the supernatants was quantified using Pierce™ Bovine Serum Albumin (BCA) Protein Assay Kit (Thermo Fisher Scientific, Cat. 23225).
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bioRxiv - Physiology 2022Quote: ... Twenty μL of the crude extract was removed for protein quantification by a Bradford protein assay according to manufacturer’s instructions (Thermo Scientific Inc.). Next ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured by the bicinchoninic acid (BCA) assay using the Pierce BCA protein assay kit (ThermoFisher Scientific: 23225). Equal amounts of protein were loaded on 4 to 12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... and 150 µg of purified PPSA from SG and SEC fractions at 1 mg/mL total protein concentration (measured by a Pierce BCA protein assay, Thermo Scientific, according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies targeting proteins of interest or IgG isotype control (5ug) were incubated with 20ul of Dynabeads Protein G (Thermo Fisher Scientific) in buffer WB150 (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were measured using a Qubit 2.0 Fluorometer with a Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived NGN2 neurons were maintained in 6-well plates and were lysed on day 21-24 for membrane protein extraction using the Mem-PER Plus Membrane Protein Extraction kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The collected supernatant was used to measure protein concentration using the BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used to determine the protein concentration by BCA assay according to the manufacturer’s instructions (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Debris was removed by centrifugation (4°C, 30 min, 20’000 g) and total protein concentrations measured using the Bradford protein assay (Thermo Fisher Scientific). Typically ...
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bioRxiv - Microbiology 2023Quote: ... a volume equivalent to 250 µg protein (Quantification: 660 nm Protein Assay with ionic detergent compatibility reagent; Thermo Scientific, 22660 & 22663) was transferred to 15 mL centrifugation tubes and filled up with 1% SDS in 50 mM Na2HPO4 to a total volume of 920 µL ...
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bioRxiv - Neuroscience 2023Quote: ... Protein levels of the brain tissue were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: Cell Lysis Buffer (CST) was used to extract protein lysates and the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific) was used for fractionation according to manufacturers’ instructions ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... An aliquot (25□l) of this lysate was used for protein quantitation using Pierce Coomassie protein assay kit (cat#23200, ThermoFisher scientific). The remaining lysate was centrifuged at 5000xg at 4C to pellet debris ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was used for the enrichment of biotinylated proteins using100 μl Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher) overnight at 4 °C ...