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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for Mouse Anti Canine Distemper Virus Surface Envelope Antibody 8 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Biophysics 2022Quote: ... The surface was then incubated with a mixture of polyclonal rabbit anti-BSA IgG (Cat# A11133; Invitrogen, Waltham, MA) and monoclonal mouse anti-BSA IgG-1 (Cat# MA182941 ...
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bioRxiv - Microbiology 2019Quote: The PfCHC-2xFKBP-GFP soluble extract in buffer B was incubated with 4μl recombinant anti-GFP nanobodies covalently coupled to surface-activated Epoxy magnetic beads (Dynabeads M270 Epoxy, ThermoFisher) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Immunology 2024Quote: ... The following fluorochrome-labeled anti-human Abs were used for surface staining: PerCP/Cy5.5-CD19 (Invitrogen, #45-0198-42), APC-CD27 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... FcR receptor blocking antibodies were added for 15 min at 4°C and surface staining antibodies added together with live/dead stain (Fixable Live/Dead stains Thermo Fisher) and incubated for 20 mins at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... Samples were stained with surface antibody-cocktails for 20min at 4°C followed by fixable live/dead cell stain (Molecular Probes-Invitrogen). Cells were then fixed and permeabilized (eBioscience Transcription Factor Staining Buffer Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The surface concentration of the antibodies was quantified as previously described using a bicinchoninic acid assay (BCA) kit (Thermo Fisher 23227)36.
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bioRxiv - Immunology 2020Quote: ... Cells were then mixed with 40 μL of phenotype panel containing surface marker antibodies (Supplementary Table 3) and a dead cell marker (LIVE/DEAD Fixable Near-IR; Invitrogen L10119) (final dilution 1/1000) ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions were stained with HBSS containing extracellular surface antibodies and Live/Dead Fixable Blue Dead Cell Stain (Life Technologies). After extracellular staining for 25 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... Samples washed again with FACS buffer and then stained with labeled cell surface antibodies including CD11b-APCef780 clone M1/70 (Thermo Fisher Scientific Cat# 47-0112-80 ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700 ...
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bioRxiv - Immunology 2023Quote: ... in PBS for 10 min prior to addition of the relevant surface marker fluorochrome-conjugated antibodies in FACS buffer (PBS, 2% heat-inactivated FCS) supplemented with Super Bright staining buffer (Thermo Fisher). Following 30 min of incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression of surface molecules of T cells were determined by labeling T cells with fluorochrome conjugated monoclonal antibodies: CD4-AlexaFlour532 (Thermo Fisher), CD3-Pacific Blue ...
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bioRxiv - Synthetic Biology 2023Quote: ... Intracellular flow cytometry analysis was performed by first antibody staining cells for surface markers and live/dead status using LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen, L23105), followed by permeabilization using the Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated with the 1:1000 diluted secondary antibodies (goat anti-mouse AlexaFluor® 488-conjugated or AlexaFluor® 568-conjugated; Thermo Fisher Scientific, Waltham, MA, USA) for one hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher, diluted 1:5000 in TBS-T). After incubation with the secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with secondary anti-mouse AlexaFluor633-conjugated antibody (dilution 1:200 in incubation buffer; Molecular Probes, cat #: A-21052, Eugene, OR, USA) for 1 h in a darkened chamber at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies and the optimal dilutions and concentrations used are as follows: AF647-conjugated donkey anti-mouse IgG (Thermo Fisher, A-31571, 1:500, 4 µg/mL), AF647-conjugated donkey anti-rabbit IgG (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa 488 conjugated Claudin-5 (1:500; Invitrogen, 35-358-8), rabbit anti-CCL2 (1:500 ...
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bioRxiv - Immunology 2021Quote: 293T cells were transfected with full-length envelope expressing plasmid using lipofectamine2000 (ThermoFisher, catalog no 11668027) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... pSPAX2 packaging vector and pVSVG envelope vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) as described (73) ...
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bioRxiv - Cell Biology 2019Quote: ... and the lentiviral envelope vector VSVg were transfected into HEK 293T cells with TurboFect (Life Technologies). Transfected HEK 293T cells were incubated for two days before the virus-laden medium was transferred to HMECs or K562 cells ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Biophysics 2023Quote: ... and a BG505 envelope SOSIP were expressed in FreestyleTM 293-F cells (ThermoFisher Cat No. R79007). Before transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... ZO-1 (mouse 1:100, Invitrogen, 339100). Nuclei were counterstained with DAPI (1 µg/ml from a 1 mg/ml stock in DMSO ...
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bioRxiv - Microbiology 2022Quote: ... and Alexa-488 conjugated chicken anti-goat or goat anti-rabbit antibodies (1:1000 dilution) (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... secondary anti-rat-Alexa Fluor 594 and anti-rat-Alexa Fluor 647 antibodies (1:500, Life Technologies) were used to visualise the HA-tagged channels.
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bioRxiv - Molecular Biology 2023Quote: ... Anti-rabbit and anti-goat secondary antibodies conjugated with Alexa-Fluor 488 (Invitrogen, Cat# A11034, 1:2000) or Alexa-Fluor 555 dyes (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... This was followed by secondary antibody incubation (Donkey anti-Rabbit Secondary Antibody, Alexa Fluor 594, 1:1000, ThermoFisher) for one hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then stained with primary antibody anti-cTnT (Lab Vision; 1:200) and secondary antibody (Thermo Fisher; goat anti-mouse ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with primary antibody (1/400 diluted rabbit anti-BACH2 antibody (PA5-23642, ThermoFisher Scientific) and 1/5’000 diluted rabbit-anti-GAPDH antibody (ab9485 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies included AlexaFluor488-conjugated and AlexaFluor594-conjugated secondary antibodies (goat anti-rabbit IgG, 1:500; ThermoFisher Scientific). After staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The secondary antibodies used were Alexa fluor 488-conjugated goat anti-rabbit antibody (A11008; Invitrogen; dilution 1:1000) and Alexa fluor 594-conjugated goat anti-mouse antibody (A11005 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Antibody staining was performed following in situ hybridisation with guinea pig anti-insulin antibody (Invitrogen, diluted 1:1000) and rabbit anti-glucagon antibody (ZYMED ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with primary antibodies and subsequently with the corresponding biotinylated secondary antibodies (1:1000 Goat anti-rabbit, Invitrogen). Signal amplification was performed using the ABC complex (Vectashield) ...
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bioRxiv - Neuroscience 2022Quote: ... the secondary antibodies donkey anti-chicken Alexa 488-conjugated antibody (1:1000, cat #: A78948 ThermoFisher Scientific Invitrogen, USA), donkey anti-mouse Alexa 488-conjugated antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... antibodies were incubated at 4°C overnight followed by secondary antibodies anti-rabbit Alexa 488 (Invitrogen, 1:500), anti-mouse Alexa 594 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cell were then immunostained with anti-primary antibodies used at 1:500 and then AlexaFluor conjugated antibodies (Thermofisher) used at 1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... the secondary antibodies donkey anti-chicken Alexa 488-conjugated antibody (1:1000, cat #: A78948 ThermoFisher Scientific Invitrogen, USA), donkey anti-mouse Alexa 488-conjugated antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-SYCP3 antibody (1:1,000) (24) follower by applying the Alexa fluorescence-labeled secondary antibodies (Thermo Fisher). PNA-Cy5 (Vector laboratories ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with 1:750 secondary antibodies (Alexa Fluor 488-conjugated goat anti-rabbit antibody, A11008, Invitrogen) at 37 °C for 1.5 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) or Alexa Fluor 488 Goat Anti-rabbit IgG (H/L ...
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bioRxiv - Cancer Biology 2024Quote: ... The secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) and Alexa Fluor 488 Goat Anti-mouse IgG (H/L ...
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bioRxiv - Neuroscience 2024Quote: ... Appropriate secondary antibodies were used in antibody dilution solution (1:500, goat anti-guinea pig Alexa 594, Invitrogen A11076 ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Biochemistry 2024Quote: The cell surface expression of wild-type and mutant hGPR30-HA was analysed via cell surface protein isolation using a Cell Surface Protein Isolation Kit (#89881, Thermo Scientific™) followed by western blotting ...
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bioRxiv - Neuroscience 2023Quote: ... in blocking solution with the addition of the following secondary antibodies (dependent on primary antibody used, all secondary antibodies were used at 1:1000): donkey anti-goat A647 (Invitrogen A-21447), donkey anti-rabbit A488 (Invitrogen A-21206) ...