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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... supplemented with 9% foetal bovine serum (FBS) (10099, Gibco™ ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained using SYTO 9 (Thermo Fisher Scientific) and the fluorescent intensity was measured using Tecan microplate reader at 485 nm excitation and 530 nm emission wavelength ...
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bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
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bioRxiv - Genomics 2020Quote: ... and RNA integrity was estimated by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at −80⁰C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated for with varying concentrations of BCNU (0 to 2 mM) for an additional 48 h before incubation with Calcein AM dye (2 μM) (Invitrogen; Cat# C1430, Grand Island, NY) at 25°C for 30 m ...
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bioRxiv - Genomics 2021Quote: ... RNA Polymerase II 8WG16 (Covance #MM2-126R, 2 µg/ChIP) and Phospho-RNA pol II CTD (Ser5) (Thermo Fisher Scientific #MA1-46093, 2 µg/ChIP). ChIP assays for qPCR quantification were performed using primers shown in Table 2 ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed for 2 genes of interest and 2 internal controls (Suppl Figure S1) using Taqman® gene expression assays (Thermo Fisher, Foster City CA) and Taqman® Fast Universal Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Cells were starved in FCS-free DMEM for 2 hours prior to experiments and labeled during the last 20 minutes of starvation with 2 µM CellTracker™ Red CMTPX and 2 µg/ml Hoechst 33342 (both from Molecular Probes, Eugene, OR, USA). A gap was made in the cell monolayers of every well using an HTS Scratcher (Peira ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was determined by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at -80°C until further use ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were reduced with 2 µL of 200 mmol/L Tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Thermo Scientific; final concentration of 14.8 mmol/L) and incubated at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... were calculated using RQ-Min = 2-(ΔΔCt + T *SD(ΔCt)) and RQ-Max = 2-(ΔΔCt - T *SD(ΔCt)) from triplicate wells as suggested by Applied Biosystems (manufacturer of qPCR machines). Results shown are representative of multiple independent experiments with similar results.
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2024Quote: ... To precipitate the RNA, 250 μL of 2-propanol (AppliChem, Darmstadt, Germany) and 2 μL of GlycoBlue Coprecipitant (15 mg/mL) (Thermo Fisher Scientific, Waltham, MA, USA) were added to each sample ...
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bioRxiv - Microbiology 2024Quote: ... for 2 min before separation on an Easy-Spray™ C18 nano column (75 µm x 150 mm, 2 µm, Thermo Fisher Scientific, Waltham, MA) with a 107 min gradient at a flowrate of 0.3 µL/min ...
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bioRxiv - Cell Biology 2023Quote: A live/dead assay on the cell-laden GelMa constructs (n=6 per condition) was performed using a LIVE/DEADTM Cell Imaging kit (Invitrogen, Waltham, Massachusetts, United States) 144 h after Gel synthesis ...
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bioRxiv - Molecular Biology 2021Quote: ... N (Invitrogen #MA5-35943), Flag (Sigma # F3165) ...
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bioRxiv - Cell Biology 2024Quote: ... N-Hydroxysuccinimide (Invitrogen, A37573) was diluted in 0.2 M sodium bicarbonate to a final concentration of 50 µg/mL and applied to cells at RT for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Molecular Biology 2019Quote: Cells at 60% confluence were transfected with 40 nM of mirVana™ miRNA mimic of miR-9-5p (miR-9-5p) (4464066, Ambion, Carlsbad, CA) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: Global proteomics for all comparative method testing was carried out (n=3 per group) on an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled to an Eksigent nanoLC-2D system through a nanoelectrospray LC − MS interface ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... blood was collected postmortem via heart puncture on day two after infection (n = 3 mice/treatment) and kept at −20 °C in RNAlater (Thermo Fisher Scientific). RNA was extracted using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA depleted using the GLOBINclear Kit ...
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bioRxiv - Molecular Biology 2022Quote: Global proteomics for all comparative method testing was carried out (n=3 per group) on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Neuroscience 2024Quote: A total of 12 WT and pink1 KO mice (N = 3 per sex/genotype) at age of three months were anesthetized and intracardially perfused with cold PBS (Invitrogen, 14190-235). Brains were removed and immediately flash-frozen in liquid nitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 hMSCs from 4 donors chosen randomly (2 male; B, G, and 2 female; C, D) were thawed and cultured in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) supplemented with RoosterNourishTM-MSC expansion medium (RoosterBio ...
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bioRxiv - Developmental Biology 2022Quote: ... and inhibitors (50 µM 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA; Fisher Scientific), 8 µM EHop-016 (Selleck Chemicals) ...
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bioRxiv - Bioengineering 2022Quote: ... and 1.5 μl of TEMED (N,N,Ń,Ń-tetramethylethylenediamine; 15524-010, Invitrogen) were added to 500 μl of gel mixes ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... The RNA was treated with 2 U DNase I (Life Technologies) for 30 min at 37°C and cleaned using the RNeasy Plant Mini kit (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... after 2 days of 1000 ng/mL doxycycline induction (Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... after 2 days of 1000 ng/mL doxycycline induction (Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... 2 ml Trypsin-EDTA (0.25%; Gibco, Thermo Fisher Scientific, Massachusetts, USA) was added and the flask was incubated at 37°C for 2-3 minutes to detach the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... and then transfected using 2 µL of Lipofectamine 2000 (Life Technologies) and 1.6 µg of DNA per well with the GFP-LC3B expression plasmids with or without scrambled shRNA or a mixture of five FYCO1-targeting shRNAs (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... For Glucose uptake analysis 2-NBDG glucose-uptake marker (Thermofisher Scientific) was used ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher) was used for dual labeling ...
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bioRxiv - Cell Biology 2020Quote: ... After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope ...