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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated using a probe sonicator and protein concentration was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227). Samples were prepared in 1X Laemmli buffer (2% SDS ...
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2022Quote: ... The antibody-protein complexes were then captured by addition of Protein G magnetic Dynabeads (5 μL beads/μg antibody; Thermo Scientific, cat. no. 13424229) followed by further incubation for 1 h at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were subsequently re-solubilized in 40 uL of 5% SDS (v/v) and assayed for protein using the PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). 130-170ug of protein/sample (in a total volume of 33 uL) ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... The supernatant (islet sample) was collected and the total protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The total protein concentration was determined by BCA assay and 500 μg of total proteins was incubated for 1 hour (4°C, rotary agitation) with 25 μl of Pierce™ Protein A Agarose beads (ThermoFisher, cat. no. 20333), centrifuged at 2500 g for 3 minutes at 4°C and the supernatant recovered as pre-cleared lysate ...
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bioRxiv - Neuroscience 2023Quote: ... and ventral hippocampus were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein content was measured in each well at the end of the assay using the Qubit Protein Assay Kit (Molecular Probes, Eugene, OR, USA). Oxygen consumption rate (OCR ...
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bioRxiv - Biochemistry 2022Quote: ... were centrifuged at 16,000 g for 30 minutes at 4 °C and the supernatant was collected for protein estimation using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific; Cat# 23227). Equal amounts of liver protein (100 μg ...
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bioRxiv - Cell Biology 2022Quote: ... The intensity values were normalized by the total protein in each sample (determined by the Pierce™ BCA Protein assay kit, ThermoFisher Scientific, cat# 23225) before they were used for quantitative analysis.
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bioRxiv - Cancer Biology 2023Quote: ... for 2hours at 4°C and this was followed by precipitation of the protein-antibody complex with dynabeads protein G (Invitrogen, #300D, Carlsbad, CA, USA) for 2h in 4°C on rotator ...
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bioRxiv - Plant Biology 2023Quote: ... The nuclear and cytosolic fractions were boiled in 1X Laemmli buffer (Laemmli, 1970) for 10mins at 100°C and protein was quantified using BCA compatible protein kit (Thermo Fisher Scientific, Whatham, USA). Fifty micrograms of protein were loaded onto a 4-20% gradient SDS-PAGE denaturing protein gel as described above ...
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bioRxiv - Cell Biology 2023Quote: ... The differential level of nascent protein synthesis was quantified using Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen, Cat. # C10428) according to the manufacturer’s protocol on a BMG FLUOstar Omega Microplate Reader ...
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bioRxiv - Systems Biology 2024Quote: ... Protein-containing supernatant was transferred to a new tube and protein concentrations was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat #23225) following the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and kept on ice and protein concentration measured with a Qubit™ protein BR assay kit (Thermo Fisher Scientific, Waltham, MA) and samples stored at − 80°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected and protein concentration was determined using the Pierce™ BCA protein assay kit following manufacturer’s instructions (Thermo Fisher Scientific, Massachusetts, USA). Each sample was separated by non-reducing SDS-PAGE with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was transferred into a new tube and the total protein concentration was measured with the BCA protein assay (Thermo Fisher Scientific, Waltham, MA). A panel of 13 cytokines was quantified with a bead-based ELISA kit (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... Total IgG was purified from plasma samples of pre-immune and post-immunized rabbits via Protein A affinity chromatography (Pierce™ Protein A Agarose, Thermo Fisher Scientific, USA) as per manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: Isolation of sarcoplasmic and sarcolemmal protein were initially isolated from muscle tissue using a high-fidelity membrane protein extraction kit according to the manufacturer’s recommendations (Mem-PRE Plus Membrane Protein Extraction Kit; Thermo Fisher Scientific; Waltham, MA, USA). This process yielded solubilized sarcoplasmic and sarcolemmal protein isolates.
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bioRxiv - Neuroscience 2024Quote: ... A fraction of this neuronal mixture was taken to measure protein concentration through Pierce ™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, A53225) and the rest was used to isolate the biotinylated synaptic cleft proteins following the steps specified in Loh et al.24 In this sense ...
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bioRxiv - Microbiology 2020Quote: ... All antibodies were also incubated with Dynabeads Protein G (Invitrogen) that served as reference binding for all antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and Pierce Protein G magnetic beads (ThermoFisher, 88847, Lot: SI253639) were used for IP ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were treated with DNase I (Invitrogen, Cat # 18047019) at 40 U/ml for 1 hr at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... as indicated by a protein ladder (PageRuler Prestained, Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... 0.15% (v/v) Tween-20 and protein inhibitors (Thermo Fisher) were used to prepare cell lysates ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were quantified with a BCA Kit (Thermo Fisher) and normalized ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was quantified by BCA assay (Thermo Fisher; 23225). Lysates were diluted in 4X LDS sample buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies were coupled to Protein G Dynabeads (Thermo Fisher Scientific) following the Manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies were coupled to Dynabeads Protein G (Thermo Fisher Scientificc) following the Manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 2 hours incubation with Protein A dynabeads (Invitrogen). Immunoprecipitated material was sequentially washed with buffer containing low salt (150 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... and protein concentration measured using the BCA assay (Thermo Scientific). Samples were normalized ...
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bioRxiv - Cell Biology 2020Quote: ... PageRuler Plus Prestained Protein Ladder (#26619 ThermoFisher Scientific, Freemont, CA) was applied with the following molecular sizes ...
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bioRxiv - Cell Biology 2020Quote: ... quantified using the Pierce BCA Protein Assay kit (ThermoFisher Scientific). Data from 3 independent experiments were averaged and the luminescence expressed as a percentage of the control.
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bioRxiv - Cell Biology 2020Quote: ... Lysate concentration was measured using Qubit Protein Assay Kit (Invitrogen) and Qubit 4 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualized by ECL substrate (ThermoFisher Scientific) and exposed under ChemiDoc XRS System (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were denaturated in LDS loading buffer (Life Technologies) supplemented with 200mM DTT and boiled for 10 min at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by NuPAGE 4%–12%gradient gels (Invitrogen), transferred onto PVDF membranes (Immobilon®-P ...
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bioRxiv - Cell Biology 2020Quote: ... proteins in the lysate were digested by Proteinase K (Ambion), RNA was cleaned up using SPRI beads (GE ...
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bioRxiv - Immunology 2021Quote: ... SeeBlue™ Plus2 Pre-stained Protein Standard (Thermo Fisher Scientific) was used as a molecular weight marker ...
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bioRxiv - Immunology 2021Quote: ... This was followed by addition of Protein G Dynabeads (Invitrogen) and incubation for 4 hours at 4°C with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... with BSA protein standards and AccuSkan GO spectrophotometer (Fisher Scientific). Mixed with NuPAGE LDS Sample Buffer (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... Protein was transferred onto an iBlot nitrocellulose membrane (ThermoFisher, IB301002) using the iBlot system (ThermoFisher) ...
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bioRxiv - Physiology 2020Quote: ... The proteins were transferred onto a nitrocellulose membrane (Thermo Scientific). Following transfer ...
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bioRxiv - Physiology 2020Quote: ... protein concentration was determined using a BCA assay (Thermo Fisher). Samples were prepared in SDS Tris-Glycine Buffer (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μL of pre-washed protein G Dynabeads (Life Technologies) were added to the extract ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were then blotted onto PVDF membranes (Thermo Fisher Scientific) using vertical wet transfer at 100V for one hour at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using SuperSignal West Femto substrate (Thermo Fisher) and imaged using the FluorChem R system (Biotechne) ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μL of protein A/G Agarose (#20421, Thermo Scientific) were added to each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysates were loaded with LDS Sample Buffer (Invitrogen, NP0007) and Reducing Agent (Invitrogen ...