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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 2 mM L-glutamine (Gibco), and 1% penicillin/streptomycin (10,000 units/mL and 10,000 µg/mL respectively ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM L-glutamine (Invitrogen), non-essential aminoacids (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... 2 mM EDTA (Fisher Scientific), 1 mM DTT (American Bioanalytical) ...
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bioRxiv - Immunology 2024Quote: ... 55 μM 2-mercaptoethanol (Gibco), and 1% antibiotic-antimycotic solution (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM L-glutamine (Gibco) and utilized within 20 passages after thawing from lN2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2% B27 (#17504-044, Gibco), 200mM L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM L-glutamine (Gibco) and grown at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2024Quote: ... and 2% Sodium Pyruvate (Gibco) for viral packaging ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM L-glutamine (Gibco), 1 mM pyruvate ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM L-glutamine (ThermoFisher) and ROCK-inhibitor (Thiazovivin ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1 mM 2-mercaptoethanol (Gibco), 1% MEM non-essential amino acids (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B27 (Fisher Scientific, 17504044), 0.1% P/S ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... B27 (2%, Thermo Scientific, #12587010). Cells were dissociated to single cells by extensive pipetting or by using a 25 G needle ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... media was removed and cells were incubated with standard bath solution (SBS) containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; Thermo Fisher Scientific, Paisley, UK) and 0.01% pluronic acid for 1 h at 37 °C ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 500 mM KCl, 7.5% w/v RNAse free sucrose, 2 mM MgCl2, 75 mM NH4Cl, 2 mM puromycin [Fisher Scientific], and 2 mM DTT) by gentle pipetting ...
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bioRxiv - Immunology 2022Quote: ... and suspended in Fc Block for 5 min at RT prior to staining with a surface stain of following antibodies: CD3 (Invitrogen, 45-0031-82)/CD4 (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... and their derivatives were cultured in DMEM containing 1 g/l glucose supplemented with 10% heat-inactivated FCS (Fetal Calf Serum, Gibco, Cat#: 10270-106), 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Biochemistry 2020Quote: ... The concentration of glucose in filtrate was quantified using Glucose CII-Test Wako (FUJIFILM Wako Pure Chemical Corporation) by measuring the absorbance at 492 nm with a Thermo Scientific Multiscan® FC (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance at 340 nm was read every 12 s at 37°C using a Multiskan FC plate-reader (Thermo Scientific, Paisley, UK). Maximum absorbance ...
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bioRxiv - Microbiology 2020Quote: ... Then the resultant supernatants were transferred to a 96-well plate for the absorbance measurement at 492 nm with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA). RBCs treated with phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Planktonic cell growth was determined by quantifying optical densities at 595 nm using a microplate spectrophotometer (Thermo scientific multiscan FC: Cat no. 51119000). Plates were thoroughly washed three times with 200 µl of sterile PBS per well using a microplate washer (Micro wash 1100 from Global diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended at a concentration of 1 × 106 cells/mL in RPMI without FCS and labeled with CellTracker™ Blue CMAC dye (10 μM, Thermo Fisher, C2110) for 20 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: RPE-1 cells were maintained in DMEM media supplemented with FCS (10 % final concentration) and Penicillin Streptavidin-Glutamine (PSG; Gibco, 1 % final concentration). For starvation ...
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bioRxiv - Neuroscience 2021Quote: ... cells were collected from the flask, centrifuged (10 min, 150g, RT) and blocked for Fc receptors by using antibody CD16/32 (Invitrogen, 14-0161-82) (1:100) ...
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
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bioRxiv - Genomics 2024Quote: ... tissue-culture plates in DMEM (4.5 g/L glucose) with GlutaMAX-I supplemented with ES-tested 15% foetal calf serum (FCS, ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The absorbance reading was conducted at 595 nm using a microplate photometer (IVD model, MultiskanTM FC, Thermo Fisher Scientific Inc., MA, United States).
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bioRxiv - Systems Biology 2023Quote: Murine 3T3-L1 preadipocytes differentiated into mature adipocytes by first incubating confluent cells in induction-medium (DMEM GlutaMAX™, Gibco®, 10% FCS) containing 1.6μM insulin ...
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bioRxiv - Microbiology 2023Quote: ... Hungary) substrate and the optical density of the solution was measured at 450 nm using a Multiscan FC reader (Thermo Fisher Scientific Inc.). Blood samples were centrifuged after collection and the sera were kept at −70°C ...
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bioRxiv - Biophysics 2023Quote: ... setup alignment and quantifying confocal volume parameters were performed with fluorescence correlation spectroscopy (FCS) using ATTO425-COOH, ATTO488-COOH, Alexa Fluor 546 or ATTO655-COOH (ATTO-TEC GmbH, Siegen, Germany) (Alexa Fluor™, Thermofisher Scientific). Data was analyzed using PAM-FCSfit (Supplemental Figure S5A) ...
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bioRxiv - Biochemistry 2023Quote: ... Phage pools representing the libraries were cycled through rounds of binding selections with EPHA2-FN2-Fc fusion protein immobilised on Maxisorp Immuno plates (ThermoFisher, #12-565-135), as described previously [21] ...
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bioRxiv - Cell Biology 2023Quote: ... using either a WPA CO8000 Cell Density Meter (Biochrom, Cambridge, UK) or a Multiskan FC microplate reader with a 594 nm filter (Thermofisher, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). Proteins were then quantified with Image Studio Version 5.2.4 (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... The single cell suspension was incubated with 1:1000 Fc Block (AbLab) in 1X FACS Buffer and stained for surface markers before being fixed/permeabilized (Thermofisher 00-5523-00) for FoxP3 staining (invitrogen 11-5773-82 Table 2).
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bioRxiv - Cell Biology 2023Quote: ... plus 10 % FCS (PAA Laboratories; Pasching, Austria) and P/S (100 U/ml penicillin and 100 mg/ml streptomycin; Gibco, Thermo Fisher Scientific). Results with HeLa K are shown in Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... centrifuged for 5’ on 1.4 rpm and were incubated with Fc Blocker (1:100, in 1XPBS + 1%FBS, Thermo Fisher Scientific, Ghent, Belgium) for 30’ on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 50nM Aβ-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... After lysis of red blood cells with Gibco ACK lysing buffer and blocking of Fc receptors (Invitrogen CD16/CD32 clone 93, RRID AB_467133), cells were stained with live/dead aqua (Thermo ...
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bioRxiv - Microbiology 2024Quote: ... The isolated cells were resuspended in DMEM supplemented with 5% FCS and immediately plated on glass coverslips using Cytofunnels (Thermo Fisher Scientific, USA) and centrifugation at 1500g for 10 minutes with a Cytospin 4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... an internal solution containing 1 mM Fura-2 (K5-Fura-2, cell impermeable, Invitrogen F1200), 140 mM KCl ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Cells (70–80% confluent) were loaded with 2 μM Fura 2 AM (Molecular Probes, Oregon) in culture medium for 20 min at 37 °C in a humidified atmosphere of 95% O2 and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-Mercaptoethnol (2-ME; cat. num. 21985023; Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 0.1% L-ascorbic acid (ascorbate ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-Mercaptoethnol (2-ME; cat. num. 21985023; Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 0.1% L-ascorbic acid (ascorbate ...
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bioRxiv - Microbiology 2020Quote: ... Fluorescent stains (TOTO-1 iodide; 1 μM, Life Technologies, and ethidium homodimer-2 (EthHD-2); 1 μM ...