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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant baculovirus was generated in Sf9 cells using the Bac-to-Bac system according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Recombinant proteins were then expressed by infecting Hi5 cells for 96 h at 28°C ...
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bioRxiv - Cell Biology 2021Quote: ... or HIF1β were expressed in Sf9 cells that had been infected with recombinant baculovirus as described in the Bac-to-Bac® Baculovirus Expression System from Invitrogen.
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bioRxiv - Molecular Biology 2022Quote: ... intracellular ATP levels were determined by reaction of ATP with recombinant firefly luciferase and its substrate D-luciferin according to the manufacturer’s instructions (Thermo Scientific, A22066).
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bioRxiv - Physiology 2022Quote: ... Success of the transformation and choice of the recombinant plasmids was verified in LB agar cultures (Lennox L Agar, Thermo Fisher) containing Ampicillin (200 mg/mL ...
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bioRxiv - Immunology 2022Quote: The α−TD monoclonal antibody was produced in Sf9 insect cells by using a Bac-to-Bac recombinant baculovirus system (Invitrogen). The monoclonal antibody manufacturing process started with the revival ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids were utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen #10359016). Exponentially growing Spodoptera frugiperda 9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium #BELN12-730Q ...
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bioRxiv - Plant Biology 2020Quote: ... cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al. ...
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bioRxiv - Microbiology 2020Quote: All recombinant DNA manipulation was conducted according to standard procedures [18] and the recommendations of the enzyme manufacturer (Thermo Scientific, USA). Plasmid and chromosomal DNAs were isolated with Plasmid Miniprep (Evrogen ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using 50 ng of the template and the PCR mix contained 1U of recombinant Taq DNA Polymerase (Invitrogen™), 1x buffer ...
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bioRxiv - Microbiology 2020Quote: ... hACE2 receptor (hACE2 Recombinant Rabbit Monoclonal Antibody, 1:50, Thermo-Fisher, Cat#SN0754) and DAPI nuclear stain (1:20,000, Invitrogen, Carlsbad, CA). The primary antibodies were detected with goat anti-rabbit-AP developed with Permanent Red warp (mach3 Biocare Medical).
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bioRxiv - Biochemistry 2021Quote: The pFAST-bac1 vectors were used to produce recombinant baculovirus according to the protocol for the Bac-to-Bac system (Life Technologies), except using Insect GeneJuice (EMD Millipore ...
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bioRxiv - Immunology 2020Quote: ... CD8α+IEL were expanded in complete medium containing 50 ng/ml recombinant mouse IL-15/IL-15R complex (Thermo Fisher Scientific) on a tissue culture plate at the concentration of 5×105 cells/ml for 3 days ...
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bioRxiv - Immunology 2022Quote: ... Recombinant MARV-Angola GPdTM (IBT Bioservices) was tethered to Fluospheres NutrAvidin-Microspheres yellow-green or red (Thermo Fisher Scientific, Waltham, MA) using the EZ-link Micro Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Organoids were cultured in basement membrane extract (BME, Trevingen) and overlaid with growth medium consisting of murine recombinant epidermal growth factor (EGF 50 ng/ml, Life Technologies), murine recombinant Noggin (100 ng/ml ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... using conditions recommended by the supplier (1× PNK buffer without ATP, 2 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 2 h) ...
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bioRxiv - Molecular Biology 2022Quote: ... generally according to the suppliers’ recommendations (1× EPAP buffer, 1 mM ATP, 1 mM DTT, and 0.8 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 700µl FACS buffer with 3.5ul recombinant RNase inhibitor and Hoechst dye (Thermo Fisher, H3570, 1:2000) and incubated 4min on ice covered from light ...
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bioRxiv - Genomics 2022Quote: ... 2.5uL of the Slic Reaction is used for subcloning to generate a recombinant donor pFast vector with the Bac-to-Bac™ Baculovirus Expression System (Gibco) as per manufacturer instructions using TOP10 E ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg HexaPro) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Biochemistry 2023Quote: ... followed by staining with secondary anti-Rabbit antibodies (1:500) conjugated to Alexa Fluor 555 (Goat anti-Rabbit IgG, Superclonal Recombinant Secondary Antibody, Alexa Fluor 555, Thermofisher, # A27039) for 30 minutes on a shaker at Room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: 20µg of rhEPO and reference product Eprex® were treated with 20 units of recombinant PNGase F (Thermo Fisher Scientific, USA) at 37 ºC for hours ...
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bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
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bioRxiv - Cancer Biology 2023Quote: ... LN single cell suspension were stimulated for 5h at 37°C with 5ug/ml of LCMV-GP61-80 in the presence of 50 U/ml recombinant murine IL2 (Thermofisher P2747) and 1mg/ml Brefeldin A (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of cytokines in the sample was determined by a standard curve generated with recombinant IP-10 (Thermo Fisher Scientific) or rekombinant IFN-α (IFN-a2a ...
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bioRxiv - Genomics 2023Quote: ... HeLa cells were electroporated with the IDT Alt-R CRISPR-Cas9 system (recombinant Cas9, tracrRNA, crRNA) and paired gRNAs using the Neon system (ThermoFisher Scientific). Clonal lines were isolated using the limited dilution method in a 96-well plate format ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
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bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein concentrations of cell lysate supernatants were assayed using a BCA protein assay kit (Thermo Scientific). Protein samples were resolved on 8–12% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Waltham, USA). For long-term storage ...
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bioRxiv - Cell Biology 2019Quote: ... using 3–5 µg of primary antibody against each protein and protein-G magnetic Dynabeads (10003D, Invitrogen) and incubated overnight at 4°C on rotation ...
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bioRxiv - Genetics 2021Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris Protein gels (Thermo Fisher Scientific) and electrotransferred to 0.2 μm pore size nitrocellulose membranes (Amersham Protran Supported ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein concentration of Jz lysates was determined using the Bicinchoninic Acid protein assay (Thermo Scientific, US). Lysates were diluted to 2.5μg/μl in lysis buffer and 1xSDS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The supernatant was kept and the protein concentration quantified using the Qubit™ Protein Assay (Invitrogen, Q33212). 30 μg of protein was used per lane ...
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bioRxiv - Developmental Biology 2021Quote: ... and then bound to 50 μl of Protein A magnetic beads (Dynabeads Protein A, Thermo Fisher Scientific). The purified chromatin was then washed ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were collected and protein concentration was assessed with the Pierce BCA protein assay kit (ThermoFisher Scientific). 10 µg of protein from each sample was denaturated in Laemmli buffer (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: Cytoplasmic and nuclear protein extraction was performed using a commercial subcellular protein fractionation kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration of the supernatants was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). 20 μl TUBEs beads/IP were washed twice with TBST (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Protein lysates were quantified using the micro-BCA protein assay kit (23225, ThermoFisher Scientific, New York, NY) using BSA as standard ...
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bioRxiv - Immunology 2022Quote: ... Cellular debris were removed and supernatant protein concentration was determined using a BCA protein assay (Thermo Scientific). Disulfide bridges were reduced with 5 mM tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Immunology 2021Quote: ... Total protein concentration of BALF was measured using a Pierce™ BCA Protein Assay Kit (Thermo Fisher). BALF volume equivalent to 25 μg of protein was added to each well to normalise across groups accounting for any effects of imbalances in protein concentration due to treatments ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher Scientific, NP0336), and transferred to 0.45μm nitrocellulose membranes (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The protein content in the samples was estimated using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 µg of sonicated chromatin (protein content measured using BCA Protein Assay kit, ref# 23225, Thermo Scientific) was immunoprecipitated for 4h at 21°C on Pan mouse Dynabeads (Invitrogen ...