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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD proteins were either precomplexed with StrepMAB-Classic HRP (IBA) and secondary goat anti-mouse IgG Alexa Fluor 555 (Invitrogen) in a 4:2:1 molar ratio or conjugated with pA-LS NP in a 1:1 molar ratio ...
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD trimers or Fc chimeras were precomplexed with primary StrepMAB-Classic HRP (IBA) and secondary goat anti-mouse IgG Alexa Fluor 555 antibody (Invitrogen) applied at a 4:2:1 molar ratio ...
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bioRxiv - Neuroscience 2024Quote: ... slices were rinsed with PBS and incubated with the secondary antibody (goat-anti-mouse-Alexa Fluor 568 1:500, Invitrogen A-11004 ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen/ThermoFisher), and goat anti-rabbit IgG (H + L ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and incubated with secondary antibodies (Goat anti-mouse 555, Goat anti-rabbit 647 Invitrogen) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... or 647-conjugated donkey anti-mouse or anti-rabbit IgG (1:250; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 1:1000 anti-Sox2 polyclonal antibody overnight followed by 1:500 Alexa Fluor 488 and 1:500 Alexa Fluor 694 goat anti-rabbit IgG (Invitrogen, A-11034).
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 donkey anti-goat (A-11055) and Alexa Fluor 488 donkey antimouse (A-21202) were purchased from Molecular Probes (USA). Pioglitazone hydrochloride (E6910) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with donkey Alexa Fluor 555-conjugated antigoat and donkey Alexa Fluor 488-conjugated anti-rabbit antibodies (Thermo Fisher Scientific). For double infection ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue was incubated at 4° overnight with goat anti-rabbit Alexa Fluor 555 (ThermoFisher #A32732, RRID AB_2633281, 1:500; Alexa Fluor 488-conjugated phalloidin (ThermoFisher #12379, 1:100), and DAPI (ThermoFisher #D1306 ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were goat anti–rabbit or anti-chicken IgG conjugated with Alexa Fluor 546 for red color or Alexa Fluor 488 for green color (Thermo Fisher Scientific). Images were collected with an inverted microscope (Nikon Eclipse TE2000-U ...
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bioRxiv - Microbiology 2024Quote: ... anti-GFP rabbit polyclonal antibody conjugated to Alexa Fluor 488 and donkey anti-goat Alexa Fluor 568 (all 1:1000 from Life Technologies, Invitrogen) were incubated for 1 hour at RT in 3% BSA ...
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bioRxiv - Biophysics 2021Quote: ... Small-molecule stains such as Hoechst 33342 (1 µg/mL) and Alexa Fluor 647 Phalloidin (Life Technologies, 0.5 μM) were applied immediately prior to imaging for 10 min in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Plasma membrane and Golgi apparatus of transfected cells were stained with 0.5μg/ml lectin-HPA conjugated to Alexa Fluor 647 in PBS supplemented with 1% BSA (Life Technologies) and washed twice with PBS prior imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU staining was performed using a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were incubated with peanut lectin (PNA) conjugated to Alexa Fluor 647 (L32460, Thermo Fisher Scientific, 1:2,000) and DAPI (340-07971 ...
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bioRxiv - Cell Biology 2019Quote: ... permeabilized with 0.1% Triton X-100 and stained with Alexa Fluor 647-conjugated phalloidin (1:500) and Hoechst 33258 dye (1:10,000) (Invitrogen) to visualize F-actin and nuclei ...
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bioRxiv - Cancer Biology 2020Quote: ... EVs were stained with Alexa Fluor 647 Far red secondary antibody (1 μl of 1:20 antibody solution; Invitrogen) for 20 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were washed three times in 0.1% BSA in PBS and incubated with the corresponding secondary antibodies at 1:500 dilution for 1.5 hrs at RT (Alexa Fluor 488 and 647 nm; Invitrogen). Cells on glass coverslips were mounted with mounting media (Invitrogen ProLong Gold Antifade Reagent) ...
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bioRxiv - Bioengineering 2020Quote: ... and stained for 20 minutes on ice with 1 µg/ml Alexa Fluor 647-conjugated streptavidin (Thermofisher Scientific S21374). After two more washes with HyClone PBS (without BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Sharpened tungsten needles were used to lesion the lateral line nerve at the level of neuromast L1 and to deposit labeled dextran crystals (Texas Red, Alexa Fluor 647, or Cascade Blue, 3,000 MW, Invitrogen). Fish were unembedded and allowed to recover for at least 24hs before proceeding with further experimentation.
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bioRxiv - Neuroscience 2021Quote: ... we injected 40nl of cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (0.5%) or 647 (0.25%; Life Technologies) into the pulvinar ...
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bioRxiv - Neuroscience 2020Quote: ... A third monkey was injected with a cholera toxin subunit B-Alexa Fluor 647 conjugate (CTB-AF647; C34778, Invitrogen) at the quadrigeminal cistern ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU detection was performed by using Click-iT™ EdU Alexa Fluor™ 647 Imaging Kit (Thermo scientific #C10340) following manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were then dialyzed overnight in a Slide-A-Lyzer dialysis cassette (MWCO 10,000) before Alexa Fluor 647 maleimide labelling following the protocol described by the manufacturer (Invitrogen). Protein concentrations were measured using a Bradford assay (Biorad).
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS with 0.1% Triton X100 and incubated with the appropriate fluorescent secondary antibodies (1:1,000 Alexa-fluor 405, 488, 555, 647, Invitrogen) for 1 hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then rinsed for 5 min at RT (3X) with PBS and incubated with secondary antibody (Alexa Fluor 647 diluted 1:2,000 in blocking buffer; Invitrogen) for 45 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Neuroscience 2020Quote: ... they were washed 3 times in PBS and then incubated with the specific secondary antibodies (Alexa fluor 488, 594 and 647- 1:1,000; Invitrogen) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2020Quote: Recombinant GST-tagged PH-domain of PLCd detecting the membrane lipid PI(4,5)P2 was produced and conjugated to of amine-reactive Alexa Fluor 647 carboxylic acid succinimidylester (Invitrogen) as previously described49 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells labelled with EdU were visualised using a Click-iT EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... We stained for EdU with the Click- it EdU cell proliferation kit for imaging (Alexa Fluor 647 dye; Invitrogen) as described in the kit protocol ...
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bioRxiv - Cell Biology 2022Quote: ... EdU pulse was done by incubating the cells with EdU (1:1000) (2.52 mg/ml, Click-iT Plus EdU Alexa Fluor 647 Imaging Kit, # 15224959, Invitrogen) for 1 h at 37 °C before fixation with 4 % PFA.
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bioRxiv - Immunology 2021Quote: ... all PE labelled and prefusion SARS-CoV-2 S-Avi tag conjugated with streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2-specific IgG+ memory B cells were sorted and seeded on MSC (mesenchymal stromal cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Detection of EdU was performed using the Click-iT EdU Alexa Fluor 647 imaging kit (Thermo Fisher Scientific, C10340) in accordance with the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... EdU was labelled at this point using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific). Cells were then incubated with primary antibodies diluted in blocking solution overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Floating and attached cells were collected and incubated with Alexa Fluor 647 annexin V Ready Flow Conjugate (Thermo Scientific) for 15 minutes and 10 000 cells were analysed using a BD Accuri C6 flow cytometer.
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bioRxiv - Cancer Biology 2019Quote: ... EdU was fluorescently labelled with 0.5 μM Click-iT™ EdU Alexa Fluor™ 647 Azide (Invitrogen™ #A10277) in labelling buffer (100 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 5 min and labelled with Alexa Fluor 647-conjugated phalloidin (Invitrogen), during 45 min on the dark ...
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bioRxiv - Neuroscience 2019Quote: ... A second retrograde tracer (Cholera toxin subunit B, Alexa Fluor 647 Conjugate, Invitrogen, 50–60 nl, 1.0 mg/mL) was injected elsewhere to label cells projecting to a different cortical or subcortical area ...
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bioRxiv - Immunology 2020Quote: ... Neutrophil membranes were stained for 15 minutes with 2 µg/ml Alexa Fluor 647-conjugated Wheat Germ Agglutinin (ThermoFisher). Samples were concentrated and dried onto 1% agar pads ...
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bioRxiv - Developmental Biology 2020Quote: ... Fluorescent detection of incorporated EdU was performed following the manufacturer’s instructions using a Click-it EdU Alexa Fluor 647 Imaging Kit (Invitrogen) prior to primary antibody incubation.
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bioRxiv - Microbiology 2021Quote: ... and prefusion SARS-CoV-2 S with a biotinylated Avi-tag conjugated to Streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2 S-specific IgG+ memory B cells were sorted by flow cytometry via gating for PE negative and Alexa-Fluor 647 positive cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Corresponding Alexa-Fluor (−488, −568 and −647) secondary antibodies were used to reveal primary antibody binding (Thermo Fisher Scientific). For generating the plots shown in Figure 1d ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU labeling was carried out using the Click-iT Edu Alexa Fluor 647 (AF647) imaging kit (Invitrogen, Cat#C10340) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... followed by either EdU staining using the Click-iT EdU detection kit conjugated with Alexa Fluor 647 (ThermoFisher #C10340) or BrdU antibody staining ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU detection was then performed using Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher, C10424) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and prefusion SARS-CoV-2 S with a biotinylated avi tag conjugated to Streptavidin Alexa-Fluor 647 (Life Technologies). SARSCoV-2 S-specific IgG+ memory B cells were sorted by flow cytometry via gating for PE negative and Alexa-Fluor 647 positive cells ...
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bioRxiv - Immunology 2020Quote: ... The EdU+ cells were then stained according to the manufacturer’s instructions (Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit, Invitrogen). Using a CytoFLEX flow cytometer (Beckman coulter ...
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bioRxiv - Immunology 2020Quote: ... EdU staining was performed with a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies) per the manufacturer’s protocol ...