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8001 - 8050 of 10000+ citations for Anti NKCC1 Antibody since 2019

• ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

  Demis Menolfi, et al., bioRxiv - Genetics 2023

  Quote: ... cells were incubated with secondary antibody (goat anti-rabbit Alexa488, Thermo Fisher Scientific, A-11008, 1:300) for 1 h at room temperature in the dark ...
• Stability of dentate gyrus granule cell mossy fiber BDNF protein expression with age and resistance of granule cells to Alzheimer’s disease neuropathology in a mouse model

  Chiara Criscuolo, et al., bioRxiv - Neuroscience 2023

  Quote: ... donkey anti–mouse IgG-488 Alexa Fluor– conjugated secondary antibody was used (1:500; Cat#A21202, Invitrogen) in a solution of 2% DS and 0.3% Triton X-100 in TBS ...
• KLIPP - a precision CRISPR approach to target structural variant junctions in cancer

  Huibin Yang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the samples were incubated in secondary antibody (goat anti-rabbit IgG with Alexa Fluor 647, A21244, Invitrogen) for 1 h at 37°C ...
• Phosphate starvation decouples cell differentiation from DNA replication control in the dimorphic bacterium Caulobacter crescentus

  Joel Hallgren, et al., bioRxiv - Microbiology 2023

  Quote: ... followed by 1:5,000 of goat anti-rabbit secondary horseradish peroxidase-conjugated antibody (Thermo Fisher Scientific #31460) in TBST 0.3% skim milk powder ...
• FUS regulates RAN translation through modulating the G-quadruplex structure of GGGGCC repeat RNA in C9orf72-linked ALS/FTD

  Yuzo Fujino, et al., bioRxiv - Neuroscience 2023

  Quote: ... the samples were incubated with an Alexa 633-conjugated anti-rat antibody (A-21094, Thermo Fisher Scientific), or an Alexa 488-conjugated or Alexa 555-conjugated anti-rabbit antibody (A-11008 or A-21428 ...
• An integrated mechanistic and data-driven computational model predicts cell responses to high- and low-affinity EGFR ligands

  Paul J. Myers, Sung Hyun Lee, Matthew J. Lazzara, bioRxiv - Systems Biology 2023

  Quote: ... cells were incubated in-well with Alexa Fluor Plus 488 anti-rabbit secondary antibody (Thermo Fisher, A32790) and Hoechst nuclear stain (1:2,000 ...
• Hippocampal protein aggregation signatures fully distinguish pathogenic and wildtype UBQLN2 in amyotrophic lateral sclerosis

  Kyrah M. Thumbadoo, et al., bioRxiv - Neuroscience 2023

  Quote: ... but with primary antibody targeting polyGA detected by goat anti-mouse IgG1 Biotin (A10519, Thermo Fisher Scientific), followed by 3x 5-min PBS washes ...
• A Spatiotemporal Notch Interaction Map from Membrane to Nucleus

  Alexandre P. Martin, et al., bioRxiv - Molecular Biology 2023

  Quote: ... the cells were incubated with secondary antibody (Alexa Fluor Plus 647-conjugated anti-rabbit, ThermoFisher Scientific A32795) diluted in blocking buffer for 45 min at room temperature followed by three washes in DPBS ...
• The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in atrophic AMD

  Sayan Ghosh, et al., bioRxiv - Cell Biology 2023

  Quote: ... The flatmounts were then incubated with Anti-Rabbit Alexa fluor 555 secondary antibody (1:500) (A31572, Invitrogen) prepared in secondary antibody buffer (0.1% Tween 20 + 0.5% BSA in PBS) ...
• Coordination of Pickpocket ion channel delivery and dendrite growth in Drosophila sensory neurons

  Josephine W. Mitchell, et al., bioRxiv - Neuroscience 2023

  Quote: ... A fluorescently conjugated secondary antibody was used: goat anti-rabbit-Dylight 633 (1:500; catalog #35563, Invitrogen).
• Phenotypic Switching of Vascular Smooth Muscle Cells in Duchenne Muscular Dystrophy

  Wanling Xuan, et al., bioRxiv - Cell Biology 2023

  Quote: ... at 4 °C overnight respectively and anti- mouse secondary antibody conjugated to Alexa Fluor 488 (Life Technologies) at room temperature for 1 h ...
• TECPR1 provides E3-ligase like activity to the ATG5-ATG12 complex to conjugate LC3/ATG8 to damaged lysosomes

  Yingxue Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... Washed cells were incubated with secondary antibody anti-Rabbit IgG Alexa Fluor® 488 (Life Technologies, A11008), anti-Rat IgG Alexa Fluor®594 ...
• Nuclear MTHFD2 secures mitosis progression by preserving centromere integrity

  Natalia Pardo-Lorente, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following secondary antibodies were used: goat-anti rabbit 488 (Thermo Fisher Scientific; #A-11008; 1:1000) and goat-anti mouse 555 (Thermo Fisher Scientific ...
• Nuclear MTHFD2 secures mitosis progression by preserving centromere integrity

  Natalia Pardo-Lorente, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were used: Alexa Fluor 488 donkey anti-rabbit (Thermo Fisher Scientific; #A21206; 1:1000), Alexa Fluor 647 goat anti-rabbit (Thermo Fisher Scientific ...
• Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic identity and translation capacity

  Nicholas Raun, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Unc84::GFP labelled nuclei were then immunoprecipitated from homogenate with 1:200 anti-GFP antibody (Invitrogen: G10362) bound to magnetic beads (Invitrogen ...
• Structural alterations in the amygdala and impaired social incentive learning in a mouse model of a genetic variant associated with neurodevelopmental disorders

  Takeshi Hiramoto, et al., bioRxiv - Neuroscience 2023

  Quote: ... Free-floating sections were stained with a rabbit monoclonal anti-calretinin antibody (1:100, MA5-14540, Invitrogen/Thermo Fisher Scientific ...
• Human Immunodeficiency Virus 1 Capsid Uncoating in the Nucleus Progresses Through Defect Formation in the Capsid Lattice

  Levi B. Gifford, Gregory B. Melikyan, bioRxiv - Microbiology 2023

  Quote: ... The Alexa Fluor 568 goat anti-mouse IgG H+L antibody was purchased from ThermoFisher (Cat# A11004). The capsid binding inhibitor PF74 was purchased from Sigma-Aldrich (CAT# SML0835) ...
• Microglia at Sites of Atrophy Restrict the Progression of Retinal Degeneration via Galectin-3 and Trem2 Interactions

  Chen Yu, et al., bioRxiv - Immunology 2023

  Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
• The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

  Mizuki Morita, et al., bioRxiv - Developmental Biology 2023

  Quote: ... the samples were treated with 1:500 anti-rabbit IgG-Alexa 555-conjugated donkey antibody (Invitrogen A31572) and 1:500 anti-goat IgG-Alexa 488-conjugated donkey antibody (Invitrogen A11055 ...
• Rescue of secretion of a rare-disease associated mis-folded mutant glycoprotein in UGGT1 knock-out mammalian cells

  Gábor Tax, et al., bioRxiv - Cell Biology 2023

  Quote: ... Goat anti-Mouse IgG (H+L) Secondary Antibody DyLight 800 4x PEG (Thermo Fisher Scientific, MA, USA) was applied with 1:10,000 dilution factor in 1x TBST + 5% skimmed milk powder for 1 hr at room temperature ...
• Principles of assembly and regulation of condensates of Polycomb repressive complex 1 through phase separation

  Kyle Brown, et al., bioRxiv - Biochemistry 2022

  Quote: ... an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L) secondary antibody (1:1000; Life Technologies; A11011). After washing ...
• Transient loss of Polycomb components induces an epigenetic cancer fate

  V. Parreno, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The following secondary antibodies were used: donkey anti-goat Alexa Fluor 555 (1:1000, Invitrogen, A-21432), donkey anti-mouse Alexa Fluor 647 (1:1000 ...
• Surface tension drives neuronal sorting in magnetically engineered brain-like tissue

  Jose E. Perez, et al., bioRxiv - Bioengineering 2022

  Quote: ... spheroids were incubated in Alexa Fluor 488®-conjugated goat anti-mouse IgG secondary antibody (A11029, Thermofisher) at a 1:400 dilution in blocking solution for 2 hours at room temperature ...
• A paradox promoted by microglia cannibalism shortens the lifespan of developmental microglia

  Hannah Gordon, Zachary T. Schafer, Cody J. Smith, bioRxiv - Developmental Biology 2023

  Quote: ... The secondary antibody used was Alexa Fluor 647 goat anti-mouse (1:600, Thermo Fisher, A-21235). Staining was performed using the protocol by Nichols and Smith42 ...
• Structure and function of Plasmodium actin II in the parasite mosquito stages

  Andrea J. Lopez, et al., bioRxiv - Biochemistry 2022

  Quote: The secondary antibody used in the immunofluorescence experiments was Alexa-647 anti-rabbit obtained from Thermo Fisher Invitrogen ...
• Chemical factors induce aggregative multicellularity in a close unicellular relative of animals

  Núria Ros-Rocher, et al., bioRxiv - Biochemistry 2022

  Quote: ... aggregates were incubated with 1:2000 of goat anti-chicken Alexa Fluor 488 secondary antibody (#H11039, Invitrogen) for 30 min at 50 rpm orbital agitation at room temperature ...
• Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

  Paul Batty, et al., bioRxiv - Cell Biology 2023

  Quote: ... diluted as for the primary antibody (goat anti-mouse Alexa Fluor 488, Molecular Probes, A11001, 1:300) for 30 min at room temperature ...
• Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

  Paul Batty, et al., bioRxiv - Cell Biology 2023

  Quote: ... and visualised with a goat anti-rabbit Alexa Fluor 568 secondary antibody (Molecular Probes, A11011, 1:1000). HA-WAPL was detected using a monoclonal rabbit antibody against the HA-tag (Cell Signalling Technology ...
• Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

  Paul Batty, et al., bioRxiv - Cell Biology 2023

  Quote: ... and visualised with a goat anti-mouse Alexa Fluor 568 secondary antibody (Molecular Probes, A11004, 1:1000) or a goat anti-mouse Alexa Fluor 488 secondary antibody (Molecular Probes ...
• Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

  Paul Batty, et al., bioRxiv - Cell Biology 2023

  Quote: ... and visualised using a goat anti-rabbit Alexa Fluor 647 secondary antibody (Molecular Probes, A21244, 1:1000) or detected with a monoclonal mouse antibody (SantaCruz ...
• Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

  Paul Batty, et al., bioRxiv - Cell Biology 2023

  Quote: ... and visualised using a goat anti-mouse Alexa Fluor 568 secondary antibody (Molecular Probes, A11004, 1:1000). mEGFP-mAID-NIPBL and SCC1-mEGFP were detected and visualised with an anti-GFP nanobody booster (Chromotek ...
• Cooperative super-enhancer inactivation caused by heterozygous loss of CREBBP and KMT2D skews B cell fate decisions and yields T cell-depleted lymphomas

  Jie Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... followed by incubation with secondary antibodies Donkey anti-Rat IgG-AF488 (Invitrogen A21208, RRID:AB_141709, 1:500 dilution) and Donkey anti-Rabbit IgG-AF594 (Invitrogen A21207 ...
• Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination

  Yi-Chang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... sections were incubated with a goat anti-rabbit 488 secondary antibody (1:500; Cat# A-11034, ThermoFisher). Samples were imaged by a fluorescence microscope (Observer II ...
• Targeting the cell and non-cell autonomous regulation of 47S synthesis by GCN2 in colon cancer

  Marie Piecyk, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Secondary Antibody Alexa Fluor 488 Goat anti-mouse IgG (ref A-11029, Life Technologies, Carlsbad, California, USA) was used at 1:800 ...
• The plant nuclear lamina disassembles to regulate genome folding in stress conditions

  Nan Wang, et al., bioRxiv - Genomics 2022

  Quote: ... and 1:150 diluted Anti-rabbit Alexa Fluor 546-conjugated goat antibody (ThermoFisher, Catalog no. A-11035). For immunohistostaining ...
• Regulation of Nuclear Transcription by Mitochondrial RNA

  Kiran Sriram, et al., bioRxiv - Genomics 2023

  Quote: ... the cells were incubated with secondary antibody anti-mouse Alexa 488 (Thermo Fisher Scientific, Cat # A-11008) at 1:200 dilution for 30 min at room temperature ...
• A cell-type-specific multi-protein complex regulates expression of Cyclin B protein in Drosophila male meiotic prophase

  Catherine C. Baker, et al., bioRxiv - Developmental Biology 2023

  Quote: ... testes were incubated in secondary antibody (Alexa Fluor-conjugated donkey anti-mouse 488, 1:200, Molecular Probes) for 2 hours in the dark at room temperature ...
• IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion

  Steffen Klein, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cells were incubated with the secondary antibody ‘goat anti-Rabbit Alexa Fluor™ 633’ (ThermoFisher Scientific, Invitrogen) in a dilution of 1:2,000 in dilution buffer for 1 h at RT on a rocker ...
• IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion

  Steffen Klein, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cells were incubated with the secondary antibody ‘goat anti-Rabbit Alexa Fluor™ 633’ (ThermoFisher Scientific, Invitrogen) in a dilution of 1:2,000 in dilution buffer for 1 h at RT on a rocker ...
• IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion

  Steffen Klein, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cells were incubated with the secondary antibody ‘goat anti-Rabbit Alexa Fluor™ 633’ (ThermoFisher Scientific, Invitrogen) in a dilution of 1:2,000 in dilution buffer for 1 h at RT on a rocker ...
• IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion

  Steffen Klein, et al., bioRxiv - Molecular Biology 2023

  Quote: ... cells were incubated with the secondary antibody ‘goat anti-Rabbit Alexa Fluor™ 633’ (ThermoFisher Scientific, Invitrogen) in a dilution of 1:2,000 in dilution buffer for 1 h at RT on a rocker ...
• High-resolution structure of a mercury cross-linked ZIP metal transporter reveals delicate motions and metal relay for regulated zinc transport

  Tuo Zhang, Yao Zhang, Dexin Sui, Jian Hu, bioRxiv - Biochemistry 2023

  Quote: ... the membranes were incubated with a mouse anti-HA antibody at 1:5000 dilution (Invitrogen, Catalog# 26183) at 4°C overnight ...
• Methods Optimization for the Expression and Purification of Human Calcium Calmodulin-Dependent Protein Kinase II Alpha

  Scott C. Bolton, et al., bioRxiv - Biochemistry 2023

  Quote: ... CaMKII phosphorylation was verified by Western blot staining with 22B1 anti-phospho-CaMKII antibody (Invitrogen MA1-047).
• Paternal methamphetamine exposure induces higher sensitivity to methamphetamine in male offspring through driving ADRB1 on CaMKII-positive neurons in mPFC

  Yanyan Zheng, et al., bioRxiv - Neuroscience 2023

  Quote: ... Alexa Fluor 488-labeled donkey anti-mouse secondary antibody (1:500, RRID: AB_141607, Thermo Fisher Scientific, USA). In detail of Figure 1k-l ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Secondary antibodies used were Anti-rabbit IgG Fab2 Alexa Fluor ® 488 (1:20000, 4412S, Molecular probes), were Anti-rabbit IgG Fab2 Alexa Fluor ®594 (1:20000 ...
• Targeting hypoxia-induced circSTT3A decreases breast cancer stem cell formation via degradation of PGK1 protein and serine synthesis

  Ming Xu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... then incubated with appropriate secondary antibodies [Alexa Fluor Plus 555-conjugated anti-mouse (Thermo Fisher scientific, USA) or Alexa Fluor 488-conjugated anti-rabbit (Thermo Fisherscientific ...
• TRiPPing the sensors: The osmosensing pathway of Polycystin 2

  K.M. Márquez-Nogueras, et al., bioRxiv - Physiology 2023

  Quote: ... followed by the appropriate secondary antibody (Alexa Fluor 488 donkey anti-mouse IgG (1:1000, A21202, Invitrogen), Alexa Fluor 546 donkey anti-rabbit IgG (1:800 ...
• Enhancer evolution as a driving force for lineage-specific paralog usage in the central nervous system

  Chika Fujimori, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... After secondary antibody treatment (Alexa Fluor™ 555 conjugated anti-mouse IgG, (1/800; Thermo Fisher Scientific) and anti-rabbit IgG ...
• A central helical hairpin in SPD-5 enables centrosome strength and assembly

  Manolo U. Rios, et al., bioRxiv - Cell Biology 2023

  Quote: ... Secondary Antibodies (1:50,000): HRP conjugated Goat anti-Rabbit IgG (1mg/mL) (Invitrogen, # 65-6120, Lot # J276300).
• Two Ascending Thermosensory Pathways from the Lateral Parabrachial Nucleus That Mediate Behavioral and Autonomous Thermoregulation

  Takaki Yahiro, Naoya Kataoka, Kazuhiro Nakamura, bioRxiv - Neuroscience 2023

  Quote: ... sections were incubated overnight with an anti-RFP rabbit antibody (0.5 μg/ml; R10367, Thermo Fisher Scientific) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000) ...