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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 10 ng total RNA was first reverse transcribed to cDNA using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, UK). TaqMan microRNA assays and TaqMan 2X Universal PCR Master Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... RNA concentration was measured by nanodrop and 500ng to 1000ng total RNAs were used for reverse transcription in 10 ul reaction volume by High-Capacity RNA-to-cDNA kit (ThermoFisher). Equal amount of RNAs were used in the same experiment.
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bioRxiv - Biochemistry 2020Quote: All transcription reactions with magnetic separation were performed using 10 μl of Dynabeads MyOne Streptavidin C1 beads (Invitrogen, Waltham, MA) per 25 μl transcription volume ...
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bioRxiv - Microbiology 2021Quote: ... A reverse transcription (RT) mixture containing the following was added (10 μL reaction volume): 1x SuperScript III (SSIII) FS reaction buffer (Invitrogen), 5 mM dithiothreitol (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... 10 mCi/mL) were generated from these templates using the MAXIscript® T7 In Vitro Transcription Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... cDNA was obtained from 10 μg of RNA following the supplieŕs instruction of the High-Capacity cDNA reverse transcription kit (Applied Biosystems). The used oligonucleotides (Table S1 ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA of EV miRNA was synthesized from 10 ng total RNA using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) and primers specific for mmu-miR-147-3p (alias ...
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bioRxiv - Cancer Biology 2023Quote: 5 µL of purified RNA from serum or conditioned media samples or 10 ng of total RNA from tissue or cells was reversed transcribed using the TaqMan MicroRNA Reverse Transcription Kit (ThermoFisher) and the target-specific RT primer from the cel-miR39-3p (ID 000200) ...
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bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
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bioRxiv - Genetics 2021Quote: ... In vitro transcription of 5’ capped mRNAs was performed using the mMACHINE SP6 Transcription Kit (Ambion) following manufacturer’s protocol and as previously described in (33 ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription into cDNA was performed using the High-Capacity cDNA Reverse Transcription kit (ThermoFisher, 4368813). The manufacturer’s instructions for both kits were followed ...
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bioRxiv - Cell Biology 2020Quote: Reverse transcription was performed on 2μg RNA with the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and subjected to in vitro transcription using the MEGAscript T7 Transcription kit (Thermo Fisher Scientific, AM1334) to obtain the gRNAs ...
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bioRxiv - Developmental Biology 2021Quote: ... gRNA was generated by in vitro transcription using a T7 MEGAscript transcription kit (AM1334-Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and amplified by in vitro transcription overnight using the MEGAscript T7 transcription kit (ThermoFisher Scientific, #AMB1334). Following a second round of clean up ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription for sgRNAs/crRNAs were done using MegaScript T7 Transcription kit (Thermo Fisher Scientific) using a T7 promoter containing template as substrates ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription was performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster City, CA). VH and VL cDNAs were amplified using the ProFlex PCR system (Thermo Fisher Scientific and a previously reported set of degenerate signal peptide (SP ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of RNA was used for reverse transcription (High Capacity cDNA Reverse Transcription Kit, ThermoFisher). Quantitative PCR detecting Gfi1b and Actb was carried out using SYBR green master mix (Bio-Rad) ...
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bioRxiv - Genetics 2019Quote: ... In vitro transcription of the cloned gRNAs was performed using MEGAscript T7 Transcription Kit (Life Technologies). The amplicon was purified with a PCR purification kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... and subjected to in vitro transcription using an mMESSAGE mMACHINE T7 Transcription Kit (Thermo Fisher #AM1344) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by reverse transcription using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). Jade1/2/3 mRNA was assessed by SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Reverse transcription of purified RNA was obtained by RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific). The quantification of gene transcripts was performed by quantitative PCR (Q-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... The reverse transcription reaction was performed using High-Capacity cDNA Reverse Transcription Kit from Thermo Fisher Scientific following the manufacturer’s instructions using random primers ...
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bioRxiv - Microbiology 2022Quote: ... for subsequent in vitro transcription of nsp4 RNA using mMESSAGE mMACHINE T7 Transcription Kit (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... RNA reverse transcription was carried out using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems), following the manufacturer instructions starting from 500 ng of RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... The GRE and GFP templates for in vitro transcription (TranscriptAid T7 High Yield Transcription Kit, ThermoFisher) were purified PCR products made with oligonucleotides containing the T7 RNA polymerase promoter sequence at the 5’ end and the aptameric tag at the 3’ end (Table S1) ...
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bioRxiv - Cell Biology 2022Quote: ... Purified PCR products were subjected to in vitro transcription (MEGAscript T3/T7 Transcription Kit; Life Technologies). Reaction products were digested with TURBO DNase at 37°C for 15 min and purified (MEGAclear Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... before reverse transcription with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA, USA). Quantitative real-time PCR was performed with Blue S’Green qPCR 2X mix (Biozym Scientific GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... followed by reverse transcription using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA). Quantitative RT-PCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription into cDNA was performed using the High-Capacity cDNA Reverse Transcription kit (ThermoFisher, 4368813). The manufacturer’s instructions for both kits were followed ...
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bioRxiv - Biophysics 2022Quote: ... The gRNAs were synthesized by in vitro transcription using the MEGAshortscript T7 Transcription kit (Thermo Fisher) according to the manufacturer’s conditions ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription was performed using the Taqman® Reverse Transcription kit (Life Technologies/ABI, Rotkreuz, CH). Real-time PCR was performed using presynthesized Taqman®-based Assays-on-Demand (Life Technologies/ABI ...
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bioRxiv - Molecular Biology 2023Quote: ... sgRNAs were generated by in vitro transcription using TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific) and cleaned using the Agencourt RNAClean XP Kit (Beckman-Coulter ...
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bioRxiv - Biophysics 2023Quote: ... Reverse transcription (RT) was performed using a High-Capacity cDNA Reverse Transcription kit (4375222, Applied Biosystems) followed by polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... reverse transcription was performed using 0.2ug of RNA and TaqMan MicroRNA Reverse Transcription Kit (ThermoFisher, 4311235), followed by qPCR using TaqMan Universal PCR Master Mix (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... The reverse transcription to cDNA was performed with the Reverse Transcription Reagents (Applied Biosystems, Waltham USA) using random hexamer primer ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription was performed according to manufacturer instructions (High-Capacity cDNA Reverse Transcription kit, Applied Biosystems), and Taqman probes were utilized to detect differential expression using StepOne Plus and QuantStudio3 RT-PCR system ...
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bioRxiv - Cell Biology 2023Quote: Reverse transcription was carried out utilising High Capacity cDNA Reverse Transcription Kit (4368814, Thermo Fisher Scientific). Gene expression was measured by RT-qPCR using the customised primers (500nmol/L each ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed using a High-Capacity cDNA reverse transcription kit (Applied Biosystems, Warrington, WA) with random priming ...
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bioRxiv - Immunology 2023Quote: ... In vitro transcription was performed at 34°C using a T7 MEGAscript T7 Transcription kit (Invitrogen). RNA was purified via lithium-chloride precipitation ...
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bioRxiv - Cancer Biology 2023Quote: 1ug of RNA was used for reverse transcription using TaqMan™ Reverse Transcription Reagents (Invitrogen, #N8080234) and random hexamers ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng of total RNA underwent reverse transcription using the RevertAid RT Reverse Transcription Kit (ThermoFisher) after treatment with DNase (ThermoFisher) ...
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bioRxiv - Systems Biology 2024Quote: ... Pool RNA was produced by runoff T7 transcription using the MEGAshortscript T7 transcription kit (ThermoFisher AM1354) using the purified DNA template ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription was performed using the MEGAscript™ T7 Transcription Kit (Invitrogen, AMB 1334-5) by incubating the reaction at 37 ⁰C for 14 hrs ...
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bioRxiv - Biophysics 2023Quote: RNA was synthesized by in vitro transcription (TranscriptAid T7 High Yield Transcription Kit, Thermo Scientific #K0441), then purified by column purification (RNA Clean & Concentrator ...
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bioRxiv - Biophysics 2023Quote: ... RNA was synthesized by in vitro transcription (TranscriptAid T7 High Yield Transcription Kit, Thermo Scientific #K0441), then purified by column purification (RNA Clean & Concentrator Kits ...
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bioRxiv - Genetics 2024Quote: ... Prior to the reverse transcription by using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems), RNA concentration and sample quality were assessed on a Nanodrop spectrophotometer (Peqlab) ...
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bioRxiv - Bioengineering 2024Quote: ... Reverse transcription was performed utilizing a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat # 4368814) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... Reverse transcription with SuperScript IV (Invitrogen) was performed using a TruSeq R1 RT primer ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 μl 10x transcription buffer (Ambion), 2 μl 10x Dig labeling mix (Roche ...