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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Recombinant Human LCP2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Acquired DDA .raw files were searched against the human UniProtKB/SwissProt database (v2012_07) using Byonic (Protein Metrics) embedded in Proteome Discoverer (ThermoFisher Scientific) to obtain peptide sequence information ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Immunology 2020Quote: ... Mass spectrometry derived data was searched against Human RefSeq 81 protein database in Proteome Discoverer 2.1 (Thermo Scientific, Bremen, Germany) using SEQUEST and Mascot (version 2.5.1 ...
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bioRxiv - Biochemistry 2020Quote: ... serum reactivity to the Spike protein was measured using an HRP-conjugated goat anti-human IgG (H+L) (Invitrogen, 31410) at a 1:3000 dilution for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... 50 and 35µg of proteins (for cell lysates, mouse brains and human cortices, respectively) were added with 1x Sample buffer (Thermo Fisher) and 1x Sample Reducing Agent (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The low and high molecular weight membranes proteins were incubated with respective primary antibodies (Rabbit anti-human BAIAP2L1, PA5-54000, Invitrogen) or (Goat anti-mouse GAPDH ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Organoid culture supernatants and lysates were analyzed for secreted proteins using single- and multiplexed Human ProcartaPlex™ Panel (Thermofisher Scientific), following previously documented procedures.37,38 The plates were read using the Luminex MAGPIX xMAP instrument and xPONENT® software ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish Kcnc3a channel was labeled using a polyclonal antibody originally generated against the human Kcnc3 protein (ThermoFisher Scientific PA5-53714) at a concentration of 2.5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant poly-histidine tagged ectodomains of the SARS-CoV-2 S proteins (614D and 614G) were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using HisTrap FF column (GE Life Sciences)18 ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Genomics 2022Quote: PBMC culture supernatants were analyzed for secreted proteins with Immune Monitoring 65- Plex Human ProcartaPlex Panel (Thermo Fisher Scientific, Carlsbad, CA) as per manufacturer’s instructions (all proteins assayed shown in Table S5) ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 pmol of desthiobiotinylated HOXC13-AS was mixed with magnetic beads and incubated with 200 µg of protein lysate from human adult epidermal keratinocytes (ThermoFisher Scientific). Poly(A)25 RNA ...
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... with RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen cat# 10777-019).
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bioRxiv - Cell Biology 2021Quote: ... RNaseOUT recombinant ribonuclease inhibitor (200 U/mL; Thermo Fisher Scientific) and IGEPAL® CA-630 (0.5 % ...
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bioRxiv - Immunology 2021Quote: ... and recombinant epidermal growth factor (EGF, 2 ng/ml, Gibco).
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bioRxiv - Biochemistry 2020Quote: ... 40 U RNase inhibitor (RNaseOUT ™ Recombinant Ribonuclease Inhibitor, Invitrogen), and various amount of DNA sample (see figures) ...
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bioRxiv - Biophysics 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Neuroscience 2019Quote: ... 200 units/ml RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; Thermofisher), and 1X cOmplete™ ...
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bioRxiv - Cell Biology 2021Quote: ... and 40 U/μL of Recombinant Ribonuclease Inhibitor RNaseOUT (Invitrogen) at 25°C for 2 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin reagent (Life Technologies). After a three-day incubation period ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant monoclonal cystatin A antibodies (Thermo Fisher Scientific, MA5-29200) were immobilized on the silica surface of the microtoroid through EDC/sulfo-NHS covalent coupling ...
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bioRxiv - Developmental Biology 2022Quote: ... we created recombinant pCRTM4-TopoTM plasmids (Invitrogen, Carlsbad, CA, USA) containing 18S and 28S ribosome RNA PCR amplicons ...
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bioRxiv - Biochemistry 2022Quote: RNAseOUT™ recombinant ribonuclease inhibitor (ThermoFisher Scientific, cat. no. 10777019)
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bioRxiv - Microbiology 2020Quote: ... AcMNPV recombinant bacmids were constructed with the bMON14272 bacmid (Invitrogen) and maintained in Escherichia coli strain DH10B (Invitrogen) ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 μL of RNaseOut™ Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant SRPK1 and PKR were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant clones were selected with 120 μg/ml Hygromycin (Invitrogen) and pooled to obtain a stable population maintained in medium containing Puromycin ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... 200 U/mL RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher, 10777019), 400U/mL RNasin (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/mL recombinant bFGF (Thermo Fisher Scientific, PHG0261). Human iPSCs were converted to EPS cells referred to protocol described previously16 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Biophysics 2020Quote: The recombinant tubulin was expressed in HighFive cells (Life Technologies) and purified by three steps of column chromatography (DEAE Sepharose ion exchange chromatography ...