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Citations for Thermo Fisher :
751 - 800 of 8180 citations for Recombinant Human CD3D Flag and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... The secondary antibodies tagged with Alexa Fluor 647 (Invitrogen) at 5 μg/mL were added to the slices and incubated for 1 h before three-times washing and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... or Alexa647-tagged antisera (1:500; Invitrogen, Karlsruhe, Germany) in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... tagged with Alexa Fluor 594 or 488 dyes (Invitrogen), and the signal enzymatically augmented with sequential tyramide signal amplification (TSA amplification kits ...
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bioRxiv - Physiology 2020Quote: ... Amp-3 and HRP-tagged probe (Thermo Fisher Inc), one drop each for 30 ...
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bioRxiv - Immunology 2021Quote: ... and Rhodamine or Alexa Fluor 488-tagged Phalloidin (Invitrogen) were used for counterstaining cells ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies and detected using fluorescently tagged secondary antibodies (Invitrogen, goat raised anti-mouse 488 and Anti-rabbit 555) ...
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bioRxiv - Molecular Biology 2023Quote: ... Whi5 tagged with GFP was obtained from Thermo Fisher Scientific GFP yeast clone collection ...
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bioRxiv - Biophysics 2023Quote: Full-length human MRS2 (Uniprot Q9HD23) conjugated with a FLAG tag at the C-terminus was expressed in Expi293F cells (Thermo Fisher Scientific, A14527). Cells at a density of 2.0-3.0 × 106 cells/ml were transfected with the MRS2-encoding pCMV plasmid using polyethylenimine (PEI ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... FLAG epitope was detected by mouse anti-FLAG M2 primary (Invitrogen, 1:1000 dilution) and goat anti-mouse IgG HRP secondary (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Immunology 2021Quote: Hela cells stably-expressing EGFP-tagged NLRP3 and mApple-tagged RAB5A were seeded into Nunc™ Lab-Tek™ 8-well Chambered Coverglass (155411, ThermoFisher Scientific) on the day before experiments ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Synthetic Biology 2021Quote: ... anti-FLAG (Invitrogen, cat# 740001), anti-V5 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-Flag (Thermo Fisher, A36797) and eluted by 4× lituium dodecyl sulfate (LDS ...
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bioRxiv - Microbiology 2022Quote: ... anti-FLAG (Thermo Fisher Scientific), or anti-HA (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG-HRP (ThermoFisher Scientific) was used at 1:1,000 dilution using 2% dry milk in TBST ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... rat anti-FLAG (ThermoFisher Scientific) was used as the secondary antibody ...
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bioRxiv - Biophysics 2022Quote: ... and rabbit anti-FLAG (Invitrogen). Secondary antibodies conjugated to DyLight 800 or 680 (Li-Cor ...
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bioRxiv - Immunology 2024Quote: ... anti-Flag (Invitrogen, MA1-91878),anti-RIPK1 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
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bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Molecular Biology 2019Quote: 2.5D-Fc and 2.5F-Fc fusions were labeled with the succinimidyl ester derivative of Alexa Fluor 488 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... washed with FC buffer and resuspended in FC buffer including viability die and analyzed on Attune (Thermofisher Scientific). The permeabilized cells were excluded from the analysis due to intracellular staining.
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bioRxiv - Bioengineering 2023Quote: ... Bound SiRPα-Fc was detected with a secondary biotinylated rabbit anti-IgG Fc monoclonal antibody (Thermo Fisher Scientific) followed by HRP-conjugated streptavidin (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... Flag-CHCHD10 S59L or Flag alone were transfected in HeLa cells using lipofectamine 2000 (Invitrogen). Twenty-four hours post transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 30% heat-inactivated FCS (Gibco) and penicillin-streptomycin in each well ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% heat-inactivated FCS (Gibco) and penicillin-streptomycin and 30 ng/mL Doxycycline for 10 days prior to experiments.
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bioRxiv - Immunology 2021Quote: ... Fc block (anti-CD16/CD32, 101302 Affymetrix) was included to minimize nonspecific antibody binding ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented with 10% FCS (Gibco 10270-106), 100 U/ml LIF (homemade) ...
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bioRxiv - Immunology 2021Quote: ... and 10% heat-inactivated (FCS; Thermofisher, Scientifix). Cultures were supplemented with 10IU IL-2 (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... FCS and antibiotics were purchased from Invitrogen/Thermofisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10 % vol/vol FCS (Gibco). Splenocytes were mashed through a 100 μm cell strainer (Falcon) ...
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bioRxiv - Cell Biology 2021Quote: ... 10% Fetal Calf Serum (FCS, Life Technologies), and 100 μg/mL Primocin (Invivogen ...