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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Rat ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The entire rat SELENOP 3’UTR was cloned into the pcDNA3.1 vector using the TOPO-TA cloning kit (Invitrogen). 3 copies of the viral RNA MS2 sequence separated by random sequence were synthesized commercially from IDT technologies (Table 1) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the amygdala of adult rats using the MagMAX mirVana Total RNA Isolation kit (ThermoFisher). RNA quality was assessed using the Qubit RNA BR Assay (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution, Molecular Probes - cat. A21210) and 2 μg/mL of DAPI ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006], Goat anti-Rabbit 594 [Invitrogen, A11037], Goat anti-Mouse [Invitrogen, A11029]) diluted in blocking solution was added to the tissues and incubated for 3 hours at room temperature ...
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bioRxiv - Genetics 2021Quote: ... for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by secondary antibody coupled to 10-nm gold (British Biocell or Bbisolutions) and secondary fluorescence antibodies (donkey-anti-rat Alexa594 cat# A-21209, Molecular Probes Europe B.V. ...
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bioRxiv - Molecular Biology 2019Quote: ... and then for 1 h at room temperature in 2 µg/mL Alexa 488-conjugated donkey anti-rat antibody (A-21208, Thermo Fisher). Slides were mounted with ProLong Gold containing DAPI (P36931 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were fixed and stained with anti-rat or anti-mouse secondary antibody conjugated to Alexa 568 (1:200, A11077, Thermo Fisher) or Alexa 647 (1:800 ...
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bioRxiv - Plant Biology 2020Quote: ... The seedlings were incubated and gently shaken in the dark at RT in 500 μL of a 1/10,000 (v/v) dilution of secondary antibody (anti-rat-Alexa Fluor 488; Thermo Fisher Scientific) in PBS for 90 min ...
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bioRxiv - Microbiology 2020Quote: ... and 50% FBS followed by 2 h incubation with primary antibodies and a subsequent incubation with a suitable secondary antibodies in PBS for 45 min (anti-rabbit Alexa Fluor 488 and anti-rat 568; Molecular Probes) and DAPI in PBS for 15 min (Life technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Slides were then washed 3 times in 1% BSA/1xPBS and were incubated in 1:1000 dilution (in 1% BSA/1xPBS) of the following secondary antibodies: Alexa488-conjugated goat anti-rat (Molecular Probes) and Alexa647-conjugated goat anti-rabbit (Molecular Probes ...
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bioRxiv - Pathology 2021Quote: ... tissues were washed in PBS and probed using a goat-anti-rat IgG 488 secondary antibody (1:500, Invitrogen A-11006) for one hour ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoblotting of A-ZIP53 the blocked blot was incubated with the 1:5000 dilution of HRP labeled rat anti-T7 antibody (Thermo Scientific) at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution, Molecular probes) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... RAW 264.7 cells were stimulated with the ID8 cell-derived CM supplemented with 5 μg/mL of IL-18 neutralizing antibody (R&D, Minneapolis, MN, USA) or 5 μg/mL of rat IgG1 Isotype as a control (Thermo-Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in D-PBSTB and incubated for 1hour 30 min at room temperature with secondary antibodies – donkey anti-rat Alexa 555 (dilution 1:1000; Molecular Probes) and donkey anti-rabbit Alexa 647 (dilution 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then washed three times for 15 minutes in 0.1 % Tween-20 in PBS and incubated in PEMBALG containing monoclonal anti-rat antibody conjugated with Alexa488 (1:1000 dilution, Life Technologies) for one hour ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed with PBS-S-T and incubated for 1h at 37°C with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed three times for 5 min with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Staining was performed for 1 hour with primary antibodies at the following dilutions: rat mouse anti-SAG1 (1:1000, Thermo Scientific), rabbit anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed and incubated for 60 min at room temperature with an appropriate secondary antibody: anti-rat (1:500, Thermo Fisher Scientific Cat# A-11006 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor 594–conjugated anti–rat IgG and Alexa Fluor 488–conjugated anti–mouse IgG)(Molecular Probes).
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bioRxiv - Developmental Biology 2019Quote: ... NeoMarkers) and anti-Laminin (4H8-2, MilliporeSigma) antibodies followed by anti-rabbit Alex-488 and anti-rat Alexa-568 (Molecular Probes) were used for detection of FLT1 expression in capillaries ...
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bioRxiv - Biochemistry 2019Quote: ... all used at 1:10,000 dilution in TBS with 0.1% v/v Tween-20 (TBS-T) and horseradish peroxidase-conjugated rat IgG secondary antibody (#31470, Thermo Fisher Scientific) used at 1:10,000 dilution in 5% non-fat dry milk dissolved in TBS-T.
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bioRxiv - Pathology 2020Quote: ... cryoslides were incubated for 2 hours at room temperature with secondary antibodies (AlexaFluor 488-, 555-, 647-, or Cy3-conjugated anti-rabbit, rat, mouse, goat IgG) (1:400, Life Technologies, Waltham ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation was performed for 30-60 minutes in room temperature with anti-rat Alexa 555 (1:400, Invitrogen, A21434). Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... they were subsequently stained for 30 minutes with appropriate secondary antibodies 1000× diluted in PBS (Goat anti-Rat AF488 (Invitrogen, A11006), Goat-anti-Rabbit AF488 (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... the sections were further incubated with Alexa647-conjugated donkey antibody to goat IgG (for rats injected with Alexa647-conjugated CTb) or Alexa488-conjugated donkey antibody to goat IgG (10 μg/ml; A11055, Thermo Fisher) (for rats injected with Alexa488-conjugated CTb ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were mixed with either 0.8 μg/mL of rat anti HA antibody and 30 μL of pre-washed dynabeads protein G (Thermo Fisher; 10003D) or 40ul of pre-washed anti-Flag M2 affinity gel (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Immunology 2022Quote: ... samples were washed with PBTX for three times and replaced with secondary antibody (1%, goat anti-mouse Alexa Fluor 488 and goat anti-rat Alexa Fluor 568, ThermoFisher UK) and 1 ug/ml DAPI solution ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed for 5 minutes 3 times with PBS followed by incubation with goat anti-Rat IgG (H+L) Alexa Fluor 594 secondary antibody (A11012, Thermo Fisher) at 1:500 dilution for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with secondary antibodies of goat-anti-rat 488 and goat-anti-rabbit 594 (1:500, Invitrogen, A11006, A11037) 1.5 hours in room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Wings were then incubated in 1:500 secondary antibody at the concentration 1:500 with anti-rat AF488 (Invitrogen, #A-11006) and anti-Guinea pig AF555 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... followed by probing with secondary antibodies (goat anti-rabbit Alexafluor 488, A11034, 1:500; goat anti-rat Alexafluor 647, A21247, 1:500, ThermoFisher Scientific) applied and washed as per the primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
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bioRxiv - Microbiology 2023Quote: ... the coverslips were subjected to staining using the following secondary antibodies: goat anti-rat Alexa Fluor 594 (Thermo Scientific, #A-11007), goat anti-mouse Alexa Fluor 594 (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... blocked again and stained with secondary antibody (Goat-Anti-Rat conjugated to Cy3 AP136C, SigmaAldrich) and DAPI (D9542, SigmaAldrich) and then mounted with vectashield (1551231, Fisher Scientific,).
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in DPBS for 10 min and incubated with secondary antibodies (Donkey anti-rabbit Life Technologies cat. no A31573, 1:400; Donkey anti-rat ThermoFisher Scientific cat ...
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eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... the slices were incubated with secondary antibodies (Goat anti-rat IgG Alexa Fluor 568, A2110844; Goat anti-rabbit IgG Alexa Fluor 568, A2155282; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Plant Biology 2024Quote: ... secondary Alexa Fluor 488 antibodies (diluted 1:200 in 0.1% BSA in PBS, goat Anti-Rat-IgM, Thermo Fisher Scientific, Denmark) were added and the sections were incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and 568 conjugated goat anti-rabbit or anti-rat IgG antibodies (1:500 each; Invitrogen; Carlsbad, CA, USA) were used for fluorescent signal detection ...
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bioRxiv - Cancer Biology 2024Quote: ... The following secondary antibodies and dilutions were used: goat anti-rat Alexa Fluor 488 1:1000 (Thermo Fisher Scientific, A-11006), goat anti-rat Alexa Fluor 594 1:1000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by the secondary antibody Alexa Fluor 647 goat anti-rat (1:500, Cat# A-21247; Thermo Fisher Scientific, RRID: AB_141778). Later ...