Labshake search
Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... mice (4 WT; Table 1A) were cardiac perfused with AlexaFluor 488 conjugated wheat germ agglutinin (Thermofisher Scientific) at 15ug/mL in 20mL of phosphate buffered saline (PBS) ...
-
bioRxiv - Neuroscience 2022Quote: ... blood was collected by either cardiac puncture using K3EDTA blood collection tubes (Fisher Scientific, 22-040-035) immediately before perfusion ...
-
bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used for staining: mouse anti-cardiac isoform of cTNT (ThermoFisher Scientific, 1:200), anti-WT1 (Abcam 1:100) ...
-
bioRxiv - Neuroscience 2020Quote: ... cells in primary neurospheres were trypsinised with trypsin-EDTA (Invitrogen), dissociated mechanically ...
-
bioRxiv - Cell Biology 2020Quote: Primary cell culture performed in DMEM (ThermoFisher, Waltham MA, USA) with fibronectin-depleted (depleted via column purification using gelatin sepharose ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary isolated PBMCs were Cell Tracker Green (CTG) (Thermo Fisher) labelled at 4×106 cells/mL for 30 minutes at 37°C 5% CO2 ...
-
bioRxiv - Pathology 2022Quote: ... they were cultured as primary cells in KnockOut DMEM (Invitrogen) supplemented with 10% v/v Knockout Serum Replacement (KSR ...
-
bioRxiv - Cell Biology 2022Quote: ... All primary cells were cultured in MEM (Thermo Fisher Scientific) supplemented with 1 mM pyruvate (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... the cells were incubated with primary antibodies (Thermo Fisher Scientific), including rabbit anti-OCT4 ...
-
bioRxiv - Immunology 2023Quote: ... Primary immune cells were kept in RPMI1640 medium (Life technologies) supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were transfected with 1.5 μg human α-synuclein-GFP plasmid DNA [48] using lipofectamine-LTX (Thermofisher) on DIV6 and imaged on DIV10 using an inverted ZEISS Z1 Cell Observer fitted with a Colibri2 cool LED 5-channel fast wavelength switching system ...
-
bioRxiv - Cell Biology 2021Quote: Young adult primary human skeletal muscle (HSKM) progenitors isolated from a 20-year old female subject’s quadriceps muscles (Gibco) were seeded onto plates coated with 0.1% gelatin solution (Merck-Millipore) ...
-
bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; Thermo Fisher Scientific) and the human HCC cell line HuH7 (ATCC ...
-
bioRxiv - Genomics 2021Quote: ... RNA for gene expression analysis from primary human pseudoislets was extracted using the PicoPure RNA isolation kit (Life Technologies) and cDNA was synthesized using the Maxima first strand cDNA synthesis kit (Thermo Scientific) ...
-
bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
-
bioRxiv - Cell Biology 2023Quote: ... C2C12 myotubes and biopsy- derived human primary myotubes were transfected with plasmids using lipofectamine 3000 (Thermofisher, Waltham, MA, USA) following the supplier’s instructions.
-
bioRxiv - Systems Biology 2023Quote: ... Thawed Primary human hepatocytes were recovered in Cryopreserved Hepatocyte Recovery Medium (Cat. CM700, Thermo Fisher Scientific, Waltham, MA, USA), and re-suspended in ice-cold HBSS prior to surgery ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary human neutrophils were used directly out of thaw and cultured on poly-l-lysine (15 µg/mL, Thermofisher) coated tissue culture dishes ...
-
bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted to analyze the gene expression in each of the porcine organs (liver, lung, spleen, heart, kidney, testis, and ovary) and cultured porcine fibroblast cells using Trizol reagent (Invitrogen). Complementary DNAs (cDNAs ...
-
bioRxiv - Cell Biology 2021Quote: ... fibroblasts were trypsinized and 3.5×105 cells were resuspended in 100 µL of R buffer (Life Technologies, Carlsbad, CA, USA). Subsequently ...
-
bioRxiv - Cell Biology 2021Quote: Protein was isolated from NPCs or MRC5 fibroblasts by lifting the cells from the plate with trypsin (Invitrogen #15400-054) and lysing the cells in nuclear lysis buffer (10 mM NaCl ...
-
bioRxiv - Bioengineering 2022Quote: Gene editing of pig fibroblast cells were performed by electroporation of wildtype large white pig fibroblast cells with a plasmid encoding Piggybac transposase and pBv1-EF-6X plasmid using Neon Transfection System (Invitrogen) under condition of 1450 V ...
-
bioRxiv - Genomics 2022Quote: ... followed by seeding 5×104/cm2 of mouse embryonic fibroblast (MEF) cells (CellBiolabs, CBA-310) in MEF medium (DMEM [Gibco, 11965118] ...
-
bioRxiv - Molecular Biology 2021Quote: ... KH2 embryonic stem cells were cultured in gelatin-coated plates in presence of irradiated DR4 Mouse Embryonic Fibroblasts (ThermoFisher #A34966), and maintained in Knock-Out DMEM (GIBCO ...
-
bioRxiv - Immunology 2020Quote: ... Mouse NIH-3T3 fibroblast cells were obtained from (ATCC® CRL-1658™, Manassas, VA) cultured in DMEM (Life Technologies), supplemented with 2 mM of L-glutamine ...
-
bioRxiv - Cell Biology 2022Quote: ... HeLaEGFP-AID-Sororin (a gift from Dr. Daniel Gerlich56) cells and mouse embryonic fibroblasts were cultured in DMEM high glucose (Gibco) supplemented with 10% Tet-approved FBS ...
-
bioRxiv - Systems Biology 2019Quote: ... for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960) and with serum+LIF media composing of ...
-
bioRxiv - Microbiology 2021Quote: Baby hamster kidney fibroblasts (BHK-21J) and African green monkey kidney (Vero E6) cells were maintained in Minimum Essential Medium (Gibco) supplemented with 10% fetal bovine serum (HyClone) ...
-
bioRxiv - Cell Biology 2022Quote: ... Human male diploid lung WI-26 SV40 fibroblasts (WI-26 cells; #CCL-95.1, ATCC; RRID: CVCL_2758) were cultured in DMEM/F12 GlutaMAX medium (#31331093, Thermo Fisher Scientific), containing 10% FBS ...
-
bioRxiv - Genomics 2022Quote: Mouse ES cells were cultured on irradiated mouse embryonic fibroblasts (MEFs) under standard conditions: 500 ml DMEM (Gibco, 41965-039), 20% fetal bovine serum (Biological Industries ...
-
bioRxiv - Molecular Biology 2023Quote: ... Individual blastocysts were then deposited into wells containing Mitomycin C-inactivated mouse embryonic fibroblasts as feeder cells and cultured in KnockOut DMEM (Gibco) supplemented with 20% Knockout Serum Replacement (Gibco) ...
-
bioRxiv - Systems Biology 2023Quote: ... the monocytic cell line THP-1 (ATCC TIB-202) and immortalized foreskin fibroblast BJ cell line (CRL-2522) were cultured in RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2023Quote: NIH/3T3 embryonic mouse fibroblast cells (ATCC, CRL-1658) were cultured at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2023Quote: ... BJ-5ta cells (ATCC CRL-4001) and MRC-5 fibroblasts (ATCC CCL-171) were grown in Dulbecco modified Eagle medium (DMEM, Gibco) supplemented with 10 % (v/v ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10,000 TdTomato+ and TdTomato-cells of the fibroblast enriched fractions were sorted directly into Tri reagent® (TR118, Fisher Scientific), and RNA was extracted according to manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells) expressing CD40-ligand in DMEM F12 media (Gibco), the B cells were immortalized by retroviral transduction with BcL6 ...
-
bioRxiv - Immunology 2020Quote: ... Human embryonic kidney (HEK293T) cells and HeLa cells were cultured in DMEM (Gibco) supplemented with 100U/ml Penicillin (Sigma-Aldrich),100μg/ml Streptomycin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Cells were activated with Dynabeads human T cell activator CD3/CD28 beads (Gibco) for 2 h and 48 h.
-
bioRxiv - Immunology 2020Quote: ... Human polyclonal NK cells were isolated by NK cell negative isolation kits (Invitrogen) from peripheral blood or from normal donor buffy coats obtained from the blood bank ...
-
bioRxiv - Microbiology 2021Quote: BEAS-2B (Human Normal Lung Epithelial Cells) cells were cultured in DMEM (Gibco) containing 10% FBS (Gibco ...
-
bioRxiv - Immunology 2023Quote: Mouse primary T cells were cultured in T cell medium (TCM): RPMI1640 medium (Gibco) supplemented with 5% fetal bovine serum (FBS) ...
-
bioRxiv - Immunology 2023Quote: Mouse primary T cells were cultured in T cell medium (TCM): RPMI1640 medium (Gibco) supplemented with 5% fetal bovine serum (FBS) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The patient-derived fibroblast cell line 4950T-F and the LUSC cell line 2427T (14) were cultivated in DMEM/HAMs F12 (Thermo Fisher Scientific, Waltham, Massachusetts, US) with 10 % fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells harvested on different days of cardiac differentiation were stained with cTnT antibody (Troponin T, mouse monoclonal antibody, Thermo Fisher Scientific, MS-295-P, 1:200) and then with donkey anti-mouse IgG (H+L ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
-
The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
-
bioRxiv - Cancer Biology 2019Quote: NIH3T3 fibroblasts were maintained in Dulbecco‘s modified Eagle‘s medium (DMEM) (Invitrogen) supplemented with 10% calf serum ...
-
bioRxiv - Neuroscience 2019Quote: ... Fibroblast cultures were expanded and passaged using trypsin (Thermo Fisher Scientific) prior to being frozen or sent for reprogramming ...