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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Mouse ATPAF2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... BHK-21 cells were transfected 24 h prior to pseudotype inoculation with either empty plasmid or ACE2 expression plasmid (0.1 μg/well) using Lipofectamine LTX (Thermo Fisher Scientific). In order to study the antiviral activity of Camostat mesylate ...
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bioRxiv - Cell Biology 2019Quote: Stable cell lines were generated by co-transfecting the plasmid of interest together with a plasmid carrying the resistance gene for hygromycin with Cellfectin II (Life Technologies), and the selection process was started 2 d after transfection by supplementing the media with 250 μg/ml Hygromycin B.
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bioRxiv - Developmental Biology 2021Quote: ... a transposase expressing plasmid and a TOL2 plasmid expressing mCherry (as a transfection control) following the Lipofectamine 2000 protocol (Life Technologies). 48 hours post transfection ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral vector system includes the pLEX-MCS plasmid and the PMD2G and psPAX2 packaging plasmids (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Genetics 2020Quote: ... HEK293T cells were seeded on 48-well plates and transfected with SlugABEmax/SlugBE4max plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM(Gibco ...
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bioRxiv - Genetics 2020Quote: ... cells were seeded on 48-well plates and transfected with Cas9 plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: The inducible XIST constructs within the HT1080 2-3-0.5a cells were modified by transiently transfecting two unique gRNA plasmids (pSPgRNA) and a Cas9 plasmid providing transient puromycin resistance (pSpCas9(BB)-2A-Puro) using Lipofectamine 3000 (ThermoFisher #L3000008). The day before the transfection the HT1080 cells were split into 24 well plates ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were transfected with Fsp I linearized CD40L plasmid (a kind gift from G. Freeman21,22) and Pvu I linearized pcDNA3-Neomycin plasmid) using Lipofectamine 2000 Reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with sleeping beauty transposon plasmid pSBi-Hyg-BFP (hTMEM120A cDNA) and sleeping beauty transposase plasmid pCMV (CAT) T7-SB100 with Lipofectamine2000 (Life Technologies). Three days after transfection ...
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bioRxiv - Genetics 2022Quote: ... Plasmids and packaging plasmids psPAX2 and pMD.2G were co-transfected into cells using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000015). The medium was changed after 12 hour incubation ...
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bioRxiv - Neuroscience 2022Quote: ... and plasmids were purified 18 h after the inoculation with a 96-well plasmid purification kit (PureLink Pro, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T and HCT116 cells were plated at a density of 20,000 cells per well in a 96-well plate and transfected with 200 ng of Cas13 expression plasmid and 200 ng of gRNA expression plasmid using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol DMEM (4.5 g/L glucose ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were plated at a density of 200,000 cells per well in a 24-well plate and transfected with 800 ng of dCasRx expression plasmid and 800 ng of gRNA expression plasmid using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol in HEK media without penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Molecular Biology 2023Quote: Extrachromosomal arrays were introduced by injection of 10 ng/μl plasmid DNA purified using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) into the gonad of 1 DOA IR1284 (zcIs14[myo-3p::mtGFP] ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were co-transfected with plasmids encoding GFP-nsP3 fusions and the integrase-encoding plasmid using Lipofectamine™ 3000 according to the manufacturer’s recommendations (ThermoFisher Scientific). Clones of blasticidin-resistant ...
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bioRxiv - Genomics 2023Quote: ... cells at 80–90% confluency were co-transfected with 0.25 µg of single-sgRNA (or 4sgRNA) plasmids and 0.25 µg of dCas9-VPR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific). Three days post-transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 ml of the culture was pelleted by centrifugation and plasmid DNA was isolated using a GeneJET Plasmid Miniprep Kit (Thermo Scientific). The plasmid sequence was verified using BigDye 3.1 sequencing chemistry and an ABI3130 Genetic Analyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in Opti-MEM (Thermo Fisher # 31985062). For 96-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... co- transfected with lentiviral packaging plasmids (pCEP4-tat, pHEF-VSVG and pNHP) and lentiviral expression plasmids using Lipofectamine 2000 (Invitrogen, 11668019) as previously described50 with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: SOX2:GFP ESCs were plated into a gelatinised 6-well plates in SL and lipofected with the linearized Gata6:mCherry-Hygromycin plasmid and the CRISPR plasmid using Lipofectamine2000 (ThermoFisher 11668019) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmid using the Lipofectamine LTX and Plus Reagent (Invitrogen, 15338-100) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmids using the Lipofectamine LTX and Plus Reagent (Invitrogen #15338-100). Transfected cells were first selected by 1 μg/ml puromycin for 2 days and subsequently selected by 250 μg/ml hygromycin for 6 days ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral packaging plasmids (pCMV-D8.9 and pCMV-VSVG) and reporter plasmids were transfected into HEK-293T cells using Lipofectamine 3000 (ThermoFisher cat#L3000001). Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232) ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... HD patient iPSC-derived astrocytes were transiently transfected with 2 μg hFAN1 plasmid and control plasmid (pTT5 vector) by Lipofectamine 3000 (Invitrogen; L3000008) every week ...
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bioRxiv - Immunology 2024Quote: ... pMDLg/p.RRE (18 μg) packaging plasmids and 15 μg of expression plasmids were mixed and transfected into 293T cells using Lipofectamine 3000 (Invitrogen #L3000150) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The HEK293T packaging cells were transfected with OSA-expressing plasmids with lentiviral packaging plasmids using Lipofectamine Reagent 2000(Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... transfections were performed by introducing 20 μg of targeting plasmid and 60 μg of linearised repair template (purified using Pure-Link™ HiPure Plasmid Midiprep Kit, Invitrogen) into ∽108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza) ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg dual sgRNA plasmids or 1 μg sgRNA plasmids and 1 μg homologous recombination donor using Neon™ NxT Electroporation System (Invitrogen). After 48 hr ...
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bioRxiv - Immunology 2024Quote: ... 18 μg of pMDLg/p.RRE Gag/Pol plasmid and 18 μg of pRSV.Rev plasmid alongside 15 μg of transfer vector plasmid encoding for CAR of interest using Lipofectamine 2000 (Thermo Fisher Scientific) and Opti-MEM (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µg of each plasmid (gagpol, vsv-g, and transfer plasmid) were combined with 2 ml Opti-MEM I Reduced Serum Medium (Gibco 31985070) and 90 µl MirusBio TransIT-LT1 Transfection Reagent (Mirus MIR2306 ...
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bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were co-transfected with 200 ng pCAGEN plasmid for TDP-43 and 1000 ng pCAGEN plasmid for the other protein using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000001) in 12-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-V5 (Invitrogen) 1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Por1 (Invitrogen), and rabbit anti-Sec61 (generous gift from Martin Spiess ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Pgk1 (Invitrogen), mouse anti-Por1 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse (Invitrogen 35518) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-GFP (Invitrogen) and anti-HA (Biolegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... AF594-anti-mouse (Invitrogen). Small molecular compounds such as 2-BP (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-V5 (Invitrogen), STAM (ProteinTech) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-CD79a (ThermoFisher); mouse anti-CD4 (AbD Serotec) ...
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bioRxiv - Cancer Biology 2020Quote: ... and mouse αGFP (Invitrogen) at appropriate dilutions.
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bioRxiv - Cell Biology 2021Quote: ... and mouse laminin (Thermofisher). Within 24–30 h of initial dissociation neurons were pelleted by centrifugation (5 min ...
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bioRxiv - Microbiology 2021Quote: ... Donkey anti-mouse (Invitrogen) and Donkey anti-rat (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... mouse Ub-K63 (ThermoFisher) or rabbit anti-GST (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-RetP1 (ThermoFisher) (1:100) ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-Prdx5 (Invitrogen), rabbit anti-hnRNPK (CST) ...