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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... using Human Taqman probes (Thermo Fisher Scientific) for ...
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bioRxiv - Neuroscience 2023Quote: ... ELISA for human Aβ42 (Thermo Fisher; KHB3441) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid DNA was purified from miniprep cultures using a GeneJET plasmid DNA miniprep kit (Thermo Fisher, K0502). Constructs were checked with a control digestion performed using the same restriction enzymes used for ligation (BamHI and EcoRI) ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid DNA was extracted from these cultures using a GeneJET plasmid miniprep kit (Thermo Scientific, Dublin, Ireland) and sent to GATC (Konstanz ...
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bioRxiv - Cell Biology 2021Quote: ... The donor plasmid and the pX260 plasmid were co-transfected into ES cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The plasmid was isolated from transformed clones using the GeneJET Plasmid Isolation Kit (Thermo Scientific Inc, USA). Plasmid sequencing was outsourced to Bioserve Technologies Pvt ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection of horse PepT1 plasmid or empty plasmid was performed with 293fectin transfection reagent (Invitrogen/Thermo Fisher) as per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection of horse PepT1 plasmid or empty plasmid was performed with 293fectin transfection reagent (Invitrogen/Thermo Fisher) as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... or ABEmax)-encoding plasmid (0.7 μg) and each sgRNA expression plasmid(0.3 μg) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The injected DNA plasmids were prepared using PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Invitrogen, # A31231), aliquoted based on concentrations ...
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bioRxiv - Microbiology 2021Quote: ... This plasmid is derived from plasmid pMV001 that has been synthesized with GeneArt Gene Synthesis (ThermoFisher Scientific). pMV001 carries the Pada-ada-alkB operon where codons encoding Ada C38 and C321 have been replaced to encode A38 and A321 ...
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bioRxiv - Plant Biology 2019Quote: ... amplified from wild-type Col-0 cDNA downstream of the GFP coding sequence in the pB7WGF2 plasmid (Ghent plasmids collection, http://bccm.belspo.be/index.php) via Gateway technology (Invitrogen). The same was done for the p35S::GFP-S2Lb construct ...
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bioRxiv - Biochemistry 2019Quote: ... Colonies were scraped from the plate and plasmid DNA isolated with GeneJET Plasmid Miniprep Kit (Thermo Scientific). The isolated plasmid DNA was used to transform E ...
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bioRxiv - Systems Biology 2021Quote: ... 10 ng of each entry plasmid and destination plasmid was mixed with Gateway LR clonase II (Invitrogen) in a 4 μL volume and incubated at 25 °C for at least 16 hours ...
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bioRxiv - Systems Biology 2021Quote: ... 10 ng each of entry plasmid and destination plasmid was mixed with Gateway LR clonase II (Invitrogen) in a 2 μL final volume and incubated at 25 °C for at least 16 hours ...
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bioRxiv - Genetics 2021Quote: Plasmids were purified using the PureLink Fast Low-endotoxin Maxi Plasmid Purification kit (ThermoFisher Scientific, Cat. #A35895). All plasmids were sequenced prior to injection ...
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bioRxiv - Genetics 2020Quote: Plasmids were purified using the PureLink Fast Low-endotoxin Maxi Plasmid Purification kit (ThermoFisher Scientific, Cat. #A35895). All constructs were fully re-sequenced prior to injection ...
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bioRxiv - Immunology 2021Quote: ... Sequence verified plasmid encoding for mCD22-Fc was transfected together with Flp recombinase-expressing plasmid pOG44 (Invitrogen) using lipofectamine LTX (Invitrogen ...
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bioRxiv - Immunology 2022Quote: All the plasmids for analytical expression were purified using PureLink™ HiPure Plasmid Miniprep Kit (#K210003, Invitrogen). Plasmid vectors were transiently transfected into HEK293 FreeStyle cells using polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids were isolated from an overnight culture of single colonies using GeneJET Plasmid Miniprep Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid purification was carried out using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... and a control plasmid (pEGFP-C1 plasmid) using the Lipofectamine™ 3000 Transfection Reagent (ThermoFisher Scientific, USA) as instructed in the protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... we co-transfected 600 ng reporter plasmid and 200 ng PhiC31 integrase plasmid using Lipofectamine 2000 (Invitrogen). After selection by geneticin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were isolated from controls with a Purelink HQ Mini Plasmid Purification Kit (Invitrogen, Cat #K2100-01). Bacteria that were to be desiccated were concentrated by centrifugation (5,000 rpm ...
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bioRxiv - Systems Biology 2023Quote: ... Positive candidates were selected for plasmid DNA isolation using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The construction pDRF1-GW ymTq2Δ11 was confirmed by DNA sequencing (https://www.macrogen-europe.com/) ...
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bioRxiv - Microbiology 2023Quote: ... was used for routine cloning and plasmids were isolated using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). All the kits were used following the manufacturers’ protocol.
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA was isolated from the bacteria using PureLink HiPure Plasmid Filter Miniprep Kit (Thermo Fisher Scientific) and pooled to produce the final plasmid library.
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR assays were performed using TaqMan Gene Expression Master Mix and the probes human NEMO/IKBKG (Hs00415849_m1) and human GAPDH (Hs03929097_g1) (ThermoFisher Scientific). Amplifications were run in a Bio-Rad CFX96 real-time PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... The second incubation were performed with secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch; Anti-Human IgM-Cy5, Invitrogen) diluted in ratio 1:1000 in incubation buffer with 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: Human monocytic U937 cells (ATCC) and human embryonic kidney 293T cells (ATCC) were cultured in RPMI 1640 medium (Gibco), supplemented with 5-10% fetal calf serum (BioWest) ...
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bioRxiv - Cancer Biology 2021Quote: Human HCC cell lines (Huh7, HepG2) and Human HeLa were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Hep-3B human hepatocellular carcinoma cells and MCF7 human mammary carcinoma cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Gibco) supplemented with 10% heat-inactivated and charcoal stripped fetal calf serum and antibiotics ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... The plasmids were co-transfected with packaging plasmids (pspAX2 and pMD2G) into 293T cells using Turbofect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were sequenced and transfected along with lentiviral packaging plasmids into HEK293FT cells with Lipofectamine 3000 (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The cloned plasmids were then isolated from their bacterial cultures using GeneJET Plasmid Miniprep Kit from Thermo Fisher scientific (catalog number-K0503).
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bioRxiv - Immunology 2021Quote: ... Plasmid of positive clones were extracted using the GeneJET Plasmid Miniprep Kit following the manufacture protocol (ThermoFisher scientific). and tThe nucleocapsid cloning was confirmed by digestion with using BamHI and NheI FastDigest enzymes (ThermoFisher Scientific) ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Plasmids were extracted with Favorprep Plasmid Extraction Mini Kit and cleaved with FastDigest SacI restriction enzyme (Thermo Scientific) to eliminate the toxin plasmids ...