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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for ATP citrate lyase Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Nucleotide substrates were purchased from: ATP (Adenosine 5′-triphosphate Disodium Salt Trihydrate, Fisher Scientific BP413-25) GTP (Guanosine 5′-triphosphate sodium salt hydrate ≥95% ...
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bioRxiv - Genetics 2019Quote: ... the oligonucleotide probes were 5’ end labeled with [γ-32P]ATP using polynucleotide kinase (Thermo Scientific), purified through Sephadex G-50 chromatography ...
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bioRxiv - Biophysics 2021Quote: ATP hydrolysis was analysed in steady state reactions using an ENZCheck Phosphate Assay Kit (Life Technologies). 150 μL samples containing standard reaction buffer supplemented with 2 mM of ATP were assayed in a 18 BMG Labtech PherAstar FS plate reader at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... ATP was normalized to protein content measured with the Pierce BCA protein assay kit (ThermoFisher Scientific). Triplicate technical replicates were performed for each sample ...
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bioRxiv - Systems Biology 2021Quote: ... Probes (10 pmol each) were labeled with [γ-32-P]-ATP using T4 PNK (Thermo Fisher) and hybridized to the membrane one by one in hybridization buffer (4× Denhardts solution ...
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bioRxiv - Microbiology 2019Quote: Hydrolysis of ATP by HSP70 was measured using an EnzChek phosphate assay kit (Thermo Fisher Scientific). All reaction mixtures contained 50 mM ATP ...
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bioRxiv - Cell Biology 2022Quote: ... Purified small RNAs were labeled with 32P-γ-ATP using T4 polynucleotide kinase (Thermo Fisher Scientific). After electrophoretic separation by 15% urea-containing denaturing polyacrylamide gel in ×0.5 TBE ...
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bioRxiv - Immunology 2022Quote: ... NAD or ATP-treated CD4 cells (as described above) were resuspended in annexin-binding buffer (ThermoFisher) and incubated with 1µl 100µg/ml Propidium Iodide (PI ...
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bioRxiv - Developmental Biology 2022Quote: ... The probes (10 pmol each) were radiolabelled with [γ-32P]ATP using T4 PNK (Thermo Scientific) and hybridized to the membrane overnight in hybridization buffer (4× Denhardt’s solution ...
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bioRxiv - Synthetic Biology 2023Quote: ... A luciferin-luciferase luminescent reporter assay was then used to quantify the intracellular ATP concentration (Invitrogen). OD600 measurements were also made at each time-point and used to calculate an average intracellular ATP concentration with 1 OD600 as 109 cells/mL and an average cell volume as 1fL62,63.
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotide probes were radiolabelled with γ-32P-ATP (Hartmann Analytic) using T4 polynucleotide kinase (Thermo Scientific), purified using illustra MicroSpin G-25 columns (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with anti-SARS-CoV2 nucleocapsid mouse mAb (MA5-29981, Invitrogen; 1:1000) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The 1A5 and 3F5 mAbs were conjugated with Alexa Fluor 647 (AF647) (Thermo Fisher Scientific) for flow cytometry ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660; 1:5000; Invitrogen), ACE2 antibody (rabbit monoclonal ab108252 ...
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bioRxiv - Immunology 2021Quote: ... 836.31 and 0526.02) used 4-fold excess PGT151 mAb and 1:60 α-hIgG:PE (Invitrogen) pre-incubated for 60 minutes as the phycoerythrin signal ...
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bioRxiv - Immunology 2020Quote: ... purified mAbs were buffer exchanged into PBS using Zeba™ Spin Desalting Plates (ThermoFisher Scientific) and stored at 4°C until use.
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bioRxiv - Immunology 2020Quote: ... purified mAbs were buffer-exchanged into PBS using Zeba Spin Desalting Plates (Thermo Fisher Scientific) and stored at 4°C until use.
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-glial fibrillary acidic protein (GFAP) Mab (ThermoFisher Scientific #13-0300, 1:500). Sections were then washed in PBST and incubated with fluorophore-conjugated secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated M2e-MAb was separated from non-reacted material by dialysis (10kd MWCO; ThermoFisher Scientific) in 1X phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were placed in 250 ml freshly made antigen retrieval solution (0.1 M sodium citrate buffer pH 6.0, Fisher Scientific) and pressure cooked (A ...
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bioRxiv - Genetics 2021Quote: ... Cells were then sonicated for 10 sec at 20% power and 0.5 mL of citrate buffer containing 2 μM of SYTOX green (Invitrogen) was added to the suspension ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were incubated in a 10 mM sodium citrate buffer with 0.05 % Tween 20 (pH of 6) (Fisher Scientific) for 15 minutes at 99□ prior to fixation ...
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bioRxiv - Neuroscience 2020Quote: ... where sections were heated to 100°C for 20 min in citrate buffer (TA-250-PM1X; Thermo Scientific, UK). After cooling in dH2O ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were contrasted with 0.4% lead citrate for 1 min and imaged in a Tecnai T12 transmission electron microscope (ThermoFisher), operated at 100 kV and equipped with an axial 2k CCD camera (TVIPS).
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bioRxiv - Immunology 2022Quote: Blood was collected in collection tubes containing anticoagulant acid citrate dextrose (ADC) Solution B (Fisher Scientific, Hampton, NH, USA). Ficoll (Stemcell Technologies ...
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bioRxiv - Genomics 2021Quote: ... Cell aggregates were then dissociated by sonication and 40 μL of cell suspension were incubated with 170 μL of 50 mM sodium citrate buffer containing 0.5 μM Sytox Green (Invitrogen). Data were acquired on a MASCQuant Analyzer (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat-induced antigen retrieval was performed by incubating slides in a solution of 10 mM sodium citrate pH 6 (MilliporeSigma; SX0445-20) with 0.05% (v/v) Tween20 (ThermoFisher) at 98C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and re-fixed for 10 minutes before being briefly rinsed with wash buffer (2x saline-sodium citrate [SSC, Invitrogen, AM9770] ...
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bioRxiv - Cell Biology 2021Quote: ... was modified by adding the following reagents to the stated final concentrations: 1mM trisodium citrate dihydrate (ThermoFisher Scientific, BP327), 91.2mM sodium chloride (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Ultrathin sections were stained with uranyl acetate and lead citrate and analysed with a Tecnai Spirit (Thermo Fisher Scientific) operated at 120 kV.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were pelleted at 3,000 g, aspirated, resuspended in SYTOX buffer (500 µL 50mM sodium citrate [pH 7], 1 µM SYTOX green (Invitrogen)) ...
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bioRxiv - Biophysics 2023Quote: ... Fresh human blood was drawn into a citrated tube (3.8% sodium citrate, citrate:blood = 1:9) and mixed with MitoTracker Deep Red (Invitrogen, USA). Citrate chelates extracellular calcium ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μl of each sample were mixed with 170 μl of 50 mM Na-citrate and 0.5 μM Sytox Green (S7020, ThermoFisher). Prior to the FACS analysis ...
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bioRxiv - Biophysics 2023Quote: ... containing 10,000 units/mL of penicillin 10,000μg/mL of streptomycin in a 10 mM citrate buffer; Fisher Scientific Ltd). Cells were kept at 37°C and 5% CO2 as standard and were used 2 days after passage.
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bioRxiv - Developmental Biology 2023Quote: ... Grids were stained with lead citrate (Merk) and examined with a Tecnai T12 transmission electron microscope (Thermo Fisher Scientific). Digital electron micrographs were acquired with a bottom-mounted TVIPS TemCam-XF416 4k × 4k CMOS camera.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant gene was cloned into pFastBac1 expression vector (Life Technologies) for bacmid production ...
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bioRxiv - Cell Biology 2019Quote: Recombinant Bax was labeled by NBD dye (IANBD amide, Life Technologies) as reported previously (Kale et al. ...
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bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2.5 ng/mL of recombinant human IL-2 (Thermofisher, PHC0027). T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher ...
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bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...