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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 6 Methoxy 2 methyl 1H benz de isoquinoline 1 3 2H dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... ToPro-3 (1:1000, Invitrogen). Secondary antibodies used in this study were purchased from Invitrogen and include ...
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bioRxiv - Cell Biology 2019Quote: ... BODIPY-493/503 Methyl Bromide (used at 1/1000 from 1 mg/ml ethanol stock) and CellTracker green (C2925) from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Mix 1 contains 3 siRNAs (CliniSciences, CRH7929) and Mix 2 contains two siRNAs (ThermoFisher Scientific, 1299001 and 4392420). As a negative control ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer containing 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies). A BD FACSAria III Cell Sorter (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Cell Biology 2020Quote: ... Eggs were washed extensively in D-PBSTB and incubated with secondary antibodies for 2h at RT - donkey anti-rabbit Alexa 555 (dilution 1:1000; Molecular Probes), goat anti-rat Alexa 488 (dilution 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated for 2h with secondary antibodies conjugated to either AlexaFluor 488 or 594 (1:400, Life Technologies) and then washed again ...
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bioRxiv - Cell Biology 2023Quote: ... were serum-starved in FBS-free DMEM for 2h and then were incubated with 1µg.µL-1 of transferrin-conjugated to Alexa-Fluor-647 (Tf-647, Life Technologies, T-23366) for 10 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... After 3×10 min washes in PBS slices were incubated for 2h at RT in PBS containing a secondary antibody (donkey anti-goat Alexa Fluor Plus 647, 1:500, A21447, Invitrogen, UK). During a final round of washes tissue slices were counterstained with DAPI (NucBlue™ Fixed Cell ReadyProbes™ Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then rinsed in PBS and incubated for 2h at room temperature with secondary antibodies: Alexa488 donkey anti-guinea pig (1:500, ThermoFisher, A11073), Alexa488 goat anti-chicken (1:500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Genomics 2022Quote: ... 3 μM CHIR99021 (Stemgent) and 0.5× N-2 Supplement (Gibco)) which was changed daily for the cells ...
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bioRxiv - Neuroscience 2022Quote: ... with passaging every 2-3 days with trypsin/EDTA (Gibco). 3 biological replicates were generated for proteomic analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... and were purified 2-3 times by Trizol LS (Ambion) to completely remove the carry over plasmid DNA template ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 3 mins at 90C and separated on a 6% denaturing PAGE gel (Invitrogen). The RNA was then transferred to a HyBond-N+ nylon membrane (GE Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 wells of a 6-well plate of mESC (corresponding to 6*10^6 cells approximately) were resuspended in 1 mL Trizol (Invitrogen,15596018) vortexed two times for 30 seconds and incubated 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were washed 3 times with PBS and incubated for 1h at room temperature with goat anti-rabbit AlexaFluor-568 (ThermoFisher, Invitrogen A-1101, 5ug/ml working solution). After washes with PBS ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Cell Biology 2021Quote: ... 338-AC, 100 ng/mL) in DE medium (RPMI 1640, 1% Penicillin/Streptomycin, 1% L-Glutamine (Gibco, ref. 25-030-081). DE differentiation was induced by threating MES differentiated cultures for 2 days with ACTIVIN A (R&D ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... were used as a model for peritoneal mesothelial cells and cultured as described previously (De La Garza, 2012, Liu et al., 2009) and grown in MCDB 131.Medium 199 (1:1 - Gibco, NY) with 15% FBS ...
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bioRxiv - Genetics 2021Quote: ... and a NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA). Only total RNA samples with a RNA integrity number value >8 were used for the preparation and construction of PacBio or HiSeq sequencing libraries.
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bioRxiv - Cancer Biology 2019Quote: ... The Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) was used to evaluate the quality and purity of RNA ...
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bioRxiv - Neuroscience 2020Quote: ... tested using the Nanodrop D1000 (Thermo Scientific, Wilmington, DE), indicating samples were of good purity ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... following the standard protocol (ThermoFisher Scientific, Wilmington, DE, USA). The final library pool was quantified using the Qubit dsDNA HS assay (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Sf9 cells were obtained from Invitrogen (Life Technologies, DE) and grown at 27°C in monolayer culture with Grace’s medium (Sigma /Life Technologies TM ...
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bioRxiv - Physiology 2023Quote: ... using murine TaqMan assays (Thermo Fisher Scientific, Wilmington, DE) to measure p16Ink4a (Cdkn2a ...
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bioRxiv - Genetics 2023Quote: ... CA)] and a NanoDrop spectrophotometer [RRID:SCR_018042 (ThermoFisher Scientific, Wilmington, DE)] ...
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bioRxiv - Cell Biology 2023Quote: ... Amplification Grade (Thermo Fisher Scientific Inc., Wilmington, DE, USA) and retrotranscribed to cDNA with SuperScript III RNase Transcriptase (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... quantified on a qubit fluorometer (Thermo Scientific, Wilmington, DE), and visualized on an Agilent 4200 Tapestation (Agilent ...
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bioRxiv - Physiology 2023Quote: ... Total RNA was quantified (Nanodrop 3300, Thermofisher, Wilmington, DE) followed by bioanalysis (Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... The NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and the Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
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bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
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bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
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bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...