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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 6 Isopropyl 2 methylbenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... slides were stained with 1:10,000 DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific; Cat. No.: D3571) for 10 min at room temperature before coverslips were applied with PBS and glycerol (1:1 ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... ATs and TTs were fixed in 4% PFA and stained with 4′,6- diamidino-2-phenylindole (DAPI) (Invitrogen) to identify cell nuclei ...
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bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed in FACS buffer and then stained with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher) or 7AAD (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... the cell nucleus was stained with 1:1000 DAPI (4’,6’-diamidino-2-phenylindole; Invitrogen; Catalog No: D1306) nuclear stain for 10 minutes at RT ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Bioengineering 2020Quote: ... and a porous polyester membrane (AR Brown-US) were cut using a laser cutter (Epilog Laser, CO) and cleaned with 70% isopropyl alcohol (Fisher Scientific). The porous polyester membrane had pore size of 0.4 μm ...
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bioRxiv - Microbiology 2021Quote: ... Induction of genes on plasmids was accomplished by the addition of 0.5 mM Isopropyl β- D-1-thiogalactopyranoside (IPTG) (Thermo Fisher), 0.2% arabinose (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in 1 L liquid LB medium to an OD600nm of 0.6-0.7 before addition of 1 mM isopropyl-β-D-1-thiolgalactopyranoside (ITPG; Thermo Fisher Scientific) to induce expression ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein expression was induced by the addition of 50 μM Isopropyl β-D-1-thiogalactopyranoside (Fisher Scientific, Fair Lawn, NJ USA) to bacterial cell cultures for 2.5 hours with shaking at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated on ice for 15 min and induction was initiated with the addition of 0.4 mM isopropyl ≥-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Proteins were produced for 16-18 hr at 18°C.
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 [1-301] fragment N-terminally fused to a 6xHis tag was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-10N N-terminally fused to MBP was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 full length N-terminally fused to GST was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Genetics 2024Quote: ... RNA was precipitated by mixing with isopropyl alcohol (0.2 mL) as well as 1 uL of GlycoBlue Coprecipitant (AM9515, Thermo Fisher Scientific) and samples were incubated at −80°C overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were mounted on ProLong Gold with 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Delta-Vision II microscope system with an Olympus IX-71 inverted microscope using a 100x objective A ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted in ProLong™ Gold Antifade Mountant containing 4’,6’-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). Images were acquired at 100x magnification using a Nikon Eclipse Ti fluorescence microscope fitted with a Hamamatsu C11440 digital camera ...
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bioRxiv - Genomics 2020Quote: ... 2 µl cDNA (diluted 1:20) was used in a 6 µl Fast SYBR Green qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates ...
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bioRxiv - Biochemistry 2019Quote: ... fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene) and ANS (1-anilinonaphthalene-8-sulfonic acid) were also from Invitrogen; PMB ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Cancer Biology 2020Quote: ... Proton transport was measured using ATP-dependent quenching of 9-amino-6-chloro-2-methoxy-acridine (Acridine Orange, ThermoFisher) fluorescence quenching for isolated vacuoles as previously described21
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed twice with PBS and stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Scientific #D1306) diluted 1:200 in 0.5% BSA in PBS ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were mounted with the ProLong™ Diamond Antifade Mountant with 4’,6-Diamidino-2-Phenylindole (DAPI) (ThermoFisher Scientific). Ki-67 signals were visualized with Nikon microscopy ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were affixed using Prolong Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, Cat. # P36935).
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed again in PBS and incubated overnight with 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and Alexa Fluor 647-conjugated βIII tubulin (Abcam ab190575) ...
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bioRxiv - Microbiology 2021Quote: ... Nuclear staining was performed by incubating slides with 4’,6-diamidino-2-phenylindole (DAPI, final 0.5 μg/mL; Invitrogen). Images were captured using an epifluorescence microscope (Nikon Eclipse Ni ...
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Cancer Biology 2022Quote: T98G cells (2 × 105 into 6 well plate) were reverse transfected with siRNAs (Table S3) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Cell nuclei were stained with ProLong™ Diamond Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific). Samples were imaged using a confocal setup (Zeiss Airyscan equipped with a 63x ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were mounted on glass slides in 4′,6-diamidino-2-phenylindole (DAPI) containing Fluoromount-G medium (Thermo Fisher). Images were acquired by an Eclipse A1 laser-scanning microscope ...
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bioRxiv - Bioengineering 2020Quote: We used a mixture of 6 μm and 2 μm diameter fluorescence beads (C16509, Thermo Fisher; F8825 Thermo Fisher) in our experiment ...
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bioRxiv - Bioengineering 2020Quote: We used a mixture of 6 μm and 2 μm diameter fluorescence beads (C16509, Thermo Fisher; F8825 Thermo Fisher) in our experiment ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen) and images were acquired using Nikon A1-R confocal microscope using the NIS Elements software ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed in 0.1% IGEPAL/PBS and mounted using ProLong Gold antifade reagent with 4’-6-Diamidino-2-phenylindole (DAPI) (Invitrogen). Immunofluorescence studies in HeLa cells were carried out as described above ...
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bioRxiv - Cell Biology 2019Quote: ... Mounting medium contained DAPI (4’, 6-diamidino-2-phenylindole) to visualize the nucleus (Molecular Probes Inc., Eugene, OR, USA). Negative controls were performed with either no primary or no secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... incubated 5 minutes with at room temperature with 500 ng/ml 4’,6-Diamidino-2-Phenylindole Dilactate (DAPI; ThermoFisher) in DPBS and mounted in ProLong®Gold (ThermoScientific).
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bioRxiv - Microbiology 2021Quote: ... Dissociated cells and remaining pieces of tissue were placed in a single well of a 6-well plate containing 2 mL of Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 20% heat-inactivated fetal bovine serum (FBS ...