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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Mouse ENKD1 shRNA (nucleotides targeting 403-423 bp, 5′-CGCTCACCCAAGTATGACAAT-3′) were cloned into pLKO.1 (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Molecular Biology 2020Quote: ... while the EASY-Spray C18 column (Thermo Scientific, 15 cm×75 μm ID, 3 μm, 5 μm) was used as the analytical column ...
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bioRxiv - Cancer Biology 2020Quote: ... 5’-\56-FAM\ CCT ACC TTA ACC TCC C-3’ with Minor Groove Binder (MGB; ThermoFisher Scientific). PCR was performed using 10X Master Mix to yield a final volume of 25 µl and final working concentrations of 16.6mM (NH4)2SO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were thawed at room temperature and washed 3 times 5 minutes in DPBS (Gibco, 14190-094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 5′-CCCAAGACTTCCACCTACATTC -3′ (Tm = 62°C) and subsequently subcloned into PCR II-TOPO-TA cloning vector (Invitrogen). Riboprobes were generated with a BIOT-NTP labeling kit (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Primers used to amplify the 5’/3’ junction with Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) are listed in Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (version v1.5.1, Applied Biosystems). Cq values were obtained via automated threshold determination ...
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bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 6-carboxyfluorescein (FAM)-5= CCG TCA ATC AAG GAG CGC CTC 3=-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... 6-carboxyfluorescein (FAM)-5’ CCG TCA ATC AAG GAG CGC CTC 3’-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Microbiology 2020Quote: ... flanked by 5’ Nhel/3’Apal sites for subcloning into the pcDNA3.1(+) vector (Thermo Fisher Scientific, USA). HEK293T cells and Vero E6 cells were purchased from ATCC ...
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Genomics 2022Quote: ... for 3–5 s and then washed thoroughly in phosphate-buffered saline (PBS) (pH 7.4, Gibco, USA). Single zona pellucida-free embryos were carefully pipetted and placed into individual tubes containing 2 μL PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 mM probenecid (Life-Technologies: catalog # P36400) and 5 µM Fluo-3 (Life Technologies; catalog # F-14218). Cells were incubated at 37°C for 30 minutes before being centrifuged and resuspended in modified L-15 media containing 2% FBS and 2.5 mM probenecid ...
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bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
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bioRxiv - Microbiology 2021Quote: ... for 3 hours at 37 °C before transforming 5 μL into TOP 10 chemically-competent bacteria (Invitrogen). Mutations were confirmed in selected clones by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... (RV-5′-GCCGTGGGTACTGAGAAAG-3′) and the fragments were bi-directional sequenced using (3130 Genetic Analyser; Applied Biosystems). Further the results were compared with the reference sequence of ARHGEF40 gene using NCBI-BLAST program and the chromatogram was analysed using Chromas lite (2.1 ...
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bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Immunology 2022Quote: ... Probe: 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-NFQ-MGB-3’ (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2022Quote: ... Sections were washed 3× for 5 min each in PB and mounted with Prolong Gold (Thermo Fisher) and Deckglaser cover glass (Cat#NC1776158) ...
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bioRxiv - Cell Biology 2022Quote: ... 5′-RACE and 3′-RACE were done using the GeneRacer method according to the manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′) and KAPA HiFi HotStart ReadyMix (Thermo Fisher Scientific), cleaned up using RNAClean XP SPRI beads three times ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (ThermoFisher SuperSignal West) and imaged using a ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... At 3 hours post infection (hpi) cells were seeded on 5 mm round glass coverslips (Thermo Scientific) and mounted on a sample holder specially designed for LLSM ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA (5′-TGTGAATCGTAGGAGCGCTG-3′) was synthesized via in vitro GeneArt™ Precision gRNA Synthesis Kit (ThermoFisher, A29377) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and WNK1 (5’ CAGACAGUGCAGUAUUCACTT 3’) were transfected into HDMEC using Lipofectamine RNAiMax reagent (Thermo Fisher Scientific, 13778150). After 48 (TSC22D1 ...
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bioRxiv - Neuroscience 2024Quote: ... A guide RNA targeting exon 41 of the LRRK2 gene (5’-CTCAGTACTGCTGTAGAATG-3’) was commercially synthesized (ThermoFisher) and used to form ribonucleoprotein (RNP ...
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bioRxiv - Cell Biology 2024Quote: ... The 5’ homology arm-Linker-mAID-3’UTR fragment was synthesized using Genestrings® (Thermo Fisher Scientific). The Cas9-NCAPH2-gDNA plasmid and the two template plasmids (BSR or Hygro ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were passaged every 3-5 days at 70-80% confluence using 0.25% Trypsin-EDTA (Life Technologies). Cells were grown at 37°C in 5% CO2.
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bioRxiv - Physiology 2024Quote: ... the cells were resuspended in 5 mL FACS buffer (PBS supplemented with 3% fetal bovine serum [ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Total RNAs for 3 to 5 independent experiments performed in triplicate were extracted by Trizol (Life Technologies) and quantified on NanoDrop ...
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bioRxiv - Molecular Biology 2024Quote: ... Spike-in controls cel-miR-39-3p (C. elegans sequence: 5’-UCACCGGGUGUAAAUCAGCUUG-3’, Invitrogen, Waltham, MA, USA) and cel-miR-54-3p (C ...
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bioRxiv - Biochemistry 2024Quote: ... synthetic genes encoding the fusion proteins flanked by 5’-NdeI and 3’-XhoI sites were synthesized (Invitrogen GeneArt Gene Synthesis ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The 5 ‘and 3 ‘RACE were performed using the FirstChoice RLM-RACE kit (Ambion, Austin, TX, USA), according to Watanabe et al ...
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bioRxiv - Biochemistry 2024Quote: ... REV 5′ - GTG GAT AAC CCC TCC CCC AGC CTA GAC CA - 3′ provided by (Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... and position (1235-1252 bp) 5’ GcCGcATtCTcGAgGTtG 3’ (modified region corresponding to siRNA from Thermo Fisher s38908). The following restriction sites were modified ...
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bioRxiv - Cell Biology 2024Quote: ... and position (2164-2187 bp) 5’ gAgGAcGAgGCcTGGTAtCAaAaa 3’ (modified region corresponding to siRNA from Thermo Fisher s228498). The following restriction sites were modified ...
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bioRxiv - Cell Biology 2024Quote: ... and position (607-627 bp) 5’-cAaGAaTTcCGaGAgACtAAc- 3’ (modified region corresponding to siRNA from Thermo Fisher s34150). The following restriction site was modified ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... CHIKV was labeled with the lipophilic fluorescent probe DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt; Life Technologies), as described previously [5] ...
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bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris and 3-8% Tris-Acetate gels (Life Technologies). Nitrocellulose membranes were developed with horseradish peroxidase (HRP ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and Antibiotic-Antimycotic Mixed Stock Solution (Nacalai) and were passaged every 3-4 days with TryPLE (ThermoFisher Scientific). For microscopy observations ...
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bioRxiv - Bioengineering 2020Quote: ... Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Thermofisher) was diluted in sterile RPMI-1640 (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... a sample of eluates (3%) was separated on acrylamide NuPAGE Novex 4–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.