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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 2 Triisopropylsilyl Oxazole 5 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Molecular Biology 2020Quote: ... 5×107 mouse ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 2 mM DSG (Thermo Scientific) for 45 minutes at 25°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Developmental Biology 2020Quote: ... Probe 2 (mutation; 5’ FAM – CTC CCG CCT GAT T 3’) and Platinum Quantitative PCR SuperMix-UDG w/ROX (Life Technologies). The products were amplified and analysed on an ABI StepOne PCR machine.
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bioRxiv - Microbiology 2020Quote: ... ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 1 ml TRIzol Reagent (Thermo Scientific) using a Tissue Lyser II (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... boiled in reducing sample buffer for 5 min and resolved on 4–12% Bis-Tris Bolt gels and transferred using an iBlot 2 (Thermo Fisher). Blots were blocked in 2.5% milk in 1% TBS-Tween before staining with antibodies (Table S5) ...
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bioRxiv - Cell Biology 2021Quote: EdU (5-ethynyl-2′-deoxyuridine) detection was performed with the Click-iT EdU Alexa Fluor 555 Imaging Kit (Thermo-Fisher Scientific). EdU was diluted in DMSO to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: Brains from 2-5 days old adult flies were dissected in PBS and briefly stored in Schneider’s insect cell medium (Life Technologies, A820) supplemented with 5% heat-inactivated FBS prior to fixation in 4% paraformaldehyde in PBS for 45 min at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Dried pellets were resuspended in 43 µL of water and incubated with 5 µL of 10X TURBO DNase buffer and 2 µL of TURBO DNase (Thermo Fisher) for 1 hour at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... cell pellets were resuspended in 1 mL FACS buffer (DPBS with 2% BSA, Miltenyi Biotec #130-091-376, plus 0.05mM EDTA, ThermoFisher #15575020, and 5 ng/mL SCF, ThermoFisher #PHC2115) and incubated with Zombie Violet Fixable Viability dye (BioLegend #423113 ...
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bioRxiv - Biochemistry 2021Quote: Approximately 20 micrograms of purified 6xHis-tagged KRAS4BA59T were reduced with 5 mM tris(2-carboxyethyl)phosphine (ThermoFisher Scientific, Cat #77720) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... packed with ReproSil- Saphir C18 1.5 μm (Dr. Maisch GmbH, Ammerbuch, Germany) with a 2 hours segmented gradient using an EASY-nLC 1,200 (Thermo Fisher Scientific). LC solvents were A ...
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bioRxiv - Bioengineering 2022Quote: Cell proliferation in T-SLICE zones was analyzed using an EdU (5-ethynyl-2’-deoxyuridine) Cell Proliferation Kit (Click-iT− EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye: Invitrogen). Cell culture media was supplemented throughout with 10 uM EdU ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Plant Biology 2022Quote: ... peptides were pre-concentrated with 0.1% TFA on a trap column (AcclaimR PepMap 100 column, 100 μm i.d. × 2 cm, C18, 5 μm particle size, 100 Å pore size; Thermo Scientific) at a flow rate of 7 μL/min for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... the media was aspirated from the cells and replaced with the Fura2-loading solution (5 µM Fura-2-AM and 0.15% Pluronic F127 (Invitrogen, Waltham, USA) dissolved in D-MEM/Ham’s F-12 with 10 %FBS) ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of sample were loaded onto the trapping column (Acclaim PepMap RSLC, 100 μm × 2 cm, nanoViper, 5 μm 100Å C 18 particles, Thermo Scientific) using 0.1% acetic acid at a flow rate of 7000 nl/min for 3 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with 2× saline sodium citrate (SSC) buffer for 5⍰min and blocked with UltraPure Salmon Sperm DNA (Thermo Fisher) in QuikHyb Hybridization Solution (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Microbiology 2022Quote: ... A 3% agarose gel with 4 μL ethidium bromide was loaded with 5 μL PCR reaction and 2 μL GeneRuler 100bp ladder Plus (Thermo Fisher) and run for 40 minutes at 100 volts ...
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bioRxiv - Systems Biology 2024Quote: ... comprising a Acclaim PepMap 100 trapping column (2 cm x 100 µm i.d., 5 µm 100 Å C18, Thermo Fisher Scientific) and a consecutive nanoEase M/Z Peptide BEH analytical column (25 cm x 75 µm i.d. ...
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bioRxiv - Pathology 2024Quote: ... The samples were loaded onto in Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) and separated on an Acclaim PepMap RSLC (75 μm x 50 cm ...
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei were labelled with a PBS-Hoechst solution at 2 μg/mL for 5 min at RT and samples were covered in mounting medium (ProLong P36930, Thermofisher scientific). Labelled cells were analysed with an inverted LSM 980 confocal microscope with Airyscan 8Y (Carl Zeiss Ltd.) ...
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bioRxiv - Cell Biology 2024Quote: ... Two sets of 12 100 µL 2.66 ng/ µL enriched resuspended mitochondria aliquots in 200 µL PCR tubes were made per condition and heated on a temperature gradient of 42-64°C (2°C steps) for 5 minutes at each temperature using a Veriti 96-well thermal cycler (Applied Biosystems).
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER rolls and unrolled control samples were incubated at 37 °C in complete organoid media with 50 μM of 5-Iodo-2’deoxyuridine (IdU, Fisher Scientific) and 20 uM of Telox64 (courtesy of Mark Nitz ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was co-transcriptionally capped using m7(3’OMeG)(5’)ppp(5’)(2’OMeA)pG capping reagent (Hongene Biotech) in a “one-pot” reaction followed by digestion with DNase I (Thermo Fisher). Linear mRNA was purified using Dynabeads MyOne Carboxylic Acid beads (Thermo Fisher).
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bioRxiv - Immunology 2024Quote: ... The cDNA generated was then diluted 2-5 fold in RNase- and DNase-free water and mixed with PowerUP SYBR Green Master Mix (Applied Biosystems) and primers (listed in Supplemental Table 3 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, ThermoFisher) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amoebae were grown from frozen spores at room temperature on 100 x 15mm plates filled with 30mL SM/5 agar (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Microbiology 2023Quote: ... recipient animals were lightly anesthetized with isoflurane and retro-orbitally injected with 2 to 5 million live donor bone marrow cells resuspended in 50 μL of 1X PBS (Gibco, MA). Donor animals were rested for a minimum of 6 weeks before MHV-A59 infection or up to 11 weeks for assessing Ly6e expression and degree of chimerism.
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bioRxiv - Microbiology 2022Quote: ... The Hela cell line was provided by HeLa CRM-CCL-2™ and maintained at 37°C with 5% CO2 in Dulbecco’s high glucose modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... in a humidified incubator at 37 °C in a 5% CO2 atmosphere in wells containing Neurobasal medium supplemented with 2% B27 (Thermofisher #17504044), 1% N2 (Thermofisher #17502048) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% w/v sucrose-2% w/v agar food supplemented with 2.75% w/v yeast extract (Fisher Scientific, Waltham, MA), 2.75% w/v tryptone (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Orbitrap Eclipse mass spectrometer (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed using RealQ Plus 2× Master Mix Green (Ampliqon, #A324402) on a QuantStudio 5 machine (Applied Biosystems). Expression was calculated using the delta-delta-Ct method ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 µm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were then blocked by incubation in 2% BSA in PBS at RT for 30 min followed by 5% donor bovine serum (ThermoFisher Scientific) for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA was then diluted 20 times and 5 µl of the dilution was mixed with 2×VeriQuest Fast SYBR Green qPCR Master Mix (Affymetrix, 75690) and primers for the real-time qPCR analysis ...