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7901 - 7950 of 10000+ citations for Recombinant Mouse Il1f6 None tagged since 2019

• Muscle-specific Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

  Harriet P. Lo, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-rabbit and anti-mouse Alexa488-and Alexa555-conjugated secondary antibodies (Molecular Probes). Affinity purified rabbit anti-Cavin4 was raised as described previously (Lo et al. ...
• Regulation of GluA1 Phosphorylation by D-amphetamine and Methylphenidate in the Cerebellum

  Laura Cutando, et al., bioRxiv - Neuroscience 2020

  Quote: ... goat Cy5-coupled anti-mouse (1:500, Thermo Fisher Scientific Cat#A-10524), goat Cy3-coupled anti-mouse (1:500 ...
• Simple methods for quantifying super-resolved cortical actin

  Evelyn Garlick, et al., bioRxiv - Cell Biology 2021

  Quote: ... incubation with the secondary antibody (goat anti-mouse Alexa Fluor 488, A11001, Invitrogen) proceeded for a further hour.
• A cell-based platform for oxidative stress monitoring in motor neurons using genetically encoded biosensors of H₂O₂

  Elizaveta I. Ustyantseva, et al., bioRxiv - Cell Biology 2021

  Quote: IPSCs were maintained onto mitotically inactivated mouse embryonic fibroblasts in KnockOut DMEM (Gibco) with 15% knockout serum replacement (Gibco) ...
• A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission

  Aurélia C Balestra, et al., bioRxiv - Microbiology 2020

  Quote: ... and secondary antibody Alexa 488 conjugated anti-mouse IgG (Invitrogen-A11004; 1:1000). The slides were then mounted in Vectashield 19 with DAPI (Vector Labs ...
• SARS-CoV-2 infection leads to acute infection with dynamic cellular and inflammatory flux in the lung that varies across nonhuman primate species

  Dhiraj Kumar Singh, et al., bioRxiv - Immunology 2020

  Quote: ... mouse CD68 (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C) for macrophages and pDC’s were identified by co-staining of PE conjugated mouse anti-human CD123 (BD Biosciences ...
• Over-expression Screen of Interferon-Stimulated Genes Identifies RARRES3 as a Restrictor of Toxoplasma gondii Infection

  Nicholas Rinkenberger, et al., bioRxiv - Microbiology 2021

  Quote: ... and probed with 1:1000 goat anti-mouse Alexa Fluor 488 (Life Technologies) and 1:1000 goat anti-rabbit Alexa Fluor 568 (Life Technologies ...
• HelR is a helicase-like protein that protects RNA polymerase from rifamycin antibiotics

  Matthew D. Surette, et al., bioRxiv - Biochemistry 2021

  Quote: ... or with a 1:5000 dilution of mouse α-RpoB (8RB13, Fisher Scientific) followed by a 1:20000 rabbit α-mouse HRP conjugate (Ab97046 ...
• High-Throughput Developability Assays Enable Library-Scale Identification of Producible Protein Scaffold Variants

  Alexander W. Golinski, et al., bioRxiv - Bioengineering 2020

  Quote: ... and 1 μg of a goat-anti-mouse AlexaFluor647 antibody (A-21235, Invitrogen) and incubated at 4 °C for 20 min while protected from light ...
• Accessible, Open-source Hardware and Process Designs for 3D Bioprinting and Culturing Channels Lined with iPSC-derived Vascular Endothelial Cells

  Andrew R Gross, Roberta S. Santos, Dhruv Sareen, bioRxiv - Bioengineering 2021

  Quote: ... and then stained with secondary antibodies AlexaFluor 568 anti-mouse (Fisher Scientific A10037) or AlexaFluor 568 anti-rabbit (Fisher Scientific A10042 ...
• Tetrahymena thermophila Granule Lattice Protein 3 Improves Solubility of Sexual Stage Malaria Antigens Expressed in Escherichia coli

  Cengiz Akkale, et al., bioRxiv - Bioengineering 2021

  Quote: ... Blocking buffer was refreshed and mouse anti-His antibody (Thermofisher, Cat#MA1-21315) was added at a dilution factor of 1:2000 in blocking buffer ...
• Unraveling Endothelial Cell Phenotypic Regulation By Spatial Hemodynamic Flows With Microfluidics

  Sarvesh Varma, et al., bioRxiv - Bioengineering 2020

  Quote: ... Secondary antibodies include: Alexa Fluor 555 donkey anti-mouse IgG (A31570, Life Technologies), Alexa Fluor 488 donkey anti-rabbit IgG (A21206 ...
• PD-1-induced T cell exhaustion is controlled by a Drp1-dependent mechanism

  Luca Simula, et al., bioRxiv - Immunology 2021

  Quote: ... and anti-mouse Alexa Fluor 488-coniugated secondary antibody (1:500, Life Technologies). Slides were analysed under a Zeiss Axioscope A1 and microphotographs were collected using a Zeiss Axiocam 503 Color with the Zen 2.0 Software (Zeiss).
• Autofluorescence imaging of 3D tumor-macrophage microscale cultures resolves spatial and temporal dynamics of macrophage metabolism

  Tiffany M. Heaster, et al., bioRxiv - Bioengineering 2020

  Quote: ... and 50 µL cell type-specific medium (RPMI1640 – mouse; complete DMEM/F12 (Gibco) – human) ...
• Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities

  Thomas Kruse, et al., bioRxiv - Biochemistry 2021

  Quote: ... goat anti-mouse HRP conjugated antibody (WB 1:5000, Thermo Fisher Scientific, 31430), goat anti-mouse Alexa Fluor 546 (IF 1:1000 ...
• CREB-mediated transcriptional activation of NRMT1 drives muscle differentiation

  John G. Tooley, et al., bioRxiv - Biochemistry 2021

  Quote: ... C2C12 mouse muscle cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher) with 10% FBS and 1% P/S ...
• Hoogsteen base pairs increase the susceptibility of double-stranded DNA to cytotoxic damage

  Yu Xu, et al., bioRxiv - Biochemistry 2020

  Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibody (anti-mouse IgG) was purchased from ThermoFisher. The AlkB enzyme was a gift from Dr ...
• Peptide recognition and dephosphorylation by the vaccinia VH1 phosphatase

  Bryan M. Zhao, et al., bioRxiv - Biochemistry 2020

  Quote: ... Alexa Fluor 647 conjugated goat anti-mouse antibody was purchased from Life Technologies, Inc ...
• The Mammalian Cytosolic Type 2 (R)-β-hydroxybutyrate Dehydrogenase (BDH2) is 4-oxo-L-proline Reductase (EC 1.1.1.104)

  Sebastian P. Kwiatkowski, et al., bioRxiv - Biochemistry 2021

  Quote: ... employing a mouse primary monoclonal antibody against His6-tag (MA1-4806, Invitrogen, USA) and a horseradish peroxidase-conjugated goat anti-mouse antibody (A2554 ...
• Single-cell transcriptomic resolution of stem cells and their developmental trajectories in the hippocampus reveals epigenetic control of cell state perseverance

  Adrián Salas-Bastos, et al., bioRxiv - Neuroscience 2021

  Quote: Embryonic mouse forebrain sections fixed on Superfrost Plus Microscope Slides (Thermo Fisher, USA) were surrounded with a hydrophobic pen and permeabilized and blocked with 10% normal donkey serum (NDS ...
• The E3 ligase Thin controls homeostatic plasticity through neurotransmitter release repression

  Martin Baccino-Calace, et al., bioRxiv - Neuroscience 2021

  Quote: ... For confocal microscopy Alexa-Fluor anti-mouse 488 (Thermo Fisher Scientific; 1:500) and Alexa Fluor anti-guinea pig 555 (Thermo Fisher Scientific ...
• Insula to mPFC Reciprocal Connectivity Differentially Underlies Novel Taste Neophobic Response and Learning

  Haneen Kayyal, et al., bioRxiv - Neuroscience 2021

  Quote: ... and Goat anti-mouse Alexa Fluor ® 488 (1:500 A11001 ThermoFisher®) secondary antibodies ...
• The non-adrenergic imidazoline-1 receptor protein Nischarin is a key regulator of astrocyte glutamate uptake

  Swati Gupta, et al., bioRxiv - Neuroscience 2020

  Quote: ... followed by Alexa Fluor-555-conjugated anti-mouse secondary antibody (1:1000; Invitrogen). After extensive washing ...
• Agrin/Lrp4 signal constrains MuSK activity during neuromuscular synapse development in appendicular muscle

  Lauren J Walker, et al., bioRxiv - Neuroscience 2021

  Quote: ... Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000, Invitrogen, A-21125) and/or alpha-bungarotoxin Alexa Fluor 594 (1:500 ...
• SREBP2 delivery to striatal astrocytes normalizes transcription of cholesterol biosynthesis genes and ameliorates pathological features in Huntington’s Disease

  Giulia Birolini, et al., bioRxiv - Neuroscience 2020

  Quote: ... or anti-mouse Alexa Fluor 488-conjugated goat secondary antibodies (1:500; Invitrogen) were used for detection (1h at RT) ...
• Constitutively active SARM1 variants found in ALS patients induce neuropathy

  A. Joseph Bloom, et al., bioRxiv - Neuroscience 2021

  Quote: ... and mouse anti-GFP conjugated to AlexaFlour 488 (1:250, Thermo Fisher Scientific), washed ...
• Axonal CB1 receptors mediate inhibitory bouton formation via cAMP increase

  Jian Liang, et al., bioRxiv - Neuroscience 2021

  Quote: ... and the following secondary antibodies: anti-mouse Alex647 (Life Technologies, A21241, 1:500) and anti-rabbit Alex405 (Life Technologies ...
• Super-resolution microscopy informs on the molecular architecture of alpha-synuclein inclusions in model systems and in the human brain

  Patrick Weish, et al., bioRxiv - Neuroscience 2021

  Quote: ... Alexa Fluor 555 donkey anti-mouse IgG (Life Technologies-Invitrogen, Carlsbad, CA, USA), Alexa Fluor 546 goat anti-guinea Pig IgG ((Life Technologies-Invitrogen ...
• Super-resolution microscopy informs on the molecular architecture of alpha-synuclein inclusions in model systems and in the human brain

  Patrick Weish, et al., bioRxiv - Neuroscience 2021

  Quote: ... Alexa Fluor 555 donkey anti-mouse IgG (Life Technologies-Invitrogen, Carlsbad, CA, USA), Alexa Fluor 546 goat anti-guinea Pig IgG ((Life Technologies-Invitrogen ...
• Super-resolution microscopy informs on the molecular architecture of alpha-synuclein inclusions in model systems and in the human brain

  Patrick Weish, et al., bioRxiv - Neuroscience 2021

  Quote: ... Alexa Fluor 488 goat anti-mouse IgG (Life Technologies- Invitrogen, Carlsbad, CA, USA), Alexa Fluor 555 donkey anti-mouse IgG (Life Technologies-Invitrogen ...
• Super-resolution microscopy informs on the molecular architecture of alpha-synuclein inclusions in model systems and in the human brain

  Patrick Weish, et al., bioRxiv - Neuroscience 2021

  Quote: ... Alexa Fluor 488 goat anti-mouse IgG (Life Technologies- Invitrogen, Carlsbad, CA, USA), Alexa Fluor 555 donkey anti-mouse IgG (Life Technologies-Invitrogen ...
• Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants

  Yi-Nan Zhang, et al., bioRxiv - Immunology 2021

  Quote: ... Mouse mAbs were expressed by the transient transfection of ExpiCHO cells (Thermo Fisher) with equal amounts of paired heavy and κ-light chain plasmids ...
• Tumor expressed CD95 causes suppression of anti-tumor activity of NK cells in a model of triple negative breast cancer

  Abdul S. Qadir, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Gene expression in mouse cells was quantified using specific primers from Life technologies for mGAPDH (Mm99999915_g1) ...
• MiR-212-3p functions as a tumor suppressor gene in group 3 medulloblastoma via targeting Nuclear Factor I/B (NFIB)

  Naveenkumar Perumal, et al., bioRxiv - Cancer Biology 2021

  Quote: ... membranes were washed and incubated with anti-mouse/rabbit IgG secondary antibody (Invitrogen) conjugated with horseradish peroxidase (HRP ...
• An in vivo inflammatory loop potentiates KRAS blockade

  Kristina A.M. Arendt, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and hybridized to GeneChip Mouse Gene 2.0 ST arrays (Affymetrix; Santa Clara, CA). Analyses using Affymetrix Expression/Transcriptome Analysis Consoles (Ritchie et al ...
• The Transcription Factor Xrp1 is Required for PERK- Mediated Antioxidant Gene Induction in Drosophila

  Brian Brown, et al., bioRxiv - Cell Biology 2021

  Quote: ... Alexa Fluor 647 goat anti-mouse (1:500, Life Technologies, cat. #A- 21235), Alexa Fluor 488 goat anti-chicken (1:500 ...
• Androgen signaling connects short isoform production to breakpoint formation at Ewing sarcoma breakpoint region 1 via an R-loop-dependent mechanism

  Taylor R. Nicholas, Peter C. Hollenhorst, bioRxiv - Cancer Biology 2020

  Quote: ... immunoprecipitated with 8 μl of S9.6 antibody and 100ul anti-mouse Dynabeads (Invitrogen), and DNA cleaned up using AMPure beads (Beckman Coulter Inc.) ...
• TAT-Cx43_266-283 impairs metabolic plasticity in glioma stem cells in vitro and in vivo

  Sara G. Pelaz, et al., bioRxiv - Cancer Biology 2019

  Quote: ... or mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679). After repeated washes ...
• Load adaptation of endocytic actin networks

  Charlotte Kaplan, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-clathrin heavy chain (AB X-22, MA1-065, Thermo Fisher Scientific) and rabbit anti-N-WASP (ab126626 ...
• Common variation in EDN1 regulatory regions highlights the role of PPARγ as a key regulator of Endothelin in vitro

  Mauro Lago-Docampo, et al., bioRxiv - Cell Biology 2021

  Quote: ... and Alexa Fluor 594-conjugated goat anti-mouse (ThermoFisher, #A-11005, 1:1000).
• Large-scale Phenotypic Drug Screen Identifies Neuroprotectants in Zebrafish and Mouse Models of Retinitis Pigmentosa

  Liyun Zhang, et al., bioRxiv - Neuroscience 2020

  Quote: ... The secondary antibody used was goat anti-mouse Alexa 647 (1:1000; Invitrogen).
• Proteomic and functional mapping of cardiac Na_V1.5 channel phosphorylation reveals multisite regulation of surface expression and gating

  Maxime Lorenzini, et al., bioRxiv - Physiology 2020

  Quote: ... the anti-transferrin receptor mouse monoclonal antibody (TransR, 1:1000, Thermo Fisher Scientific), and the anti-glyceraldehyde 3-phosphate dehydrogenase mouse monoclonal antibody (GAPDH ...
• Retinoic acid exerts sexually dimorphic effects over muscle energy metabolism and function

  Yaxin Zhao, et al., bioRxiv - Biochemistry 2021

  Quote: ... goat-anti-mouse IgM Alexa 555 (1:200, Thermo Fisher A-21426, AB_2535847), IRDye® 800CW goat anti-mouse IgG Secondary Antibody (1:10000 ...
• Retinoic acid exerts sexually dimorphic effects over muscle energy metabolism and function

  Yaxin Zhao, et al., bioRxiv - Biochemistry 2021

  Quote: ... goat-anti-mouse IgG1 Alexa 488 (1:200, Thermo Fisher A-21121, AB_2535764), goat-anti-mouse IgM Alexa 555 (1:200 ...
• HspB8 prevents aberrant phase transitions of FUS by chaperoning its folded RNA binding domain

  Edgar E. Boczek, et al., bioRxiv - Biochemistry 2021

  Quote: ... Secondary antibodies used were anti-mouse Alexa Fluor 594 (A-21203, Thermo Scientific) and anti-rabbit Alexa Fluor 488 (A-21206 ...
• SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors

  Chao Gao, et al., bioRxiv - Biochemistry 2020

  Quote: ... and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001, 1:500).
• Mechanical activation drives tenogenic differentiation of human mesenchymal stem cells in aligned dense collagen hydrogels

  Hyeree Park, et al., bioRxiv - Bioengineering 2021

  Quote: ... and/or AlexaFluor 555 Goat anti-Mouse IgG (1:250, A21422, Invitrogen, Canada). Where F-actin was co-stained ...
• Transient DUX4 expression induces blastomere-like expression program that is marked by SLC34A2

  Masahito Yoshihara, et al., bioRxiv - Cell Biology 2021

  Quote: ... Secondary antibody Alexa 594-conjugated donkey anti-mouse (A-21203, Thermo Fisher Scientific), was diluted 1:1000 in FACS buffer and incubated with cells on ice for 30 min ...
• Identifying Dihydropyrimidine Dehydrogenase as a Novel Regulator of Hepatic Steatosis

  Kelly E. Sullivan, et al., bioRxiv - Cell Biology 2021

  Quote: ... incubated with donkey anti-mouse IgG cross-adsorbed DyLight 800 (ThermoFisher, SA5-10172), and goat anti-rabbit IgG cross-adsorbed DyLight 680 secondary antibodies (ThermoFisher ...
• A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegans

  Chenshu Liu, et al., bioRxiv - Cell Biology 2021

  Quote: ... The following antibodies were used: anti-V5 (mouse, P/N 46-0705, Invitrogen), anti-HA (mouse monoclonal ...