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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Protein content was determined using pierce 660 nm protein assay reagent (Thermo Fisher Scientific), and BSA as the standard.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). Porablot NCP nitrocellulose membranes (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific #23227) and mixed 1:1 with 2× SDS-PAGE loading buffer (100 mM Tris at pH 6.8 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined by Pierce BSA Protein Assay Kit (Thermo Fisher, Cat #23225). Protein samples were prepared for western blot analysis by adding 4x sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were measured using the Pierce™ BCA Protein Assay Kit (Thermofisher scientific). 30 µg of total protein extracts were mixed with 1/5 volume of 5x Lämmli buffer (250 mM Tris-HCL pH 6.8 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific, 23225). Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Mitochondrial protein concentration was determined by Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using a PierceTM BCA Protein Assay Kit (Thermo Fisher ScientificTM) according to manufacturer’s instructions and adjusted to 1.025 mg/mL with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using Pierce™ BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein was added to a protein amount of 1.2 mg ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were quantified using a standard Pierce BCA protein assay kit (Thermo Scientific). Samples were normalized to 1.5µg/µl with Novex Tris-glycine SDS sample buffer with 10% BME ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Pierce BCA Protein assay kit (#23225, Thermo Fisher Scientific) and loading equalized accordingly ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein concentration was measured with Pierce BCA Protein Assay Kit (Thermo Scientific 23227) and 15 μg of total protein lysates was analyzed by western blot ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was determined by Pierce™ BCA protein assay kit (Thermo Fisher Scientific) and purity was estimated by SDS-PAGE.
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was estimated using Pierce™ BCA Protein Assay Kit (Thermofisher Scientific, USA). Equal amounts of protein were run on 4-20% gradient precast gels and transferred to PVDF membrane ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein concentrations were determined using the Qubit Protein Assay Kit (Invitrogen, catalog no. Q33212). The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and 100 µl were saved for input control ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration was quantified with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Ten µl was withdrawn for protein quantification using the Pierce BCA protein assay (ThermoFisher) and 300 ml ethanol and 1 ml of hexane were added to the remainder of the lysate ...
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bioRxiv - Genetics 2024Quote: ... The protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) and samples were diluted accordingly ...
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bioRxiv - Bioengineering 2024Quote: ... Western protein amounts were normalized to 15μg through BCA Protein Assay (Thermo Scientific, #23225). Lysates (20μg ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Bradford Protein Assay Kit (Thermo Fisher Scientific, 23200) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration of AEVs was measured by Qubit® Protein Assay (Thermo Fisher Scientific), as described above.
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bioRxiv - Microbiology 2024Quote: ... protein concentration was quantified by the BCA assay (PierceTM BCA Protein Assay kit, Thermofisher). Specific activity was calculated as the concentration of hydrolyzed nitrocefin produced per minute per mg of total protein extract ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein levels were quantified using the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Cell Biology 2020Quote: ... fusion protein containing the RhoA binding domain of rhotekin (rhotekin-GST) was performed by the manufacturer’s protocol using GTPase Pull-Down kit (Thermo Scientific, Waltham, MA, USA). Activated and total RhoA were detected by Western blot analysis using anti-RhoA antibody[15].
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bioRxiv - Developmental Biology 2021Quote: ... The gRNA oligo was annealed to the scaffold gRNA primer containing a T7 RNA polymerase binding site and the dsDNA template amplified by Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific F530S). The resulting dsDNA template was purified with a DNA clean and concentrate kit (Zymo Research ...
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bioRxiv - Developmental Biology 2021Quote: ... with mutated Zld and Su(H) binding sites in the 5kb dpn cis-regulatory and used Gateway LR Clonase (ThermoFisher Scientific, Waltham, MA) to clone the region into the VanGlow vector without the DSCP (Addgene#83342 ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin C (primary: mAb 14D12, Abcam catalog no. ab106046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α- IgM (mAb II/41 ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-LAMP reactions were performed in 25 μl volumes supplemented with 1 μM SYTO®-9 double-stranded DNA binding dye (Thermo Fisher S34854) in 96-well plates and incubated at 65 or 60 °C on a realtime qPCR machine (BioRad CFX96) ...
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bioRxiv - Pathology 2021Quote: ... in which the lac-operator and the ribosome binding site (RBS) were replaced by the 5’-UTR of the in vitro expression vector pEXP-5-CT/TOPO (Invitrogen, Karlsbad, CA, USA). For cloning ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin A (primary: mAb 8G6, Hycult catalog no. HM1035; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-mannose binding lectin C (primary ...
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bioRxiv - Biochemistry 2020Quote: ... RT-LAMP reactions were performed using WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (M1800) supplemented with 1 μM SYTO®-9 double-stranded DNA binding dye (Thermo Fisher S34854) and incubated on a real-time qPCR machine (BioRad CFX96 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2021Quote: ... Binding studies in HEK 293T cells were performed using rVSV-SARS-CoV-2 Spike virions (rVSV/Spike) and binding was performed at room temperature in IMMULON 2HB flat bottom plates (Thermo Scientific, Waltham, MA).
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Plant Biology 2022Quote: ... It was then transferred to the pLAW10 vector (with the GAL4 DNA-binding domain) with the LR cloning Gateway strategy (Thermo Fisher, http://www.thermofisher) and used as bait ...
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bioRxiv - Physiology 2024Quote: ... Cells were pelleted and washed in Annexin V binding buffer three times and then resuspended in Annexin V AlexaFluor 568 (Thermo Fisher Scientific A13202) (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Groups of heads were then homogenized in Lysis Binding Mix (from MagMAX mirVana Total RNA Isolation Kit; Thermo Fisher Scientific, Washington, DC; #A27828) using Navy Eppendorf RNA Lysis Kits (Next Advance ...
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bioRxiv - Microbiology 2024Quote: ... vectors with the mutated binding sites for LiaR and SapR were synthesized and sub-cloned into pTCVLac by GeneArt (ThermoFisher Scientific Inc., Darmstadt). Constructs for unmarked deletions in E ...
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bioRxiv - Neuroscience 2023Quote: ... and the presence of FRα-binding peptide in the nuclear fraction was shown by western blot analysis using the antibody against FRα1-binding peptide (Thermo Fisher, ref: PA5-101588).
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bioRxiv - Molecular Biology 2023Quote: Ligand-induced recruitment of SRC-1 coactivator peptide to the ligand binding domain of FXR was assayed by time-resolved FRET using LanthaScreen technology according to the manufacturer’s instructions (Thermo Fisher Scientific, cat# A15140). Concentration of test compounds ranged from 10 nM to 100 μM in 1.0% DMSO ...
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bioRxiv - Plant Biology 2023Quote: ... end-labeled oligonucleotides corresponding to class-II TCP consensus binding site were prepared with [γ-32P]-ATP (BRIT, India) using T4 polynucleotide kinase (EK0031, Thermo Scientific, USA). 15μL of binding reaction with 0.05 μM labeled oligonucleotide probe ...
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bioRxiv - Immunology 2023Quote: ... and 0.05% NaN3 in PBS with the addition of Fc blocking antibody to prevent non-specific binding for 2 hours at room temperature then washed three times in Wash Buffer (0.1% of Tween-20 (Thermo Fisher Scientific Cat# 28321) in PBS) ...