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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Following washes in RPE buffer RNA was eluted in RNase free water and analysed by electrophoresis on an agarose gel and quantified using the Qubit RNA High Sensitivity Assay Kit (ThermoFisher Q32852).
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bioRxiv - Bioengineering 2024Quote: ... 50 μL of supernatant was harvested and assayed for secreted LDH using the CyQuant LDH Cytotoxicity Assay kit according to the manufacturer’s instructions (ThermoFisher, Waltham, MA). The remaining supernatant was collected and frozen at −80° C for subsequent ELISA.
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bioRxiv - Biochemistry 2023Quote: ... The protein in the final sample was quantified using the Qubit Protein Assay Kit with the Qubit 2.0 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Samples were filtered through nitrocellulose membranes to remove the beads and protein was quantified using a Qubit 2.0 fluorometer and a Qubit™ protein assay kit (Life Technologies). 50 μg of protein sample was suspended in 50 μl of 8 M urea buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary analysis of concentration and quality was conducted by the Genomics CoRE Facility at the Icahn School of Medicine at Mount Sinai using Qubit RNA BR Assay Kit (ThermoFisher #Q10211). Library construction and RNA sequencing were performed by Novogene (Durham ...
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bioRxiv - Molecular Biology 2023Quote: ... The quality control was performed by measuring the concentration of DNA samples on a Qubit fluorometer using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher). The reduced-representation bisulfite sequencing (RRBS-seq ...
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bioRxiv - Neuroscience 2022Quote: ... The Alexa-647 labelling for detection used in SMC and in ELISA assays was performed using the Alexa Fluor-647 Monoclonal Antibody Labeling Kit from Thermo Fisher Scientific (catalog #A20186 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein in the supernatants was quantified using Pierce™ Bovine Serum Albumin (BCA) Protein Assay Kit (Thermo Fisher Scientific, Cat. 23225).
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bioRxiv - Plant Biology 2023Quote: ... RNA was eluted with 20 μl of RNase-free water and yield was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... Fragmentation was verified using agarose gel electrophoresis and RNA quantity was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
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bioRxiv - Cell Biology 2022Quote: ... The concentration of cDNA products was quantified using a Qubit Fluorometer and the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). The quality of the cDNA product was checked using an Agilent High Sensitivity DNA Kit and Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Pathology 2023Quote: ... the purified RNA concentration of the brain and spinal cord of the alligator snapping turtle and loggerhead turtle was quantified by using Qubit RNA HS assay kit (Qubit 3.0 fluorometer; Thermo Fisher Scientific) and reverse transcribed by using the SuperScript IV reverse transcriptase kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were transferred to new pre-chilled 1.5 mL tubes and protein concentrations were quantified using the Thermo Fisher Pierce BCA Assay Kit (Thermo Fisher, 23225). Protein lysates were resolved via SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate protein content was normalized using a BCA protein assay kit (Prometheus) and samples were denatured with 1x NuPAGE LDS Sample Buffer (Life Technologies) containing 5%β-Mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... We then quantified the extracted genomic DNA with a Nanodrop and Qubit™ (dsDNA BR Assay Kit [Thermo Fisher; Waltham, MA]) for DNA quality and concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of extracted DNA was measured using the Qubit instrument and the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). After extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA concentration of each extract was measured on a Qubit 3.0 fluorometer using the Qubit dsDNA BR assay kit (ThermoFisher Scientific, #Q32853). The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... and the DNA concentration was determined using a Qubit 3.0 fluorometer with the Qubit dsDNA BR assay kit (ThermoFisher Scientific, #Q32853).
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bioRxiv - Microbiology 2022Quote: ... We then quantified the extracted genomic DNA with a Nanodrop and Qubit™ (dsDNA BR Assay Kit [Thermo Fisher; Waltham, MA]) for DNA quality and concentration ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were measured using a Qubit 2.0 Fluorometer with a Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... All cells were resuspended in FACS buffer and stored on ice until flow cytometry staining of EdU+ cells using the Click-iT Plus EdU Flow cytometry assay kit according to the manufacturer’s instructions (Thermo Fisher Scienticific).
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hpi (to elucidate the effect of SP-A on viral infectivity) was determined using the BCA protein assay kit (Thermo Scientific). Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 14,000 x g at 4 °C and RNA concentration was measured using Qubit BR RNA Assay Kits (ThermoFisher Scientific, Q10210). For IP ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Protein levels of the brain tissue were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Developmental Biology 2023Quote: ... Concentration and length distribution of the RNA were checked with the Qubit RNA HS Assay Kit on a Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and the RNA 6000 Nano Kit on a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Physiology 2023Quote: ... low glucose DMEM with heat-killed FITC-labeled Escherichia coli at 250 µg/ml (strain K-12; reagents from Vybrant phagocytosis assay kit; ThermoFisher Scientific; Cat # E2861) (ThermoFisher Scientific) ± T2R agonist for 15 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... following the manufacturer’s instructions.18 DNA extracts were quantified on a Qubit4 fluorimeter using the dsDNA HS Assay Kit (Life Technologies; USA) and were stored at −20°C before further analysis ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Loss of plasma membrane integrity and cytotoxicity during treatments were assessed by measuring the lactate dehydrogenase (LDH) release in the culture medium using Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... lysed in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). Samples were denatured in Laemmli buffer at 95 °C and run at 170 V on a 10% acrylamide gel with a 4% stacking gel ...
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bioRxiv - Bioengineering 2023Quote: ... The concentration of the library was analyzed using a Qubit 3.0 Fluorometer and a dsDNA HS Assay Kit (Thermo Fisher Scientific). Library quality was tested using a Fragment Analyzer and the dsDNA 915 Reagent Kit (Advanced Analytical Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of DNA was measured using the Qubit® DNA Assay Kit in the Qubit® 3.0 Flurometer (Invitrogen, USA). For total RNA extraction ...
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bioRxiv - Biophysics 2023Quote: ... The amount of Pi released from actin was measured by absorbance at 360 nm every 20 seconds for 30 minutes to 1 hour using an EnzChek phosphate assay kit (Thermo Fisher) (63 ...
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bioRxiv - Cell Biology 2023Quote: ... lipopolysaccharide (LPS) was quantified in the media by a limulus amebocyte lysate assay (Pierce Chromogenic Endotoxin Quant Kit, Thermofisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA samples were quantified with Qubit™ dsDNA HS Assay Kit using a Qubit 4 fluorometer (Invitrogen, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Total protein within conditioned media was determined on the day of zymography experimentation and determined by using a bicinchoninic acid protein assay kit (ThermoFisher Scientific) according to the manufacturer’s protocol and measured on a Safire II (Tecan ...
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bioRxiv - Molecular Biology 2023Quote: ... An aliquot (25□l) of this lysate was used for protein quantitation using Pierce Coomassie protein assay kit (cat#23200, ThermoFisher scientific). The remaining lysate was centrifuged at 5000xg at 4C to pellet debris ...
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bioRxiv - Genomics 2023Quote: ... The DNA concentration was assessed using the Qubit Fluorometer 2.0 and the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, USA), and the DNA quality was checked by the Genomic DNA ScreenTape device (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and quantified using the fluorescent dye-based Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA). Samples were equimolarly pooled and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were quantified using a Qubit 3.0 Fluorometer and associated Qubit 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). Library quality assessment using an Agilent TapeStation system and paired-end sequencing of the Hi-C libraries were performed at the Quadram Institute Bioscience ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The quantitative real-time PCR (qPCR) assays were carried out on a StepOnePlus system (Applied Biosystem) using SYBR Green Master Kit (A25918, Thermo Fisher). Primers for qPCR were listed in Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the Cetuximab antibody was calculated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). The loading ratios and conjugation efficiencies of the two dyes were subsequently calculated using the obtained concentrations of the dyes and protein (antibody) ...