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Citations for Thermo Fisher :
7801 - 7821 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: The Kaplan–Meier plotter was used to cross-validate the effect of differentially expressed genes in an independent ovarian cancer cohort (Geo accession identifier: GSE9891).31 This replication cohort included gene expression profiling (Affymetrix Human Genome U133 Plus 2.0 Array) of 285 ovarian tumor samples ...
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bioRxiv - Molecular Biology 2019Quote: ... falciparum or human A549 total RNA was enriched by two successive rounds of purification with the Dynabeads mRNA purification kit (Thermo Fisher # 61006, Supplementary Fig. 1b,c).
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from the six EBV-induced lymphomas (tumor group) and normal human lymphocytes (normal group) using TRIZOL reagent (Ambion/Life Sciences, Grand Island, NY, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells used for transduction were obtained by magnetic separation from healthy-donor derived apheresis products (Dynabeads® Untouched™ Human CD8+ T Cells Kit, Thermo Fisher; Magnet, DynaMag™).
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bioRxiv - Immunology 2021Quote: ... as well as the human lung epithelial Calu-3 cells (ATCC® HTB-55™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNAi screen targeting 10,415 druggable genes (three individual siRNAs per gene) was conducted using OV90 cells and the Silencer® Select Human Druggable Genome siRNA Library Version 4 (Ambion Thermo Fisher Scientific, Waltham, MA), in absence or presence of bardoxolone methyl ...
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bioRxiv - Immunology 2022Quote: ... the cells were first extracellularly stained with a mouse anti-human monoclonal antibody APC-Cy7 conjugated LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (#L10119, Invitrogen, Thermo Fischer Scientific, OR, USA), following a similar preparation procedure as just described for the DCs ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Bioengineering 2024Quote: Engineered cardiac tissues and hiPSC-cardiomyocytes cultured with patient IgG were fixed and incubated with secondary anti-human IgG (Thermo Fisher Scientific, cat. no. A-11013) to visualize cellular autoantibody targets ...
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were grown to ∼80% confluence followed by transfection with 100 nM human ERG Silencer Select (ID #s4811) or nontargeting siRNA oligos (Life Technologies: ERG #4392420 and nontargeting #AM4635) using lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Physiology 2023Quote: ... and human tumor necrosis factor-α (TNF-α) used in the incubation of pancreatic β cells and isolated islets were purchased from Invitrogen (Carlsbad, CA, USA). Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary anti-human CD38 antibody was applied at a dilution of 1:250 and incubated overnight at 4°C (Thermo Scientific clone RM388 Cat# MA5-36061). After washing with PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Real time PCR analysis was performed on 1µL of cDNA using TaqMan Gene Expression Master Mix according to the manufacture’s procedures using FAM labeled TaqMan® and PrimePCR gene expression human primer/probe sets (Thermo Scientific and BioRab; see Supplementary Table 3). mRNA expression was compared to untreated control using the ΔCT method ...
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bioRxiv - Immunology 2022Quote: ... and culture supernatants were harvested after 16 h and assayed for TNF-α levels using a TNF-α human uncoated ELISA kit (Invitrogen, cat. no. 85-88-7346). The assay of CHO-K1 cells (infected with lentivirus of 3A1 and CD80 ...
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bioRxiv - Cell Biology 2024Quote: Reduction of hybrid receptor expression in HUVECs was carried out by transfection of validated human siRNA duplexes of insulin receptor (Silencer™ Pre-Designed siRNA, Invitrogen; Catalog number: AM51331, siRNA ID:29) and IGF-1 receptor (Catalog number ...
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bioRxiv - Cell Biology 2021Quote: ... and human SK-MEL-2 (hCLTAEN-Tq2 hDNM2EN-eGFP, as previously described(Scott et al., 2018)) were maintained in DMEM (Gibco, 4.5 g/L glucose, 110 mg/L pyruvate), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: Microarray transcriptomic data from UII-hFGFR3-S249C and BBN mice was combined with transcriptomic array data from human bladder tumors (CIT; Affymetrix Exon 1.0 ST; 96 MIBC and 99 NMIBC). Batch effects due to data combination were corrected using the surrogate variable analysis R package ...
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bioRxiv - Cancer Biology 2022Quote: The detection of TGF-β in the cell culture media from SH-SY5Y and MDA-MB-231 cells treated with TEPA for 24h was performed according to the protocol suggested by Thermo Fisher (TGF-beta-1-Human-ELISA-Kit/BMS249-4).