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Citations for Thermo Fisher :
7751 - 7800 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... qPCR was performed using SYBR Green Supermix (Quanta Biosciences, no. 95054) in an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). Results were analyzed in SDS 2.4 software (Applied Biosystems) ...
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bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... Taqman or SYBR Green assays were used (see Supplementary Table S5) in combination with Life Technologies Universal Master mixes and run on a 7900 HT sequence detection system (Life Technologies). Cycling conditions were 95°C ...
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bioRxiv - Microbiology 2019Quote: Metabolic EdU labeling and detection of newly synthesized DNA was performed with the Click-iT EdU flow cytometry cell proliferation assay by Thermo Fisher Scientific and Alexa Fluor 488 azide ...
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bioRxiv - Cell Biology 2019Quote: ... profilin and GAPDH from whole cell lysate were detected with Li-Cor fluorescent antibodies on an Odyssey detection system (Li-Cor) or via X-ray film after incubation with a developing reagent (Thermo Fisher), as indicated ...
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bioRxiv - Plant Biology 2019Quote: ... the slides were stained for DNA detection with Hoechst 33342 (Life Technology) and mounted in ProLong Gold antifade reagent (Life Technologies). This type of 3-step reactions allowed to locate both the total pool of RNAPol II ser2 / 5 (green fluorescence ...
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bioRxiv - Plant Biology 2019Quote: ... the slides were stained for DNA detection with Hoechst 33342 (Life Technology, USA) and mounted in ProLong Gold antifade reagent (Life Technologies).
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bioRxiv - Microbiology 2019Quote: ... The blots were developed using horseradish peroxidase-conjugated secondary antibodies in conjunction with the Super Signal West Dura chemiluminescence detection system (Thermo Fisher).
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bioRxiv - Molecular Biology 2021Quote: ... cells were labeled with 5-ethynyl-2’-deoxyuridine followed by detection by click chemistry and then flow cytometry based on the instructions of the manufacturer (ThermoFisher, C01425).
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bioRxiv - Molecular Biology 2021Quote: ... real-time PCR of RNA templates using hybridization probe detection chemistries (e.g., TaqMan® 5’-hydrolysis probes) on ABI7500 (Applied Biosystems) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody (IgG detector solution) and the signal detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The cDNA was diluted 16-fold and 5 μl was subjected to real-time quantitative PCR using gene-specific primers (listed in Supplemental Table S9) and SYBR Green PCR master mix in an ABI-7000 sequence detection system (Applied Biosystems). The samples were measured by three repeats.
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bioRxiv - Genomics 2019Quote: ... and hybridized to a custom probe set for expression analysis on the nCounter Digital Analyzer (NanoString Technologies) or cDNA-converted for detection on the 7900HT Fast Real-time PCR System (Applied Biosystems).
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bioRxiv - Genomics 2019Quote: About 30 μg of total RNA isolated with Trizol was loaded into each lane of agarose gel and blotted onto membrane with Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: ... Expression of the various genes was determined by the comparative CT method (ABI Prism 7700 Sequence Detection System User Bulletin #2, Applied Biosystems). Primer sequences of PGC-p/_ were as follows ...
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bioRxiv - Physiology 2020Quote: ... Real-time PCR was performed on an ABI 7000 sequence detection system using POWER SYBR-Green PCR Master MIX (Applied Biosystems). Primers were purchased from IDT Technologies and sequences are shown in Table 1.
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bioRxiv - Systems Biology 2020Quote: ... Sections were washed with PBS and signal was detected using the Ultravision ONE Detection System HRP polimer & DAB Plus Chromogen (Thermo Scientific) with Runx-2 positive cells were counted in five randomly selected fields from six bone sections of each group and quantified with Image-Pro plus 6.1 software.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Temperature control and fluorescence detection were performed using a Step One Plus Real-Time PCR System (Applied Biosystems; Grand Island, NY) with indicator dye (GloMelt ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR for quantitative detection of aberrantly spliced CFTR transcripts containing the 84bp cryptic exon using TaqMan master Mix (Applied Biosystems). The expression level was normalized to the transcript levels of HPRT (for FRT cells ...
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bioRxiv - Immunology 2020Quote: ... washed 5 times in TBS-T at room temperature and signals were detected using West Pico Plus ECL detection reagent (Thermo Scientific). Chemiluminescence was subsequently imaged on a BioRad gel imager.
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bioRxiv - Molecular Biology 2020Quote: ... Detection of target proteins was performed using SuperSignal West Femto Luminol/Enhancer solution and SuperSignal West Femto Peroxide Buffer (Thermo Scientific). Information about primary and secondary antibodies used are listed in Table S1.
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bioRxiv - Developmental Biology 2020Quote: ... 1:10 cDNA dilution was used for each reaction of quantitative RT-PCR analysis performed on an ABI Prism HT 7900 real-time PCR detection system (Applied Biosystems) equipped with SDS software version 2.4 (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The sections were washed once for 5 min in PBS and Lab Vision™ MultiVision Polymer Detection System (Thermo Fisher Scientific) was used according to manufacturer’s protocol with the following specifications ...
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bioRxiv - Developmental Biology 2020Quote: ... secondary antibody staining was performed with Alexa Fluor 568- or 488-labeled detection reagents (goat anti-rabbit, goat anti-mouse antibodies; Molecular Probes) at 1:200 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of qRT-PCR were carried out on Applied Biosystems StepOne Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA normalized to 18S RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Differences in the DNA content from every immunoprecipitation assay were determined by real-time PCR using the ABI 7700 sequence detection system and SYBR Green master mix protocol (Applied Biosystems). Primers used in this study are listed in table S1 ...
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bioRxiv - Cancer Biology 2020Quote: Digital PCR was used to detect and quantify IDH1 R132H in genomic DNA using QuantStudio 3D Digital PCR System and IDH1 Digital PCR Mutation Detection Assays from Thermo Fisher (Assay ID # Hs000000036_rm for c.395G>A (p ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR was performed using the QuantStudio 3 Detection System (QuantStudio Design and Analysis Software, Applied Biosystems, Foster City, CA, USA) and relative expression was calculated using the ΔCT method.
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then washed three times with TBST (1×15 min and 2x 5min washes) and were subjected to chemiluminescent detection using luminol as HRP substrate (SuperSignal™ West Pico PLUS Chemiluminescent Substrate, ThermoFisher).
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bioRxiv - Cancer Biology 2022Quote: Chemiluminescence detection was done in the FUSION FX imaging system (Vilber Lourmat) using the SuperSignal West Pico Luminol/Enhancer solution (Thermo Scientific). The Fusion Capt Advance software (Vilber Lourmat ...
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bioRxiv - Plant Biology 2022Quote: A Thermo Vanquish UHPLC system was used for chromatographic separation and a Orbitrap ID-X MS equipped with electrospray ionization (ESI) source was used for detection of metabolites (ThermoFisher Scientific). For the determination of amino acids ...
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bioRxiv - Microbiology 2022Quote: ... cell proliferation was quantified by reading out fluorescence with excitation at 485nM and emission detection at 530nM in the VarioSkan Lux Microplate reader (Thermo Fisher). All conditions were performed in quadruples and normalized to mock treated cells.
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR detection of the ATI gene of MPXV was performed using TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Protein bands were visualized using ECL Prime Western Blotting Detection Reagent (Cytiva; RPN2236) as per the manufacturer’s instructions and the iBright™ Imaging System (ThermoFisher Scientific). Image analysis and band quantification was performed using the iBright™ Analysis Software (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: The detection and quantitation of reactive oxygen species (ROS) was performed by monitoring luminescence using a microplate luminometer (Luminoskan, Thermo Fisher). Leaf discs (0.5 cm diameter ...
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bioRxiv - Molecular Biology 2022Quote: ... SYBR Green real-time PCR was performed using the ChamQ Universal SYBR qPCR master mix (Vazyme Biotech Co., Ltd.) and ABI 7500 FAST sequence detection system (Life Technologies). Primer sequences are listed in Extended Data Table 1.
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bioRxiv - Neuroscience 2022Quote: To analyze effector caspase activity in stimulated hippocampal neurons we employed the CellEvent(tm) caspase-3/7 green detection assay (ThermoFisher Scientific) that allows analyzing activity in individual cells ...
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bioRxiv - Microbiology 2022Quote: ... Protein bands were measured using the UVP ChemStudio imaging system (Analytik Jena) after staining each membrane with chemiluminescence detection reagents (Thermo Fisher). Housekeeping protein β-actin (a cytoskeleton protein ...
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bioRxiv - Plant Biology 2024Quote: ... Signal was detected using the ECL Prime Western Blotting Detection Reagent (Amersham) or SuperSignalTM West Femto Maximum Sensitivity Substrate (Thermo Fisher).
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bioRxiv - Immunology 2024Quote: ... and the cells were fixed for 10 minutes in 2% Formaldehyde at room temperature before detection on the Attune NxT Flow Cytometer (ThermoFisher Scientific). FCS files were analyzed using FlowJo v10.
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bioRxiv - Pathology 2024Quote: ... Immobilized antibodies were detected by UltraVision-LP HRP Polymer detection system specific for anti-mouse and anti-rabbit IgG (ThermoFisher Scientific), or with biotinylated secondary antibodies and AB reagents (Vector Laboratories ...
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bioRxiv - Physiology 2024Quote: ... was performed using a TaqMan 7900 sequence detection system with TaqMan universal PCR master mix and TaqMan gene expression assays (Applied Biosystems). Relative gene expression for Vdr ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplification and detection of 5′ tRNA-fMet halves were performed using the TaqMan Universal PCR Master Mix (#4304437, ThermoFisher Scientific) as well as custom primers and probe design to target both 5′ tRNA-fMet halves specifically.
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bioRxiv - Neuroscience 2024Quote: ... and Tris-stock (1x 5min, 50mM Tris, pH = 7.6) and incubated for 5 minutes in ECL solution for chemiluminescent detection (Thermo Fisher Scientific). Proteins were visualized using the BioRad Imaging system with Chemi visualization.
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bioRxiv - Microbiology 2024Quote: Samples were diluted 1:10 in water and were analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD, Dionex ICS-6000 HPIC System, Thermo Scientific) using a CarboPac PA100 column (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA levels were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) using the following PCR primers on a ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). Primer sequences ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Membranes were then incubated with the corresponding counter-antibody and the proteins were revealed by enhanced chemiluminescence detection (SuperSignal West Femto System, Thermo Scientific). For information about the antibodies used please see Supplementary Table S3 ...
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bioRxiv - Immunology 2024Quote: ... mRNA levels of indicated genes were quantitatively determined by SYBR-green technology on an ABI-StepOnePlus Sequence Detection System (Applied Biosystems). Sequence of primers used for RT-qPCR analysis were listed in Table S2.
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bioRxiv - Cell Biology 2023Quote: ... LC3 and GAPDH from whole cell lysate were detected with Li-Cor fluorescent antibodies on an Odyssey detection system (Li-Cor) or via X-ray film after incubation with a developing reagent (Thermo Fisher), as indicated ...