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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (Thermo Fisher Scientific), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and 6 µg of psPAX2 plasmids into 293T cells grown in a 10-cm dish using Lipofectamine 2000 (Invitrogen). The cell culture supernatant was collected 48 h after transfection and used to transduce parental HeLa cells in the presence of polybrene (8 µg/mL) ...
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... The transcribed RNA was then separated on a 6% TBE-Urea (7M urea) polyacrylamide gel (Thermo Fisher Scientific, EC6865BOX) which was post stained with GelRed (Biotium ...
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bioRxiv - Genomics 2024Quote: ... 3 µg of each plasmid was mixed with 6 µl of Plus reagent and 7.5 µl of Lipofectamine LTX (Invitrogen) reagent ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Bone marrow-derived mesenchymal stromal cells from C57BL/6 mice (Cyagen) were cultured in MEMα supplemented with GlutaMAX (Gibco) in 15% fetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse Transfection of rescue plasmid was performed in 6-well plates using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) to the proportions of 5 μL of 2 μg plasmid per well ...
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bioRxiv - Cell Biology 2024Quote: ... Around 1.6-3.2μg of digested DNA was used to synthesise mRNA with the T3 mMESSAGE mMACHINE kit (AM1348, ThermoFisher Scientific). The mRNA product was purified with the RNeasy MinElute Cleanup Kit (74204 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded in a 6-well plate at a cell density of 200,000/cm2 in Dulbecco’s Modified Eagle Media (DMEM, Thermo Fisher) supplemented with 15% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... and rested for ∼6 hours in R15 (RPMI 1640 medium supplemented with 15% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific] ...
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bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
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bioRxiv - Cell Biology 2023Quote: Cultured N2A control and Fmr1 CRISPR/Cas9 KO cells were seeded in 6-well plates overnight and then transfected using 7.5 µl of Lipofectamine 3000 (Invitrogen) and 5 µl of P3000 with the 1 µg of pFlareA-Mapt exon4 splicing reporter ...
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bioRxiv - Bioengineering 2023Quote: ... and liquid agarose (2.5mL) was used to cover the bottom of a high-performance #1.5 6-well plate (Fisher Scientific). This process was repeated for all five wells ...
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bioRxiv - Genomics 2023Quote: ... and 2µL of primers at 3µM in a total reaction volume of 10µL on qPCR was realized on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher). Each sample was tested in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with 1µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Following 6 days of expansion the filtered cell culture supernatant was applied to a 5ml Ni-NTA column (ThermoFisher) prewashed with 100ml washing solution (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-23,36-39 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-59 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells from each embryo were then plated onto a 6-cm culture dish coated with 0.1% gelatin and cultured in DMEM (Hyclone) supplemented with 10% FBS (GIBCO), 1% GlutaMAX (GIBCO) ...
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bioRxiv - Cancer Biology 2023Quote: ... normalized to the expression of GAPDH on a QuantStudio 6 and 7 Pro real-time PCR system (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Scientific’s SYBR green Real-time PCR master mix was used to perform qRT-PCR (Quantstudio 6, ThermoFisher Scientific) in triplicates to determine the fusion gene expression levels using PRCC-TFE3 fusion gene primers (FP:5’-CCAAGCCAAAGAAGAGGAAA-3’ and RP:5’- CGAGTGTGGTGGACAGGTACT-3’).
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant containing secreted pro-HlyA protein was collected by centrifugation (Thermo Scientific, Sorvall RC 6+, SLA-3000 (Rotor), 10,000 × g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined by quantitative real-time PCR using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative gene expression was determined using ΔΔCT method ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293A cells were initially plated at 750k cells/well in 6-well plates in Dulbecco’s modified eagle media (DMEM) supplemented with 10% fetal bovine serum (FBS) and GlutaMax (Gibco) with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were assembled for 20 min at room temperature and separated on 6% DNA retardation gels (Thermo Fisher scientific) at 200 V for 1–2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg FLAG-AGAL construct and 4.5 µg pOG44 was transfected into HFTs seeded onto a 6-well plate using Lipofectamine 2000 (Invitrogen). After 48 hours the transfected cells were selected with hygromycin (200 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... 8 days after transfection antibiotic selection was performed using 6 µg mL-1 blasticidin S (Thermo Fisher Scientific, A1113903) to identify cells which had undergone productive editing ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with the primers for ICP27 and β-actin on a Quant Studio 6 Flex qPCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... and an oxygen-scavenger system (60□mg ml−1 glucose oxidase, 6□mg□ml−1 β-ME (Gibco, 21985023)) were used for all STORM imaging experiments.
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and SEC using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded at a concentration of 150,000 cells per well of a Matrigel-coated 6-well plate in E8 medium (ThermoFisher) containing Rock-inhibitor (10 µM ...