Labshake search
Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for Recombinant Human WIBG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
-
bioRxiv - Cancer Biology 2024Quote: ... The protein content of the resulting lysate was quantified using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and 25 µl of the supernatant was used to determine the protein amount following the manufacturer’s instructions using a Pierce BCA Protein Assay Kit (Cat#23225, Thermo Scientific). A vial containing a quality control mixture pool of 12.5 µl from each sample (150 µl total ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 μg protein was loaded onto BoltTM 4-12% Bis-Tris mini protein gels (catalog #NW04125BOX) or NovexTM 14% Tris-Glycine mini protein gels (catalog #XP00145BOX) from Invitrogen. Electrophoresis was run at neutral pH in NuPAGETM MOPS running buffer (catalog #NP0001 ...
-
bioRxiv - Neuroscience 2024Quote: ... The concentration of the resulting protein pellet was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Cat. 23225) and a NanoDrop™ 2000/2000c spectrophotometer (ThermoFisher) ...
-
bioRxiv - Neuroscience 2024Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
-
bioRxiv - Molecular Biology 2024Quote: ... The sample protein concentration was measured using the Micro BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA; Cat.: 23235). 20 μg of total protein lysate was resolved by electrophoresis on Bis-Tris 4-12% NuPAGE polyacrylamide gels using the Novex Mini Cell system (Invitrogen/Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2024Quote: ... The protein concentration in the MFS was quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat. # 23225), using BSA as a standard ...
-
bioRxiv - Immunology 2024Quote: ... the phage:antibody complexes were immunoprecipitated using 40 μL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) (4°C for 4 hours ...
-
bioRxiv - Developmental Biology 2024Quote: ... and the cytosolic and membrane proteins were separated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher: 89842) with cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Microbiology 2024Quote: ... was added to a 255μl H1N1/pdm09 suspension [2mg/ml of viral protein quantified using the modified Lowry protein assay (Thermo Scientific™ Pierce™ Modified Lowry Protein Assay Kit ...
-
bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
-
bioRxiv - Cell Biology 2023Quote: ... The plate was gently agitated for 20 minutes at room temperature and the protein concentration was measured using a Pierce BCA Protein Assay Kit (cat. #0023227, Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: Total protein concentration in the purified samples was determined with the Pierce Rapid Gold BCA Protein Assay kit (ThermoFisher A53225) by comparing absorbance signal at 480 nm to a BSA standard curve ranging from 0-2000 μg/ml using manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... proteins were extracted in cold RIPA lysis buffer and supernatant was used to determine protein concentration using the Pierce BCA protein assay kit (Thermofisher). 50 μg protein was loaded on Mini-PROTEAN TGX Stain-Free Gels before transfer to Nitrocellulose membranes (Biorad) ...
-
bioRxiv - Developmental Biology 2022Quote: Proteins from WT C57BL/6J testis were isolated utilizing the T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with Halt Protease Inhibitor Single-Use Cocktail EDTA-Free (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 5,000 xg and protein concentration was quantified by Pierce BCA Protein Assay (Thermo Fisher).
-
bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein concentration determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Sci., Cat. no: 23225).
-
bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein concentration was quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... total proteins from RVLM-containing tissue punches were extracted using N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: The protein concentrations of renal samples were determined with a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, USA). Protein samples (50 μg ...
-
bioRxiv - Neuroscience 2023Quote: ... Total protein levels were quantified using a Pierce™ Microplate BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). A total of 30 μg protein was separated by SDS-PAGE at 200 V for 45 min using a 4%–12% precast polyacrylamide gel (Novex ...
-
bioRxiv - Biochemistry 2022Quote: ... The For3 was diluted and spun at 400,000 g for 30 min each time before use and the protein concentration in the supernatant was determined by Bradford protein assay (ThermoFisher). The protein gradually loses its activity on ice overnight.
-
bioRxiv - Cell Biology 2023Quote: ... The protein concentrations of the both the tissue and cell lysates were assessed by BCA Protein Assay Kit (ThermoFisher #23227) and protein samples were supplemented with 4x laemmli blue (Bio-Rad #1610747 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Purity of histones was evaluated by SDS-PAGE and protein concentration was determined using Qubit™ Protein Assay Kit (Invitrogen). Histones were digested using the Propionylation-PIC method (Maile et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... Soluble protein content was quantified using the Pierce™ BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Hampton, NA, USA), and the samples were diluted to a final concentration of 1 mg/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... the amount of protein per sample was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat. 23225). Trypsin digestion ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% of input chromatin was saved and 70 μg of chromatin pre-clearing was done by mixing with pre-blocked Sepharose beads (1:1 vol. mix of Protein A and protein G beads; Invitrogen) for 1h at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... each sample’s total protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA).
-
bioRxiv - Genetics 2023Quote: ... the concentration of each protein fraction was quantified in duplicate using a Pierce BCA protein assay kit (Thermo Scientific #23225) and results were read on a Synergy H1 Microplate Reader using BioTek Gen5-3.04 software per manufacturer instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were obtained using the BCA Protein Assay Kit (Pierce) and 6X Laemmli SDS sample buffer (Thermo Fisher Scientific) was added to protein extracts ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total protein concentration of the cell lysates was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No. 23227). Cell lysates were mixed with the sample buffer (with beta mercaptanol ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected into a new 1.5 ml tube and protein concentration was measured using the bovine serum albumin protein standard assay (Cat.No.: 23208, Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected in a new tube and protein concentrations were measured using a BCA protein assay kit (Thermo Fisher).
-
bioRxiv - Molecular Biology 2023Quote: ... 600 μg of chromatin was precleared with 30 μl of Protein A- or Protein G-coupled magnetic beads (Invitrogen Dynabeads) for one hour at 4°C on a rotating platform ...
-
bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
-
bioRxiv - Systems Biology 2023Quote: ... The protein concentration was determined with a BCA protein Assay kit following the instructions from the vendor (23225, Thermo Scientific).
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged for 5 minutes at 16,000 x g and supernatant protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: Bacterially expressed and purified NEMO proteins were labeled with the Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher, A30006) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... 55,087 protein count) and contaminant protein database (provided by the manufacturer) in Proteome Discoverer software version 2.3 (Thermo Scientific, USA). A maximum of two trypsin cleavages were allowed with a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.8 Da ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a mouse anti-biotin primary antibody at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... The degree of labeling of the proteins with the fluorescent probes was evaluated by following the manufacturer’s protocol (Microscale Protein Labeling Kit, ThermoFisher Scientific).
-
bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration in the lysates were determined by using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific #23225). Briefly ...
-
bioRxiv - Cancer Biology 2023Quote: To detect protein via western blotting 60µg of protein was resolved on SDS-PAGE 4- 12% Bis-Tris Bolt gels (Life Technologies). Protein was transferred onto a nitrocellulose membrane using a Mini Trans-Bolt Cell (Bio-Rad Laboratories) ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 μl were saved at −80ºC for posterior determination of protein content using Pierce BCA Protein Assay Kit (Cat. # 23227, Thermo Scientific). Samples were then immediately heat-inactivated and cooled down to room temperature.
-
bioRxiv - Developmental Biology 2023Quote: ... The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™, 23225).