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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for Recombinant Human TNFRSF21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... was added to a 255μl H1N1/pdm09 suspension [2mg/ml of viral protein quantified using the modified Lowry protein assay (Thermo Scientific™ Pierce™ Modified Lowry Protein Assay Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μg protein was loaded onto BoltTM 4-12% Bis-Tris mini protein gels (catalog #NW04125BOX) or NovexTM 14% Tris-Glycine mini protein gels (catalog #XP00145BOX) from Invitrogen. Electrophoresis was run at neutral pH in NuPAGETM MOPS running buffer (catalog #NP0001 ...
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bioRxiv - Genomics 2024Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit according to manufacturer’s guidelines (Thermo Fisher; Cat. No. 23225). Protein samples were mixed with 4X NuPAGE™ LDS Sample Buffer (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of the resulting protein pellet was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Cat. 23225) and a NanoDrop™ 2000/2000c spectrophotometer (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and the cytosolic and membrane proteins were separated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher: 89842) with cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Genetics 2024Quote: ... The protein concentration in the MFS was quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat. # 23225), using BSA as a standard ...
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bioRxiv - Immunology 2024Quote: ... the phage:antibody complexes were immunoprecipitated using 40 μL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) (4°C for 4 hours ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentration of the cleared lysate was assessed with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). IL-1β was quantified from hippocampi tissue lysates with V-PLEX Mouse IL-1β Kit (Meso Scale Discovery ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentrations in CA1 and amygdala homogenates were quantified using Pierce BCA Protein Assay (Thermo Scientific Cat. No. 23225).
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined in a 96-well format using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... The protein content of the resulting lysate was quantified using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to manufacturer instructions ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and the resulting supernatants were collected and assayed for protein concentration with a BCA Protein Assay Kit (Thermo Fisher, 23225). Samples containing equal amounts of protein were denatured by boiling for 10 min with Laemmli sample buffer and then fractionated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate was cleared by centrifugation at 11,000 g for 5 min and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein concentration was quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... total proteins from RVLM-containing tissue punches were extracted using N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: The protein concentrations of renal samples were determined with a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, USA). Protein samples (50 μg ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein levels were quantified using a Pierce™ Microplate BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). A total of 30 μg protein was separated by SDS-PAGE at 200 V for 45 min using a 4%–12% precast polyacrylamide gel (Novex ...
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bioRxiv - Biochemistry 2022Quote: ... The For3 was diluted and spun at 400,000 g for 30 min each time before use and the protein concentration in the supernatant was determined by Bradford protein assay (ThermoFisher). The protein gradually loses its activity on ice overnight.
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bioRxiv - Cell Biology 2023Quote: ... The protein concentrations of the both the tissue and cell lysates were assessed by BCA Protein Assay Kit (ThermoFisher #23227) and protein samples were supplemented with 4x laemmli blue (Bio-Rad #1610747 ...
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bioRxiv - Cancer Biology 2023Quote: ... Purity of histones was evaluated by SDS-PAGE and protein concentration was determined using Qubit™ Protein Assay Kit (Invitrogen). Histones were digested using the Propionylation-PIC method (Maile et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Soluble protein content was quantified using the Pierce™ BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Hampton, NA, USA), and the samples were diluted to a final concentration of 1 mg/mL ...
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bioRxiv - Synthetic Biology 2022Quote: Total protein concentration in the purified samples was determined with the Pierce Rapid Gold BCA Protein Assay kit (ThermoFisher A53225) by comparing absorbance signal at 480 nm to a BSA standard curve ranging from 0-2000 μg/ml using manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were extracted in cold RIPA lysis buffer and supernatant was used to determine protein concentration using the Pierce BCA protein assay kit (Thermofisher). 50 μg protein was loaded on Mini-PROTEAN TGX Stain-Free Gels before transfer to Nitrocellulose membranes (Biorad) ...
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bioRxiv - Developmental Biology 2022Quote: Proteins from WT C57BL/6J testis were isolated utilizing the T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with Halt Protease Inhibitor Single-Use Cocktail EDTA-Free (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 5,000 xg and protein concentration was quantified by Pierce BCA Protein Assay (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein concentration determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Sci., Cat. no: 23225).
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Systems Biology 2023Quote: ... The protein concentration was determined with a BCA protein Assay kit following the instructions from the vendor (23225, Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged for 5 minutes at 16,000 x g and supernatant protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Molecular Biology 2023Quote: Bacterially expressed and purified NEMO proteins were labeled with the Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher, A30006) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected into a new 1.5 ml tube and protein concentration was measured using the bovine serum albumin protein standard assay (Cat.No.: 23208, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein concentration of the cell lysates was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No. 23227). Cell lysates were mixed with the sample buffer (with beta mercaptanol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were obtained using the BCA Protein Assay Kit (Pierce) and 6X Laemmli SDS sample buffer (Thermo Fisher Scientific) was added to protein extracts ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected in a new tube and protein concentrations were measured using a BCA protein assay kit (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... 600 μg of chromatin was precleared with 30 μl of Protein A- or Protein G-coupled magnetic beads (Invitrogen Dynabeads) for one hour at 4°C on a rotating platform ...
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bioRxiv - Genetics 2023Quote: ... the concentration of each protein fraction was quantified in duplicate using a Pierce BCA protein assay kit (Thermo Scientific #23225) and results were read on a Synergy H1 Microplate Reader using BioTek Gen5-3.04 software per manufacturer instructions.
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bioRxiv - Immunology 2023Quote: ... 55,087 protein count) and contaminant protein database (provided by the manufacturer) in Proteome Discoverer software version 2.3 (Thermo Scientific, USA). A maximum of two trypsin cleavages were allowed with a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.8 Da ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration in the lysates were determined by using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific #23225). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: To detect protein via western blotting 60µg of protein was resolved on SDS-PAGE 4- 12% Bis-Tris Bolt gels (Life Technologies). Protein was transferred onto a nitrocellulose membrane using a Mini Trans-Bolt Cell (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μl were saved at −80ºC for posterior determination of protein content using Pierce BCA Protein Assay Kit (Cat. # 23227, Thermo Scientific). Samples were then immediately heat-inactivated and cooled down to room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™, 23225).
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bioRxiv - Immunology 2023Quote: ... Protein concentrations were measured for each sample using the A280 NanoDrop method and a protein standard curve (ThermoFisher Cat. 23208). Afterwards ...