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Citations for Thermo Fisher :
7551 - 7600 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Affymetrix Human Exon 1.0 ST arrays were hybridized by GenoSplice technology (www.genosplice.com) according the Ambion WT protocol (Life Technologies, France) and Affymetrix (Santa Clara ...
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bioRxiv - Microbiology 2020Quote: ... Meso Scale Discovery (MSD)) were coated with mouse anti-human IgG antibody (CH2 domain, cat# MA5-16929, ThermoFisher Scientific) at 2 μg/mL in 1X PBS (50μL/well) ...
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bioRxiv - Microbiology 2021Quote: Human foreskin fibroblasts (HFFs) were seeded in 96 wells plates and grown to confluency in DMEM medium (ThermoFisher 11960044) at 37°C in 5% CO (26) ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of total RNA was depleted of rRNAs by using the Human/Mouse RiboMinus Transcriptome Isolation Kit (Invitrogen). Ribosomal RNA depletion was confirmed by an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... The slides were washed and incubated for 2 h with secondary antibodies (Alexa 488 anti-Human IgG Thermo Fisher - #A11013 and Alexa Fluor 555 Anti-Mouse IgG #A21422) ...
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bioRxiv - Neuroscience 2021Quote: Human ESCs and iPSCs were maintained on plates coated with geltrex (life technologies, A1413301) in StemFlex media (Gibco, A3349401) and passaged with accutase (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... ATCC CCL-81) and human embryonic kidney cells (HEK293T; ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle media (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Microbiology 2020Quote: ... ATCC CRL-1586) and HEK-293T (human embryonic kidney cells) cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco). All media were supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgG labeled with FITC (Chemicon) or goat anti-rabbit IgG labeled with Alexa 488 (Thermo Scientific) was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Neuroscience 2020Quote: The quantitation of soluble human Aβ1-42 in hippocampus extracts was determined with a sandwich antibody ELISA kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Human adenocarcinomic lung epithelial (Calu-3) cells (ATCC, HTB-55) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... APOE levels were similarly processed and quantified from iMGL conditioned media using Apolipoprotein E Human ELISA kit (Invitrogen; #EHAPOE).
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bioRxiv - Neuroscience 2022Quote: ... Caco-2 and human skin fibroblast cells were grown in Dulbecco’s modified Eagles medium (DMEM) with Glutamax (Life Technologies) and supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... Human vaginal epithelium cells (hVECs, VK2/E6E7) were cultivated in Keratinocyte-Serum Free medium (Thermo Fisher Scientific, Massachusetts, USA) at 37°C in 5% CO2 as the suggestion by ATCC.
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bioRxiv - Neuroscience 2022Quote: A 448 nt fragment of human SYN1 promoter was PCR amplified with Phusion high fidelity DNA polymerase (Thermo Scientific) and the following primers ...
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bioRxiv - Immunology 2022Quote: ... The detection antibody used was horseradish peroxidase (HRP)–conjugated goat anti-human IgG (H+L) (ThermoFisher, cat# SA5-10283) at a 1:2000 dilution ...
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bioRxiv - Immunology 2022Quote: Fn purified from human plasma (sigma) or monoclonal antibodies were fluorescently conjugated using AlexaFluor 488 and 647 Ester (Invitrogen). The conjugation and purification were done according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... Human monocyte-derived macrophages (MDMs) were derived from CD14+ peripheral blood monocytes by culturing cells in RPMI/1640 (Gibco) media containing 10% heat-inactivated human AB serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Human U937 macrophages (ATCC, Cat. No. CRL-1593.2™) were maintained in RPMI (Thermo Fisher Scientific, Cat. No. 11875093) supplemented with 10% FBS (RMBIO ...
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bioRxiv - Cell Biology 2022Quote: The cDNA encoding full-length human CLASP2a was purchased from Dharmacon (Accession: BC140778.1) and subcloned into: (i) a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag and ...
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bioRxiv - Molecular Biology 2019Quote: Human MZF1 cDNA was subcloned from pOTB-MZF1 into pcDNA3-Flag vector via TOPO directional cloning (Thermo Fisher Scientific) and designated pcDNA3/Flag-MZF1 ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Immunology 2020Quote: Whole genome transcript analysis was performed on 200 ng of total RNA using the Affymetrix Human Gene ST version 2.0 Array (Affymetrix). Genome-wide DNA methylation was profiled using the Illumina EPIC platform (Illumina ...
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bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; Thermo Fisher Scientific) and the human HCC cell line HuH7 (ATCC ...
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bioRxiv - Immunology 2019Quote: A cDNA encoding wild-type (WT) human TRIM22 (a gift form Professor Juergen Hass, University of Edinburgh) was inserted into pcDNA.3.1 (Invitrogen) in frame with a 5’ Myc-tag oligo to generate a pcDNA.Myc.TRIM22 ...
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bioRxiv - Cell Biology 2020Quote: ... For RHBDL4 and OST4 depletion 50 pmol (RHBDL4) or 25 pmol (OST4) ON-TARGETplus SMARTpool human siRNA (Dharmacon) and 2.5 μl RNAimax (Invitrogen) transfection reagent per well of a 12-well plate were used ...
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bioRxiv - Cell Biology 2019Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (TERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS and 1% penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: Copy number analysis was derived from profiling of total DNA on the Affymetrix genome-wide human SNP Array 6.0 chip using instrumentation and protocols from Affymetrix. The raw CEL files were returned and the QC steps were performed using Affymetrix Genotyping Console ...
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bioRxiv - Genomics 2019Quote: Human islets were washed once in cold PBS and dissociated into single cells by enzymatic digestion with Accumax (Invitrogen), followed by digestion using freshly prepared Dispase (Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... with 12.5 pmol of either non-targeting negative control siRNA showing no homology to the human transcriptome (#4457287, Ambion), or two different siRNAs targeting OAS1 (siRNA-1 or −2 ...
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bioRxiv - Immunology 2020Quote: ... T cells were polyclonally expanded from freshly isolated PBMCs using Dynabeads human T-activator CD3/CD28 beads (Life Technologies) as previously described (Loef et al ...
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bioRxiv - Molecular Biology 2020Quote: Human HCT116 cells (ATCC) were cultured at 37°C under 5% CO2 in McCoy’s 5A media (Thermo Fisher Scientific) with 1x penicillin/streptomycin and 10% FBS (Invitrogen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 day prior to spinfection cells were activated using Human T-activator CD3/CD28 Dynabeads (Thermo Fisher, 11131D) and cultured in 200U/ml IL-2 ...
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bioRxiv - Neuroscience 2020Quote: Fresh-frozen human postmortem brain tissues were sectioned at 14-16 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes at −20°C and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Cancer Biology 2021Quote: Non-targeting siRNA and siRNA against mouse and human LIG3 were transfected into the cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Human glioblastoma cell line U-251MG was cultured in Dulbecco’s Modified Eagle Medium (DMEM-HG) (11995-065; Gibco, USA) and supplemented with 10% FBS (RM10409 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays: human GAPDH control ID: Hs99999905_m1(ThermoFisher), zebrafish abcb4 ID ...
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bioRxiv - Cancer Biology 2020Quote: ... wells were filled with 250μl of human liver organoid isolation medium consisting Ad+++ supplemented of 1X B27 supplement without retinoic acid (Gibco), 1X N2 supplement (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in complete medium in the absence of human serum or Pf-iRBCs schizonts were isolated in RPMI 1640 medium supplemented with 0.25% Albumax (GIBCO, Invitrogen) and 7.4% Sodium Bicarbonate (GIBCO ...
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bioRxiv - Bioengineering 2021Quote: ... Another subset of slides was labeled with Alexa 568 conjugated anti-human IgG antibody (1:800, ThermoFisher, A-21090) to detect the Fc portion of the EphA4-Fc chimera peptide.
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: Microarray analyses were conducted using the human Clariom D Affymetrix platform following the manufacturer’s instructions (902915, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... the coding sequence of the current human textitTRBP reference (RefSeq NM_134323) was amplified and subcloned into the pcDNA3-cMyc vector (Invitrogen) at the BamHI and XhoI sites ...
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bioRxiv - Cancer Biology 2020Quote: CAFs were isolated from human colon carcinoma resection specimens following 2.4U/ml dispase + 100U/ml collagenase type II (both from Invitrogen); disaggregation of tumour fragments and digestion for 2h t 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to the following formulation of EC medium: human endothelial serum-free medium (hESFM, Thermo Fisher 1111044) supplemented with 1% platelet-poor plasma-derived serum (PDS ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.