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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Eluates containing the enriched phosphopeptides were dried down and subsequently resuspended with 50 μl 0.1% formic acid for peptide concentration measurement using the Pierce Quantitative Colorimetric Assay kit (Thermo Scientific PN23275).
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bioRxiv - Plant Biology 2020Quote: ... Whole genomic DNAs were quantified using the Qubit dsDNA BR Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, California, USA); high-molecular-weight DNA (>15 kb ...
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bioRxiv - Bioengineering 2020Quote: ... The efficiency of siRNA encapsulation N+:P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher Scientific, Waltham, MA). siNP size was evaluated using dynamic light scattering (DLS ...
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bioRxiv - Neuroscience 2020Quote: ... 8 µg of the cDNA from each sample were fragmented and labelled with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753). Hybridization was performed as described previously31 during 16 h at 45°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The dried lipids were solubilized in 95% ethanol and the cholesterol was quantitated using the Amplex Red Cholesterol Assay kit (Invitrogen A12216). All values for the wild-type and mutant samples are shown in Table S9 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were cleared of debris by centrifugation at 14,000g for 15 min and quantified using the BCA protein assay kit (Thermo Fisher Scientific). Lysates were diluted 1:4 with 1X sample buffer (ProteinSimple) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell cycle analysis was performed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: The autophagic flux assay was carried out using the Premo™ Autophagy Tandem Sensor RFP-GFP-LC3 Kit (P36239, Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... The cartilage explants were then rinsed and incubated in PBS containing 0.05% calcein acetoxymethyl and 0.2% ethidium homodimer-1 from the Live/Dead Cell Viability Assays kit (Life technologies, Cat.# L3224) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... The surface concentration of the antibodies was quantified as previously described using a bicinchoninic acid assay (BCA) kit (Thermo Fisher 23227)36.
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bioRxiv - Bioengineering 2020Quote: ... luminescence was compared to a ladder of recombinant Gaussia Luciferase (NanoLight #321-500) quantified using a Gaussia Luciferase Glow Assay Kit (ThermoFisher #16161) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... to improve sensitivity and loading in ELISA or BLItz binding assays (EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit, ThermoFisher 21435).
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bioRxiv - Genomics 2021Quote: ... the protein concentration of each of the liquids in which the worms were incubated (“secretome” samples) was measured using a Pierce Coomassie (Bradford) Protein Assay Kit (Fisher Scientific). Bovine Serum Albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the Pierce™ BCA Protein Assay kit (Thermo Scientific). 25 μg of the lysates were then separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies). RNA libraries were sequenced as 100 bp paired-end runs on an Illumina HiSeq2500 platform ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was resuspended in water and quantified with Qubit dsDNA BR Assay kit on Qubit 2.0 Fluorometer (Thermo Fisher Scientific). DNA integrity was assessed with Genomic DNA ScreenTape on TapeStation system (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: MiR-212-3p expression was quantified using TaqMan™ MicroRNA Reverse Transcription Kit and TaqMan™ Advanced miRNA Assay (Applied Biosystems). Isolated total RNA was reverse transcribed using a specific stem-loop primer for miR-212-3p and RNA U6B ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged at 4 °C for 10 minutes at 16000 g to remove cell debris and protein concentration of the lysate was measured using Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts (10 µg ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of the lipid extract was used to estimate the total sterol content using the Amplex™ Red Cholesterol Assay Kit (Invitrogen) per the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: NIH 3T3 cell proliferation was quantified according to the CyQUANT® Cell Proliferation Assay Kit (Molecular Probes, Inc. Eugene OR, USA) at four cell densities 1600 – 6700 cells/well ...
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bioRxiv - Cell Biology 2021Quote: ... the tissue was homogenized with RIPA cell lysis buffer (Milipore) and then lysate was quantified with Pierce BCA protein assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentrations of the supernatants were determined using a Pierce BCA protein assay kit (Thermo Scientific, catalog # 23225, lot # SJ256254) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and the relative cell numbers in the individual wells were assessed using the CyQuant Cell Proliferation Assay Kit (Molecular Probes, #C7026). Seahorse records were corrected for well-to-well differences in the cell numbers and analyzed using Wave software (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A small volume of each sample was used to immediately measure protein concentration with the Pierce BCA Protein Assay kit (ThermoFisher; 23227). The rest was combined with 6X loading dye (375mM Tris pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were homogenised using a VDI 12 tissue homogeniser and protein was quantified using a bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific). 100 μg of total lung protein were lysed in 2x sample buffer (4% [w/v] SDS ...
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bioRxiv - Microbiology 2020Quote: ... Cytotoxicity was measured by quantifying lactate dehydrogenase (LDH) from ARPE-19 (Pierce LDH Cytotoxicity Assay Kit, ThermoFisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions [12,20].
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bioRxiv - Microbiology 2020Quote: ... and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems) as described previously (21) ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.4) and quantified using the Qubit® DNA Assay Kit on a Qubit® 3.0 Fluorometer (Life Technologies, CA, USA). Alternatively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted DNA was suspended in Milli-Q water and concentration was determined with the Quant-iT dsDNA HS assay kit using a Qubit® 2.0 Fluorometer (Life Technologies). DNA integrity was assessed by electrophoresis ...
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bioRxiv - Genomics 2020Quote: ... We performed the DNA quality and quantity assessment using a Qubit double-stranded DNA HS Assay Kit (Invitrogen, Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... We performed the DNA quality and quantity assessment using a Qubit double-stranded DNA HS Assay Kit (Invitrogen, Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The concentration and integrity of total RNA were checked using the Qubit RNA Assay Kit in Qubit 4.0 Fluorometer (Life Technologies, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: The plasmid DNA yields from the sewage samples were evaluated using gel electrophoresis and Qubit double-stranded DNA (dsDNA) BR assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA). Plasmid DNA purity was measured and validated by absorbance ratio of 260/280 and 260/230 using NanoDrop 100 (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was visualized on a 1% agarose gel using Gel Red nucleic acid stain and quantified using Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and the NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... A portion (5 µl) was kept to quantify the total amount of proteins using Pierce™ BCA protein assay kit (23225, ThermoFisher). 5 ml of HNO3 5% (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 30 μL of supernatant was removed from the top of each well for measurement of LDH release using a Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Protein concentration of the extracts was estimated based on tissue weight or normalized using Pierce Coomassie Protein Assay Kit (ThermoFisher Scientific). Co-immunoprecipitation (coIP ...
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bioRxiv - Microbiology 2021Quote: ... The amount of total sterol in the EV samples was measured by the Amplex™ Red Cholesterol Assay Kit (ThermoFisher, A12216) and adjusted for the subsequent experiments.
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bioRxiv - Neuroscience 2020Quote: ... Total protein concentration was determined using the Bio-Rad Bradford protein assay kit according to the manufacturer’s instructions (ThermoFisher Scientific, #23225). An adapted BODIPY (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... gel purified and quantified using the Invitrogen™ Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). For all 2018 samples sequencing was performed using an Illumina MiSeq and the MiSeq Reagent Kit v2 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and fluorescent labeling was performed with the Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher), according to the manufacturer’s description ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100 with 100× protease inhibitors cocktail solution from GenDEPOT [P3100-005] and the phosphatase inhibitors NaF [10 mM] and Na3VO4 [20 mM]) and quantified using a bicinchoninic acid (BCA) protein assay kit (#23225, Thermo Scientific). The samples were added with 6X loading dye and boiled at 97 °C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were cleared by centrifugation and protein concentrations were determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of protein were diluted in Laemmli sample buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of protein per sample was determined using the Bicinchoninic Acid (BCA) Protein Assay Kit (23228, Pierce/Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... POS preparations were washed in PBS and resuspended in medium and protein content was measured (BCA Protein Assay Kit, Thermo Scientific) and in parallel ...