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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed using the QuantStudio 3 detection system (Applied Biosystems®) with the following steps ...
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bioRxiv - Biochemistry 2023Quote: ... Signal detection was accomplished using the SuperSignal West Pico Plus reagent (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed by Pierce™ ECL Western Blotting Substrate (Thermo Fisher).
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bioRxiv - Neuroscience 2024Quote: ... The arrays were incubated with a detection buffer with Cy5-streptavidin (ThermoFisher) at 1:1000 for 20 minutes at RT ...
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Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovanibioRxiv - Microbiology 2022Quote: ... Nitrite (NO2−)accumulation in the cell culture supernatants from all the subjects were measured as an indicator of NO production using a standard Griess reagent kit (Molecular Probes, USA; minimum detection level 1.0 μM).
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bioRxiv - Neuroscience 2020Quote: We conducted high-resolution metabolic profiling using liquid chromatography with ultra-high resolution mass spectrometry (LC-HRMS; Thermo Scientific Fusion) following established protocols[14] and published methods[15 ...
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bioRxiv - Bioengineering 2020Quote: ... Dex from the high Dex loading scaffolds (1% and 0.5% wt/wt) was measured by high-performance liquid chromatography (HPLC; Thermo Fisher) with an Acclaim 120 C18 3 µm 4.6×150 mm column ((Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were maintained at 37°C and 5% CO2 in high humidity and cultured in high glucose DMEM (Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2021Quote: ... Each cell line was grown in three different media: high-glucose medium (high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen 11995-065) containing 25 mM glucose ...
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bioRxiv - Immunology 2021Quote: ... Samples were analyzed by high-resolution/high-accuracy liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Lumos Fusion, ThermoFisher Scientific). MS and MS/MS were operated at resolutions of 60,000 and 30,000 ...
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bioRxiv - Neuroscience 2022Quote: ... A 11.2 kb PCR product that included a region from CLN3 exon 3 to exon 9 was amplified using a high fidelity polymerase (Platinum Taq High Fidelity, Invitrogen) and CLN3 primers pCLN3F3 and pCLN3R3 (Supplemental Table 2) ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 2 μg per sample was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 1000 ng of total RNA from each sample was used for the preparation of cDNAs in 20µl volume using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster City, CA). RT-qPCR amplification of cDNAs was carried out in a 10µl reaction mixture containing Applied Biosystems Power SYBR Green PCR Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: Each TMT labeled peptide set was fractionated to improve depth of proteome coverage using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific, Part 84868). The fractionation was performed according to the manufacturer’s protocol and yielded 8 peptide fractions for analysis ...
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bioRxiv - Immunology 2021Quote: ... Total RNA (1 μg) from each sample was used for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life Technologies). Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC) using an Fe-NTA matrix (High-Select™ Fe-NTA phosphopeptide enrichment kit, Thermo Scientific #A32992) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2μg of RNA was reverse transcribed into cDNA using a High-Capacity cDNA reverse transcription kit following the manufacturer’s instructions (4368814; Applied Biosystems, Foster City, CA). qRT-PCR was performed with Bullseye EvaGreen qPCR MasterMix (BEQPCR-S ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized from 500 ng of total RNA using a High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems by Thermo Fisher Scientific). For measurement of the mRNA levels of Arnt-2(Cat# Mm00476009_m1) ...
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Fetal estrogens are not involved in sex determination but critical for early ovarian differentiationbioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR was performed on reverse transcribed RNAs (High Capacity Reverse cDNA Transcription kit with the included set of random primers, Applied Biosystems, ThermoFisher, France). Several PCR sets of primers and analyses were those published previously (Daniel-Carlier et al ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using 500 ng of total RNA and the High-Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). QPCR was performed using the Wafergen SmartChip system (TakaraBio ...
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bioRxiv - Developmental Biology 2022Quote: Production of cDNA from RNA extracted from both bulk lungs and sorted cells was performed using the High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems™), following the manufacturers’ protocol ...
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bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... cDNA samples were barcoded using Platinum PCR SuperMix High Fidelity polymerase and Ion Xpress RNA-Seq Barcode 1–16 Kit (Life Technologies, Carlsbad, CA). Yield and size distribution of the cDNA libraries were assessed using a DNA1000 chip (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... After 24 hours the total RNA was extracted from each well and 1 μg of total RNA of primary microglial cells were reverse-transcribed using the High-Capacity cDNA Archive kit (Applied Biosystems, Carlsbad, USA), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... TMT labelled peptides were subjected to TiO2-based phosphopeptide enrichment according to the manufacturer’s instructions (High-Select™ TiO2 Phosphopeptide Enrichment Kit, Thermo Fisher Scientific, A32993). The flow-through and washes from the TiO2 enrichment step were combined ...
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A novel transplantable model of lung cancer associated tissue loss and disrupted muscle regenerationbioRxiv - Cancer Biology 2020Quote: ... Two μg of total RNA was reverse transcribed with primers using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Carlsbad, CA, USA). QPCR was performed on a ViiA7 Quantitative PCR System (Applied Biosystems by Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... as per the manufacturer’s instructions and used for bulk mRNA sequencing or converted to cDNA by reverse transcription with the High-Capacity RNA-to-cDNA Kit (Thermo Fisher, Waltham, MA) and utilized for quantitative real-time PCR analysis ...
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bioRxiv - Cell Biology 2019Quote: ... ~1 μg purified RNA was used to generate cDNA via High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat. No. 43-688-13). qRT-PCR was performed with TaqMan Universal Master Mix II ...
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bioRxiv - Physiology 2021Quote: ... The aforementioned tissues’ RNA was reverse transcribed to complementary DNA (cDNA) using Applied Biosystems’ High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA) and their recommended steps to make a 20 μL working solution ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and first-strand cDNA was synthesized from 1.5 µg total RNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: pJ6/JFH-1 Rluc plasmids were linearized by digesting with XbaI and Mungbean nuclease and invitro transcription was performed using MEGA Script T7 High Yield Transcription Kit (Life Technologies, Burlington, Canada). To generate T7 Fluc mRNA ...
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bioRxiv - Microbiology 2020Quote: ... or related mutants were linearized by digestion with XbaI and RNA was made by using the MEGA Script T7 High Yield Transcription Kit (Life Technologies, Burlington, Canada). The transcription process was performed using the suggested manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed using TaqMan gene expression assays after cDNA preparation using the high capacity reverse transcription kit (Applied Biosystems; cat#4368814). β-actin and tata-box binding protein (TBP ...
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bioRxiv - Cancer Biology 2020Quote: ... dried at 30°C in a vacuum concentrator and fractionated using a Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... agents following the manufacturer’s protocol and then qualified using a NanoDrop1000 spectrophotometer (Thermo Fisher Scientific, USA. The cDNA synthesis was carried out using a high Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). Taq Universal SYBR Green Supermix (BIO-RAD ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA and oligo primers were used for cDNA synthesis in a total reaction volume of 20 µL High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, 4368814). qRT-PCR reactions were prepared using SYBR-Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... One µg of RNA was reverse transcribed using the high-capacity cDNA reverse transcription kit following manufacturer’s recommendations (Applied Biosystems; Grand Island, NY). Standard Fast TaqMan PCR amplification was performed in triplicate on a Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Single-strand cDNA was made with 500 ng RNA using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, 4387406, Thermo Fisher Scientific). TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Single-strand cDNA was made with 500 ng RNA using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, 4387406, Thermo Fisher Scientific). TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: Reverse transcription was performed on 1□µg of total RNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific, Illkirch, France). cDNAs were amplified by PCR in a Roche real-time thermal cycler ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was quantified and cDNA synthesis was performed according to standard procedures using High-capacity cDNA Reverse Transcription Kit (4368813) (ThermoFisher, Waltham, MA, US). Real-time polymerase chain reaction (qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... 500ng of RNA was used to make cDNA using the Applied Biosystems High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific, cat# 4368813). qPCR was performed in a 20µL reaction using TaqMan™ Universal Master Mix II (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of cellular total RNA was reverse transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Cat# 4368813). qPCR was then performed using a QuantStudio 6 Flex Real-Time PCR Systems (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... Total left-over RNA from the RNAseq was used to synthetize cDNA with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA). A dilution 1/3 of the total cDNA was amplified by real-time PCR ...
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bioRxiv - Physiology 2020Quote: ... Complementary DNA was synthesized from 200ng of total RNA with the High-Capacity RNA-to-cDNA reverse transcription kit (Life Technologies, Waltham, MA). Real-time quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2020Quote: ... evaporated to dryness and subjected to Fe-NTA-based phosphopeptide enrichment according to the manufacturer’s instructions (High-Select™ Fe-NTA Phosphopeptide Enrichment Kit, Thermo Fisher Scientific, A32992). Phosphopeptides enriched using TiO2- and Fe-NTA-based methods were combined ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of input RNA were reverse-transcribed using Applied Biosystems High Capacity cDNA Archive Kit (Applied Biosystems Inc., Foster City, CA, USA). qRTPCR was conducted with TaqMan primers and reagents (Life Technologies Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (50 ng/μl) was converted to complementary DNA (cDNA) using the High Capacity cDNA Reverse Transcription kit (Catalog number: 468814, Applied Biosystems Inc. (ABI), Foster City ...
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bioRxiv - Cancer Biology 2020Quote: ... After the extraction the samples were submitted to a reverse transcription reaction using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, California, USA) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... RNA was measured using a Nanodrop 1 μg of RNA was then reverse transcribed into cDNA (Applied Biosystems, High Capacity cDNA kit, 4368813) with additional RNase inhibitor (Invitrogen RNase OUT ...