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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (Thermo Fisher Scientific; #31430 or #32460; 1:10,000).
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight in the primary antibodies diluted in the blocking solution then incubated sequentially with the secondary antibodies (donkey anti-rabbit Alexa Fluor 594 and donkey anti-goat Alexa Fluor 488, both Life technologies, conc. both 1:1000). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse, KPL, ref 072-03-18-06, 1/200; anti-rabbit AlexaFluor 488, Life Technologies, ref A21441, 1/200), slides were mounted in Vectashield mounting medium (Vector ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed to remove excess antibody and incubated in goat anti-mouse secondary antibody AF568 (Thermo Fischer Scientific, A-11004, 1:500) and goat anti-rabbit AF488 (Life technologies, A-11008, 1:500) for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an incubation in secondary fluorescent antibodies (1:500, Alexa Goat anti-Mouse IgG2b, A-21141, Alexa Goat anti-Rabbit IgG, A32733) and DAPI (Thermo Fisher Scientific; 62248, 1:500) in 0.1x PBS with 0.1% Triton X100 for 1 hour at 22-24°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by the addition of secondary antibodies in blocking buffer (goat-anti-rabbit-IgG-AlexaFluor488 [ThermoFisher Scientific #A11034] and goat-anti-mouse-IgG-AlexaFluor568 [ThermoFisher Scientific #A11031] both 1:200) and Hoechst 33342 (2 ug/mL from 10 mg/mL stock ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed and processed for immunostaining with mouse anti-VP2 (RT16.7) and secondary goat Alexa Fluor 488-conjugated anti-mouse antibodies (Thermo Fisher Scientific, cat. no. A-11029) as described2,71 ...
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bioRxiv - Biophysics 2022Quote: ... incubated with secondary antibodies (anti-mouse IgG, Alexa Fluor 488, superclonal [Thermo Fisher Scientific, Cat#A28175], anti-rabbit IgG, Alexa Fluor 568 [Thermo Fisher Scientific, Cat#A11036]) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... followed by binding of the primary antibody and a detection step facilitated by the incubation with Alexa Fluor 488-conjugated goat anti-rat or anti-mouse secondary antibody (Thermo Fisher Scientific, Waltham, MA, USA). Alexa Fluor 594-conjugated streptavidin was used (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with polyclonal Alexa Fluor 488-or 594-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Invitrogen Molecular Probes; Eugene, OR, USA). Alternatively ...
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bioRxiv - Physiology 2023Quote: ... samples were labeled with goat anti-mouse Alexa 647 (1:1000) and goat anti-rabbit Biotium CF 568 (1:2000) secondary antibodies (ThermoFisher Scientific, Grand Island, NY, USA). Simultaneous labeling with two primary antibodies from the same animal background was accomplished by completing the staining protocol with one set of primary and secondary antibodies followed by blocking with a donor secondary antibody (conjugated to fluorophore to assess degree of blocking ...
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bioRxiv - Systems Biology 2023Quote: ... samples were labeled with goat anti-mouse Alexa 647 (1:1000) and goat anti-rabbit Biotium CF 568 (1:2000) secondary antibodies (ThermoFisher Scientific, Grand Island, NY, USA). Where needed ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction obtained by centrifugation at 15,000 rpm for 15 min was reacted with sheep anti-mouse or anti-rabbit primary antibody-conjugated Dynabeads M280 (Thermo Fisher Scientific, Waltham, MA, USA) for 4 h or overnight ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies (A11007, Alexa Fluor™ 594, goat anti-rat IgG; A11073, Alexa Fluor™ 488, goat anti-guinea pig IgG; Invitrogen, Waltham, Massachusetts, USA) were diluted 1:250 in blocking buffer ...
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bioRxiv - Physiology 2024Quote: ... Slices were then incubated with secondary antibodies (Cy3 Goat anti-Mouse, Jackson immunoresearch, 115-165-146, 1:1000; Donkey anti-Rabbit, Invitrogen, A-21206, 1:1000) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were washed in PBS-T 3×5min and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA, USA) for 2 hours at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... add fluorescent secondary antibody (Goat anti-Mouse IgG, 1:500, Thermo Fisher Scientific # A-11001 or Goat anti-Rabbit IgG, 1:500, Thermo Fisher Scientific # A-11008) and incubate for 1 h at room temperature in a wet box ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500, Thermo Fisher Scientific #A-11004, #11008). The cultures were washed with PBS ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... and subsequently incubated with secondary antibodies (goat anti-chicken-FITC, Jackson #103-095-155, 1:500; goat anti-rabbit Alexa-Fluor-594, Thermo Fisher Scientific, A11012; 1:500) and DAPI (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies (goat anti-rabbit Alexa Fluor 488, Thermo Fisher Scientific, A11008, 1:2,000; goat anti-mouse Alexa Fluor 594, Thermo Fisher Scientific, A11032, 1:2,000) were diluted with TBS containing 0.2% Tween-20 and 3% BSA and incubated with coverslips for one hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred from the gel onto a nitrocellulose membrane using an electric current and fixed to the nitrocellulose and probed with antibodies against the target proteins (anti-PD and anti-ubiquitin; Invitrogen Ubi-1, Cat #13-1600). Bound antibody was revealed with horseradish peroxidase-conjugated rabbit anti-mouse antibody using enhanced chemiluminescence (Amersham Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... was used as a primary antibody and a goat anti-mouse IgG (H+L) cross-adsorbed antibody Alexa Fluor™ 647 (Thermo Fisher Scientific, A-21235; 1:500) was used as a secondary antibody ...
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Plant Biology 2020Quote: ... Western blot analysis was performed using anti-mCherry antibody (16D7) at 1:2000 dilution (Thermo Fisher, MA), anti–GFP antibody (GF28R ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-FLAG antibodies were cross-linked to Dynabeads® M-270 Epoxy (Thermo Fisher Scientific, cat# 14311D) for immunoprecipitation ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, ThermoFisher A21124 ...
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bioRxiv - Genomics 2021Quote: ... the slide was incubated with secondary antibody (1:25; Alexa Fluor 488 conjugated goat-anti-rabbit; Invitrogen, cat.no ...
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bioRxiv - Biophysics 2020Quote: ... and loading control for cell lysate detected α-tubulin using an anti-α-tubulin rabbit antibody (Invitrogen). Secondary detection was performed using Licor IRDye antibodies produced in goat (IRDye 680-labeled anti-rabbit and IRDye 800-labeled anti mouse).
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bioRxiv - Cancer Biology 2021Quote: ... mixed with 2 μL of anti-mouse CD16/CD32 antibody (cat. #14-0161-85, Thermo-Fisher Scientific), and incubated at 4°C for 20 min to block nonspecific IgG binding ...
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bioRxiv - Genomics 2021Quote: ... Cells were then treated with APC-conjugated F(ab’)2-goat anti-mouse secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... for immunofluorescence - a goat anti-mouse IgG (H+L) secondary antibody Alexa Fluor 555 (#A31572, Invitrogen, USA), a goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were washed and incubated with Alexa Fluor 488 goat anti-mouse antibody (Thermo Fisher; 1:100) for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-goat RFC1 antibody (2 μg/sample) coupled to Protein G agarose beads (Thermo Fisher Scientific) for 2 h on an end-over-end rotator at 4°C ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... GFP-Ist2 was detected with a rabbit polyclonal anti-GFP antibody (ThermoFisher ref. A11122, 1:5000 dilution), and Osh6-mCherry was detected with a rabbit polyclonal anti-RFP antibody (ThermoFisher ref ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used were F(ab’)2 goat anti-rabbit Alexa Fluor 568 (Molecular Probes, 1:1000), goat anti-mouse Alexa Fluor 488 (Molecular Probes 1:1000).
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bioRxiv - Cell Biology 2020Quote: ... Sections were then rinsed and stained with secondary antibody (biotinylated horse anti-mouse; Gibco; BA2000; 1:2000) for 1h at room temperature before rinsing and incubation with streptavidin-HRP (SNN1004 ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were donkey anti-goat IgG (H+L) Alexa Fluor 680 (1:10,000, Thermofisher, A-21804) and goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for these experiments: mouse anti-α-tubulin DM1α (1:500, Invitrogen 62204), rabbit anti-NuMA (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used for Western blotting were anti-rabbit Alexa Fluor 488/680/800 (Invitrogen, CA, USA), anti-rat Alexa Fluor 488/680 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... MuSCs were stained for 3 hours with 1:500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen) to detect cleaved caspase-3 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibody used was a goat anti mouse Alexa fluor 594 IgG (ThermoFisher Scientific, Cambridge, UK), at a concentration of 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa 488 or Alexa 594-conjugated goat anti-rabbit secondary antibody [1:400] (Invitrogen, Inc. Oregon, USA). Cells were incubated in ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies were highly cross-adsorbed Alexa 488-conjugated goat anti-mouse IgG (InVitrogen, ThermoFisher, Waltham, MA) and Alexa 594-conjugated goat anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies were highly cross-adsorbed Alexa 488-conjugated goat anti-mouse IgG (InVitrogen, ThermoFisher, Waltham, MA) and Alexa 594-conjugated goat anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies were Alexa-Fluor-488–coupled goat anti-rabbit (1:500; Life Technologies, Carlsbad, CA, USA). Gonads were counterstained with DAPI (0.1 μg/mL dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were incubated with secondary antibody (donkey anti-mouse Alexa Fluor 488, Thermo Fisher Scientific, 1:250) diluted in PBT + 1x WBR for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... 1× PBS) and subsequently with Alexa 594 linked goat anti-rabbit antibodies (1:250, Life Technologies, USA) as secondary antibody with intermittent washing ...
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-guinea pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 555 (Invitrogen #A21435) at 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 488 (Invitrogen #A32723) at 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... were used and developed using anti-goat IgG (H+L)-HRP conjugated antibodies (1:500 dilution, Invitrogen).