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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Invitrogen, 0.5 µL 12.5 mM dNTP mix, 1 µL 100 mM DTT, 1 µL SuperScriptIII reverse transcriptase, Invitrogen, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Genetics 2023Quote: ... ADH enzyme activity (units: ΔAbs340nm min−1 mL−1) and total protein (units: mg mL−1; Pierce BCA Assay, Thermo Fisher Scientific) of each sample were measured 3 times in technical replicates ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...
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bioRxiv - Neuroscience 2023Quote: Samples for lipidomics were resuspended in 25 µl chloroform/methanol (1:1) and 1 µl injected using a Vanquish Horizon UPLC (Thermo Fisher Scientific) equipped with a Waters ACQUITY Premier CSH (2.1 x 100mm ...
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bioRxiv - Cancer Biology 2024Quote: ... PC9 – CRUK0764 co-cultures were performed in a mixture of RPMI-1640 and DMEM (1:1) containing 1% fetal bovine serum (Gibco, #10270-106).
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bioRxiv - Immunology 2024Quote: ... resuspended in NK cell medium (RPMI 1640 medium containing GlutaMAX, 10% FBS, 1% P-S, 1% MEM Non-Essential Amino Acids Solution and 1% Sodium Pyruvate, both from Thermo Fisher Scientific), filtered using 70µm cell strainers and counted just before further use in flow cytometry experiments or in vitro experiments.
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bioRxiv - Neuroscience 2024Quote: ... The blot was then exposed to secondary antibodies (dilution 1:10,000 for Tuj-1 and 1:5000 for pan-Ago, Goat Anti-Mouse, Thermofisher Scientific, Catalog# A11030) for 3 hours ...
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bioRxiv - Systems Biology 2024Quote: ... prior to immunostaining according to standard protocols74 and using a primary antibody directed against zona occludens-1 (ZO-1; 1:100, Thermofisher Scientific, UK) or AF488-conjugated phalloidin (0.165 µM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM sodium pyruvate and 100 U mL−1 penicillin and 100 µg mL−1 streptomycin (all purchased from Thermo Fisher Scientific) at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Cell Biology 2024Quote: ... HIF1A (# NB100-479, Novus 1:500), HIF2A (# NB100-122, Novus, 1:500) while HRP-conjugated anti-rabbit IgG (# 31460, Invitrogen, 1: 10000) or anti-mouse IgG (# 62-6520 ...
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bioRxiv - Immunology 2024Quote: ... for one hour and incubated overnight with the primary antibodies (rabbit-anti-Iba-1, 1:200; Wako, USA; rat-anti- mCherry, 1:2000, Thermo Fisher Scientific). After washing in PBS ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595, 1:600; anti-GM130, Abcam, ab52649, 1:200; anti-dynein, Invitrogen, PA5-89505 ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were washed with 1x PBS twice for 5 minutes and incubated with DAPI (1 mg/ml, 1:100) and Alexa Fluor 488 phalloidin (1:1000, Thermo Scientific, A12379) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the whole medium was changed containing two parts E8 medium and one part neural differentiation medium (NDM) containing 1:1 DMEM F12 / Neurobasal medium supplemented with 1% B27 (Thermo Fisher, 12587010) 0.5% N2 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... mAb24B10 (DSHB, 1:50), anti-OLLAS (Funakoshi, 1:200) and goat anti-rat IgG(H+L) Alexa Flour 488 conjugate (Life Technologies, 1:400).
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with primary antibody (anti-ColX, 1:50, Abcam, ab58632, anti-NITEGE, 1:50, Thermo Scientific, PA1-1746, IgG (1:50) control ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of total RNA was treated with 1 μl of 10x DNase-I reaction buffer and 1 μl of DNase I Amp grade (1 U/μl; ThermoFisher, catalogue number 18068015), and then incubated for 15 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Kocks, 1:1000), anti-Srp (kind gift of A. Giangrande, 1:1000) and secondary antibodies conjugated to Alexa dyes (Molecular Probes, 1:500), fluorescently labeled phalloidin (Molecular Probes) ...
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bioRxiv - Immunology 2019Quote: ... then stained with secondary antibodies (donkey anti-rabbit DyLight 488, Abcam, 1:500; goat anti-guinea pig, Sigma, 1:500; anti-chicken Alexa 488, Life Technologies, 1:1000). Sections were mounted in VectaShield (Vector Labs ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing gastruloids were incubated with the appropriate secondary antibodies (Alexa Fluor DAR-594, 1:400, Molecular Probes #A21207; Alexa Fluor GAM-488, 1:400, Molecular Probes #A11001, Alexa Fluor DAG-594 1:400, Molecular Probes #). Cell nuclei were counterstained with DAPI (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... A staining buffer was prepared utilizing 3% BSA in 0.1% PBST and 10% of a 1% Tween 20 solution at a 1:25 or 1:50 concentration with a primary antibody [(DAPI, 1:2500 dilution, Thermo Fisher Scientific, D3571), (F-actin ...
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bioRxiv - Microbiology 2021Quote: ... incubated with 0.5xMIC of tamoxifen metabolites mixture and mixed in a solution of phosphate buffered saline containing Ethidium Homodimer-1 (EthD-1) (1:500) (Invitrogen, Carlsbad, CA, USA). After 10 min of incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in 1 mL the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000, Hoechst 33342 at 1:1000 [Thermofisher] and Phalloidin-AlexaFluor555 at 1:200 [Thermofisher] in blocking buffer). The tissue was washed extensively and then mounted on glass slides using VectaShield (Vector Labs ...
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bioRxiv - Immunology 2020Quote: ... The slices were blocked with 1% BSA/PBS for 1 h and stained with anti-Krt5 (Invitrogen cat. MA5-17057, 1:100 dilution), anti-PPP6C (Abcam cat ...
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bioRxiv - Plant Biology 2021Quote: ... A 0.5 g sample of the collected tissues were ground under liquid nitrogen and suspended in 1 ml of co-IP extraction buffer (1× PBS, 1% n-dodecyl-β-d-maltoside (Invitrogen, Carlsbad, CA), protease inhibitor cocktail cOmplete™ (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... NSCs were cultured in neural expansion medium (Advanced DMEM / F12 and Neurobasal medium 1:1 with 1% neural induction supplement) on a surface previously coated with Geltrex LDEV-Free (Thermo Fisher Scientific, A1413302) for expansion.
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bioRxiv - Neuroscience 2021Quote: ... but after rinsing sections were incubated in secondary antibodies (donkey anti-guinea pig 555 1:1,000 Sigma SAB4600298; goat anti-mouse 546 1:400 Invitrogen A-11030; goat anti-mouse 488 Invitrogen 1:400 A-11001) for three hours ...
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bioRxiv - Developmental Biology 2020Quote: ... antibodies were diluted 1:500 in PBT with 1% DMSO and 1% BSA and applied together with TO-PRO-3 (T3605, Thermo Fisher, Waltham, MA) diluted 1:1000 over- night at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... pahangi parasites were washed several times in warmed worm culture media (RPMI with 1% HEPES, 1% L-glutamine, 0.2% Penicillin/Streptomycin, and 1% w/v glucose [all Thermo Fisher Scientific, Waltham, MA]) and then counted and cultured at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed twice with 5ml of washing medium (Advanced DMEM/F12 supplemented with 1% Glutamax, 1% Penicilin/Streptomycin and 1% HEPES [1M, Gibco, cat no. 15630049]), centrifuging at 300g for 5 minutes (at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and HA were labeled with fluorescent secondary antibodies (donkey anti-rat IgG, 1:200; donkey anti-rabbit IgG, 1:200, Thermo-Fisher A-31573; anti-streptavidin IgG, 1:200, Invitrogen Molecular Probes S-32355), mounted onto glass slides ...
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bioRxiv - Genetics 2023Quote: ... neuronal rosetes were passaged as small clusters in either a 1:1 or 1:2 ratio using the StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool (Gibco, #23181010) or via hand-picking ...
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bioRxiv - Microbiology 2023Quote: ... After lysis in breaking buffer (20 mM potassium phosphate, 5 mM EDTA, 1 mg mL−1 lysozyme, 1 Pierce Protease Inhibitor Tablet (Thermo Scientific, Rockford, IL), 1 mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was washed three times in .05% PBST and HRP-conjugated goat anti-mouse secondary was added at 1:2000 for 1 hour (Invitrogen, 31430, 1:2000). U2OS cell western blot quantification was carried out using the ImageQuant™ software (Cytiva).
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bioRxiv - Cell Biology 2023Quote: ... The blot was washed three times in .05% PBST and HRP-conjugated goat anti-mouse secondary was added at 1:2000 for 1 hour (Invitrogen, 31430, 1:2000). U2OS cell western blot quantification was carried out using the ImageQuant™ software (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000, Invitrogen #P5-852912). After this period ...
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bioRxiv - Cell Biology 2023Quote: ... 1:200) for 1 h at RT and afterwards with a streptavidin eFluorTM 660 conjugate (#50-4317-80, Thermo Fisher Scientific, 1:20) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated with secondary antibody (1:750) and DAPI (1:500) or DR (Thermo Fisher Scientific, Cat#: 65-0880-92; 1:1000) nuclear stain in the blocking buffer for 2 days at 4°C and washed with PTW 3 times 10 minutes.
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x in PBS for 10 minutes at RT on shaker and incubated with Goat secondaries (1:1000 Invitrogen #A11036 568; 1:1000 Life Technologies #A110006 488; 1:1000 Invitrogen Channel 633.) diluted with 1%PBT for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... then in PBS supplemented with 1% BSA and 1% rabbit IgG blocking reagent (ThermoFisher) for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Basal bronchial epithelial growth medium was first diluted 1:1 with DMEM (Gibco; 41966). Next ...
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bioRxiv - Cell Biology 2020Quote: ... rhodamine-Wheat germ agglutinin (WGA, 1:400, Vector) and Hoechst (1:1000, Molecular Probes) for 30 min at RT and cover-slipped with Fluoromount (SouthernBiotech) ...
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bioRxiv - Immunology 2021Quote: ... 40uL of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) were added to each well and then incubated at 4ºC for 4 hours on a rotator ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Developmental Biology 2020Quote: ... 1% of Penicillin-Streptomycin and 1% of MEM Non-Essential Amino Acids (Gibco, U.S.). Cells were passed in T25 flasks at 37 °C with 5% CO2.
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.025 U/μL GoTaq polymerase enzyme and 1:66,000 SYBR Green-1 (Thermo Fisher). Clusterin (CLU ...
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bioRxiv - Molecular Biology 2020Quote: ... This was followed by a 1 mg ml−1 solution of NeutrAvidin (31000, ThermoFisher) for a further 5 minutes before being washed with PBS + 10 mM MgCl (immobilization buffer ...