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Citations for Thermo Fisher :
701 - 750 of 6996 citations for sCD4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 6x-His Tag Monoclonal Antibody (HIS.H8) (MA1-21315, Thermo Scientific) and HRP-conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG ...
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bioRxiv - Molecular Biology 2023Quote: ... with 5% heat inactivated Fetal bovine serum (HI-FBS) (Gibco). 1 million cells per 100 μl per sample were stained with anti-Flag antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LanthaScreen Elite Tb-anti-His antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... The medium was supplemented with 10% HI-FBS (Gibco, USA), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... bio-His-PADI4 was incubated with magnetic streptavidin beads (Invitrogen) (4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) in a CO2 incubation at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transiently transfected with plasmids using 293fectinTM Transfection Reagent (Cat: 12347019, Life Technologies) when the cell density reached 1 × 106 cells/mL ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Developmental Biology 2021Quote: HEK293 cells were transfected with myc or myc-MLLT11 vectors using Lipofectamine™ 2000 (Invitrogen). 24 hours later ...
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bioRxiv - Microbiology 2021Quote: ... Transfections of HEK293 were performed with 1.0 µg of expression vector using Lipofectamine 2000 (ThermoFisher). After 12 to 30 h of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... U2OS and HEK293 cells were purchased from ATCC cell repository and cultured in DMEM (Gibco), supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 and SH-SY5Y cells were transfected with 25 nM siSARM (s23032, Ambion, life technologies) for 48 h followed by 24 h rotenone treatment ...
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bioRxiv - Genetics 2019Quote: HEK293 cells used for indel acquisition assays were transfected with Liopofectamine 3000 (Thermo Fisher #L3000008) according to manufacturer’s protocol with 5ug of ErCas12a plasmid targeting each site ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM L-glutamine) and used to infect 80-100 % confluent HEK293-TRex cells (ThermoFisher) grown in 96-well plates (BD Purecoat Amine ...
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bioRxiv - Immunology 2019Quote: ... HEK293 T cells were transfected in 12-well plates with Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... For expression in the HEK293 derived Expi293F™ cells (Thermo-Fisher Scientific, Cat. No. A14527), full-length ZMPSTE24 gene was cloned into the pHTBV1.1-LIC baculovirus transfer vector (The BacMam vector backbone ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 cell line were seeded and treated with Lipofectamine 3000 (Thermo Scientific, Waltham, MA, USA) and 1µg/ml DNA in OptiMEM reduced-serum medium (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells and Mouse Embryonic Fibroblasts (MEFs) were grown in DMEM (11965-092, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 and HeLa cells were cultured at 37°C with 5% CO2 in DMEM (Gibco) with 10% heat-inactivated FBS (BioInd) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were plated in 6-well plates and maintained in high glucose DMEM (Invitrogen) growth media supplemented with 1% pen/strep (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 10% (HEK293) or 12% (C17.2 or SHSY5Y) of newborn calf serum (Thermo Fisher Scientific). Endogenous TH were depleted from the serum by stripping with activated charcoal (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cells (1×105) were transfected into HEK293 with 1µg plasmid DNA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... removed supernatant and resuspended cells in serum free medium HEK293-SFMII (Gibco, catalog #: 11686-029), supplemented with 25 mM HEPES (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were plated in a 15-cm dish in 25ml of DMEM/F12 (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Mouse Embryonic Fibroblast (MEFs) and HEK293 (ATCC) cells were maintained in the DMEM medium (Gibco) containing glucose 1 g/L ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (10566-016, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: All transfections in HEK293 and CTX0E16 hNPCs were carried out using Lipofectamine 2000 (Invitrogen, 17504044), per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... whereas HeLa FRT and HEK293 cells were transfected using Opti-MEM and Lipofectamine RNAiMAX (Invitrogen). The following siRNAs were used ...
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bioRxiv - Immunology 2020Quote: ... transfected and sorted HEK293s were incubated with 1 μM LysoSensor Green DND-189 (Life Technologies) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured under standard conditions (37°C, 5% CO2) in DMEM medium (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were maintained at 5% CO2 at 37°C in DMEM with glutamine (Invitrogen) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Biophysics 2021Quote: ... HeLa and HEK293 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Catalog number: 11885084). All culture media were supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1 ...
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bioRxiv - Immunology 2021Quote: ... 2 × 106 CHO-hCD4 or HEK293-hCD4 were non-enzymatically detached using dissociation buffer (Gibco) and suspended in 5% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Transfection of plasmid vectors to HEK293 cells was conducted using Lipofectamine 2000 (Thermo Fisher Scientific) and Thermo Fisher Scientific’s recommended protocols for transfection using Lipofectamine 2000 ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were obtained from ATCC and maintained in DMEM (Gibco, Cat no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Immunology 2022Quote: ... HeLa cells and HEK293 were grown in high glucose DMEM (ThermoFisher, 11995-065; Biochrom, F0435) containing 2 mM L-glutamine (ThermoFisher ...
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bioRxiv - Synthetic Biology 2022Quote: L929 mouse fibroblast cells (ATCC# CCL-1) and HEK293 cells were cultured in DMEM (Invitrogen) containing 10% fetal bovine serum (Laguna Scientific ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected in 6 well plates at 60% confluence using lipofectamine LTX (Invitrogen) with 1µg of each transposon vector along with 0.5µg of pCMV-m7PB hyperactive transposase (34) ...
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bioRxiv - Bioengineering 2024Quote: ... All other CYpHER molecules were produced by transient expression in suspension HEK293 cells (ThermoFisher GeneArt) and purified either via Ni-NTA pull-down as previously described (33 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293/CRE-Luc/hCXCR4 cells were plated in Opti-MEM media (Invitrogen, cat# 31985-070) supplemented with 0.1% FBS (Seradigm ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were co-transfected with 200 ng of GeneArt CRISPR Nuclease mRNA (Invitrogen #A29378) in addition to sgRNA prepared by mixing fluorescently labelled Alt-R CRISPR-Cas9 tracrRNA ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were incubated for 1.5 hours with glutamate-free DMEM GlutaMAX-I (Life Technologies), to reduce the extracellular concentration of glutamate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen). The cells were replated 2 days after transfection and treated with 1 mg/mL G418 sulfate (EMD Chemicals Inc. ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1 ...
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bioRxiv - Biophysics 2023Quote: ... The construct was transfected in HEK293 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA #11668019), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells were grown and maintained in high-glucose (4.5 g/l) DMEM (Thermo Fisher) for BRET assays ...