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Citations for Thermo Fisher :
701 - 750 of 5842 citations for Serpin B6 Human Trx His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... human serum albumin (Fisher Scientific, OSRHSA10), pooled human plasma (Innovative Research ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml human bFGF (Invitrogen), and 50 ng/ml recombinant human FGF-10 protein ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney cells (HEK293FT, Invitrogen) were maintained in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Genomics 2024Quote: ... for human ACTB (Applied Biosystems, #Hs01060665_g1), and TGF-β1 (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... goat anti-human IgG antibodies (Invitrogen) were added and incubated for 1Lh ...
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bioRxiv - Immunology 2024Quote: ... human IL-1beta (Invitrogen™ 88726177) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... human IL-8 (Invitrogen™ 88808677), human IL-1beta (Invitrogen™ 88726177 ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF (Gibco) + 25 ng/ml human FGF10 (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... anti-human CCR7 (clone 3D12, Invitrogen), streptavidin (BioLegend) ...
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bioRxiv - Biophysics 2023Quote: ... 5 µg/mL human insulin (Gibco), 2 mM L-glutamine (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples containing the extension products were added to Hi-Di formamide (Applied Biosystems, Foster City, CA). The mixture was incubated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... Air-liquid interface medium consisted of 50% BEGM and 50% hi-glucose minimal essential medium (DMEM, Gibco) containing 100 nM additional retinoic acid (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus POR1 genomic DNA and inserted into the multiple cloning site (MCS) of pBAD/Myc-His (Invitrogen) vector containing a kanamycin-resistance cassette 25 (in-frame or out-of-frame with the C-terminal Myc-His tag ...
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bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
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bioRxiv - Developmental Biology 2021Quote: ... The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat. No. K4800-01, Invitrogen). HEK293T cells were cultured in DMEM medium with 10% FBS in 5% CO2 at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The araC gene and araBAD promoter were PCR-amplified from pBAD/His/lacZ (Invitrogen, Carlsbad, CA, USA). The lac promoter and inhibitory unit (iUnit ...
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bioRxiv - Developmental Biology 2020Quote: ... The template was prepared using the Ion PI Hi-Q OT2 200 kit (Thermo Fisher Scientific, UK). Sequencing was performed using the Ion PI Chip Kit v3 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Bioengineering 2019Quote: ... and cloned into the standard mammalian expression vector pEF1/myc-His B (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2019Quote: His-tagged SIVsmm Nef constructs were expressed in BL21 (DE3) star cells (Life technologies, Grand Island, NY), and induced with 0.3 mM IPTG at 25 °C overnight ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Cell Biology 2020Quote: ... The protease was removed using His-Pur™ Ni-NTA agarose resin (Thermo Fisher Scientific, Waltham, MA). Gle1152-360 was further purified by a 30% ammonium sulfate precipitation ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293T cells were cultured in DMEM (HyClone) supplemented with 10% heat-inactivated FBS (HI-FBS; ThermoFisher). CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The stable cell lines were generated using the pcDNA/FRT/V5-His TOPO TA Expression kit (Invitrogen) performed according to the manufacturer’s protocol and as described previously (28) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Microbiology 2021Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in Express-Five media (Life Technologies) and infected with 0,5% vol/vol of generation 2 (V1 ...
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bioRxiv - Neuroscience 2020Quote: ... CCM2 isoforms were inserted into pcDNA3.1/V5-His-TOPO and Topo pCR®II plasmid vectors (Invitrogen) respectively (1-6 ...
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bioRxiv - Molecular Biology 2020Quote: The sequence of the OpIE2 promoter was obtained from pIB/v5-His map sequence (ThermoFisher Scientific, USA). The truncated version of the wild-type OpIE2 promoter sequence (tIE2) ...
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bioRxiv - Microbiology 2020Quote: ... The templates were sequenced with an Ion PGM Hi-Q View Sequencing Kit (Thermo Fisher Scientific Inc.) and a 318 Chip Kit v2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Immunology 2022Quote: For purification of the fusion proteins by His Pur™ Ni-NTA Resin (Thermo Scientific, Beijing, USA), the 250 mL IPTG-induced bacterial solution was centrifuged at 4 °C at 12000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Biochemistry 2022Quote: ... and AcGFP1 DNA fragments were synthesized (Gene Universal) and inserted into pAc5.1/V5-His A vector (Invitrogen). The FUS-Venus plasmid was a kind gift from Dr ...
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bioRxiv - Microbiology 2021Quote: ... then cloned into the BglII and XhoI sites of the pMT/BiP/V5-His vector (Invitrogen, #V413020). The recombinant protein was expressed and purified using the Drosophila Expression System as described previously (Schuijt et al. ...
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bioRxiv - Microbiology 2021Quote: ... purified His-RBD was diluted to 200 μg/mL in PBS buffer containing Sypro Orange 5× (ThermoFisher) in a 96-well white PCR plate ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP2α was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Pathology 2021Quote: ... For expression in eukaryotic cells the pcDNA™ 3.1/V5-His TOPO® TA Expression Kit (Invitrogen) was used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Templating was performed using the Ion PI™ Hi-Q™ OT2 200 Kit (Thermo Fisher Scientific), followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in Hi-Di formamide supplemented with R0X350 dye standards (1:8 ROXF) (Thermo Fisher Scientific). 1:3 and 1:15 dilutions of the samples in ROXF were analyzed by capillary electrophoresis (ELIM Biopharmaceuticals).
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bioRxiv - Cell Biology 2020Quote: ... the STIM1 cDNA was cloned into the pcDNA3.1/V5-His TOPO TA vector (Invitrogen, Cergy-Pontoise, France). All STIM1 variants were generated from the wild-type (WT ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Cell Biology 2020Quote: ... His-ubiquitin conjugates were eluted by boiling resin in 2x LDS sample buffer (Thermo Fisher Scientific, NP0008) supplemented with 200 mM DTT and 200 mM imidazole HCl ...
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat ...
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bioRxiv - Biophysics 2021Quote: ... reaction with the PCR product and an XhoI/HindIII-digested pBAD/His-B vector (Thermo Fisher Scientific). Using pBAD-GCaMP6s as the template ...
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...