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Citations for Thermo Fisher :
701 - 750 of 7414 citations for Recombinant Human RPE His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody against His (631212, Fisher Scientific) is commercially available ...
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bioRxiv - Neuroscience 2023Quote: The plasmid pcDNA6.1/V5-His B (Thermofisher) was used as the template to subclone in the full-length PNPLA6 cDNA (from RefSeq transcript NM_001166111.1) ...
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bioRxiv - Microbiology 2023Quote: ... and probed with □-His mAb (Thermo Scientific) diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant (Invitrogen, no. 10342-020). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant β-lactamase (ThermoFisher) in PBS (0.15 nM ...
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bioRxiv - Physiology 2021Quote: ... Recombinant CSF1R (Invitrogen, Carlsbad, CA) was used at a concentration of 2.5 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% mouse recombinant EGF (Invitrogen, 5% Recombinant human R-spondin (Peprotech).
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bioRxiv - Cancer Biology 2024Quote: ... recombinant (Thermo Fisher Scientific, #10342020), and specific oligonucleotides (Supplementary Table S4 ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Cell Biology 2021Quote: ... AnkB440 KO primary cortical neurons were transfected with Halo-tagged AnkB440 or GFP-tagged AnkB220 plasmids at DIV0 by lipofection (Lipofectamine 2000, Thermo Fisher Scientific). After brain dissociation ...
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bioRxiv - Immunology 2022Quote: ... of the different constructs were ordered and cloned into a pPPI4 avidin-tagged and/or hexahistidine-tagged vector by PstI-BamHI digestion and ligation with Gibson Assembly (Thermo Fisher Scientific). The recombinant human ACE-2 receptor was obtained in the same way after ordering the corresponding gBlock gene fragment (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... The secondary antibodies tagged with Alexa Fluor 647 (Invitrogen) at 5 μg/mL were added to the slices and incubated for 1 h before three-times washing and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... or Alexa647-tagged antisera (1:500; Invitrogen, Karlsruhe, Germany) in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... tagged with Alexa Fluor 594 or 488 dyes (Invitrogen), and the signal enzymatically augmented with sequential tyramide signal amplification (TSA amplification kits ...
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bioRxiv - Physiology 2020Quote: ... Amp-3 and HRP-tagged probe (Thermo Fisher Inc), one drop each for 30 ...
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bioRxiv - Immunology 2021Quote: ... and Rhodamine or Alexa Fluor 488-tagged Phalloidin (Invitrogen) were used for counterstaining cells ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies and detected using fluorescently tagged secondary antibodies (Invitrogen, goat raised anti-mouse 488 and Anti-rabbit 555) ...
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bioRxiv - Molecular Biology 2023Quote: ... Whi5 tagged with GFP was obtained from Thermo Fisher Scientific GFP yeast clone collection ...
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bioRxiv - Systems Biology 2019Quote: The RPE cells generated from hESCs were cultured in α-MEM medium (Life Technologies, Grand Island, NY, USA) containing 1% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Cell Biology 2022Quote: 40 ug of RPE cell lysates were separated through SDS-PAGE on a 4–12% NuPAGE gel (Invitrogen) and transferred to an Immobilon-FL polyvinylidene fluoride membrane (IPFL00010 ...
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bioRxiv - Developmental Biology 2021Quote: ... RPE was isolated from sections using IR capture and UV dissection on CapSure HS LCM Caps (ThermoFisher Scientific), and total RNA extraction was performed using PicoPure RNA Isolation Kit (Arcturus ...
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bioRxiv - Biochemistry 2019Quote: ... RPE proteins were loaded into precast 4-12% NuPage Bis-Tris gels (Thermo Fisher/Life Tech, Carlsbad, CA) and resolved with PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... The hTERT- immortalized retinal pigment epithelial cell line hTERT RPE-1 cell were grown in DMEM/F12(Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: All hTERT RPE-1 cell lines were cultivated in DMEM/F12 cultivation media (Thermo Fisher Scientific, Cat.N.31331028), supplemented by 10% fetal bovine serum (Biosera) ...
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bioRxiv - Cancer Biology 2023Quote: ... RPE-1-hTERT p53-WT cells stably expressing Cas9 endonuclease were reverse transfected (RNAiMAX reagent, Thermo Fisher Scientific) with the Alt-R CRISPR-Cas9-crRNA complexed with Alt-R CRISPR-Cas9-tracrRNA–ATTO550 (final concentration of 10nM) ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...