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Citations for Thermo Fisher :
701 - 750 of 5759 citations for Recombinant Human IL2RB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the nucleotide sequence was inserted by means of a BP recombinant reaction (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Recombinant GST and GST-tagged EB1 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.7 and induced with 1 mM IPTG for 5.5 h at 25°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant lentiviruses were produced by transient transfection in HEK293FT cells (Invitrogen, CA, USA), as described earlier 52 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were generated using the Bac-to-Bac Baculovirus Expression system (Invitrogen). Sf9 cells were cultured in Sf-900 III SFM medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant viruses were generated using the Bac-to-Bac Baculovirus expression system (Invitrogen). The proteins were expressed by infecting 50 ml of Sf9 insect cells at density of 1.0 x 106 ml−1 with the above-described viruses ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were generated using the Bac-to-Bac baculovirus expression system (Invitrogen) and the proteins were expressed in Sf9 insect cells infected at a density of 1.2 x 106 ml−1 with P3 baculovirus stocks in a 1:1 (v/v ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Biochemistry 2021Quote: Recombinant proteins were labeled with Alexa Fluor™ 647 NHS ester (Thermo Fisher) as described previously.20 In brief ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM Pierce™ recombinant protein A (#21184, Thermo Fisher Scientific, Waltham, USA) 50 nM bovine serum albumin (#A4612 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was removed with recombinant DNase (TURBO DNA-free™ Kit, Ambion). Concentration and purity of RNA were both evaluated with NanoDrop spectrophotometer ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells for recombinant antigen and monoclonal antibody production (Thermo Fisher Scientific, A1452) were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... and ligated to produce recombinant constructs using T4 DNA ligase (Thermo Fisher Scientific). Ligation reactions contained 50 ng of vector ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Neuroscience 2022Quote: ... recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... Recombinant plasmids were transfected into cell lines using Lipofectamine 2000 (Invitrogen; cat.no: 11668019). Vector backbone and unmethylated constructs were used as internal controls for comparison ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant T-REx-HEK-CFTR cells were generated by lipofectamine transfection (Life Technologies) of plasmids containing mutated versions of CFTR (RefSeq NM_000492) ...
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Neuroscience 2024Quote: For recombinant lentivirus production - HEK293FT cell line (cat# R70007, ThermoFisher Scientific, Auckland, NZ), HT1080 cell line (cat# CCL-121 ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocyte growth supplement (HMGS, Gibco), gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Pool A (human) (Thermo Fisher Scientific) using the TaqMan® microRNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... human skeletal myoblasts (Thermo Fisher Scientific), and mouse C3H muscle myoblasts (C2C12 cells ...