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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human FOLR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... Biotinylated surface proteins were then isolated with neutravidin-coated agarose beads (Thermo Fisher Scientific, Darmstadt Germany). The isolates were boiled at 95 °C for 7 min in the presence (reducing ...
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bioRxiv - Biochemistry 2021Quote: Biotinylated proteins from BioID experiments were purified for proteomic analysis with Dynabeads MyOne Streptavidin C1 (Invitrogen) as previously described [31] ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were dialyzed into bicarbonate buffer and biotinylated using EZ-LinkTM Sulfo-NHS-LC-Biotin (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Biotinylated SARS-CoV-2 S1 and RBD proteins were combined with fluorescent NeutrAvidin beads (Life Technologies) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... biotinylated-S1B/RBD proteins were captured on high-capacity streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were captured on 100 µl of packed high capacity NeutrAvidin sepharose beads (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were isolated by streptavidin pull-down (SA-Pd) using SA-coated magnetic beads (Invitrogen). The biotin-labeled proteins were separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... lysed and subjected to a pull-down assay of biotinylated proteins with neutravidin-agarose (Thermo Scientific) as previously described (Roux et al ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with 30 min additional incubation with Streptavidin-Alexa 647 (Thermo Fisher Scientific). Membranes were washed four times in PBS and imaged using the Odyssey Imaging System.
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bioRxiv - Microbiology 2023Quote: ... the biotinylated proteins were visualized using SuperSignal Western blot FEMTO substrate (Thermo Scientific, Cat. No. 34094).
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bioRxiv - Microbiology 2023Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific) as reported previously 67.
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bioRxiv - Biochemistry 2023Quote: ... Smurf1 HECT NT protein was biotinylated using the No-Weight NHS-PEO4-Biotin kit (Thermo Fisher) due to its compatibility with Super Streptavidin (SSA ...
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bioRxiv - Biochemistry 2024Quote: ... samples were cleaned from endogenously biotinylated proteins using Pierce™ NeutrAvidin™ Agarose beads (Thermofisher, #29200) and precipitated ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected by incubating samples in Alexa Fluor 568 conjugated streptavidin (1:1000; ThermoFisher) diluted in blocking solution for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/mL sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) on ice for 45 minutes with gentle shaking ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Immunology 2022Quote: The Invitrogen ProtoArray® Human Protein Arrays (ThermoFisher Scientific) are high-density microarrays that contain more than 9,000 unique human proteins individually purified and arrayed onto a nitrocellulose-coated slide ...
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Epitope-tagged proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher). Anti-D-cysteine immunoprecipitates were eluted by incubating beads in 1 mM D-cys in TBS for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged ARL15 proteins were immunoprecipitated using 50 μL of Pierce Anti-DYKDDDDK Magnetic Agarose (ThermoFisher Scientific) for 2 hours at 4 ºC ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
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bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant N proteins were coated on 96 well flatbottom immunosorbent plates (Nunc Immuno MaxiSorp) at a concentration of 500 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: Purified recombinant proteins were labelled using amine reactive NHS ester dyes (Thermo Fisher Scientific). Alexa fluorophores used to label proteins include ...
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bioRxiv - Immunology 2022Quote: Recombinant proteins were produced using the Freestyle™ 293 Expression System (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... All the recombinant proteins were transformed and expressed in E.coli BL21 (DE3) (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... Recombinant BA.5 and BQ.1.1 RBD proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was resolved by NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen; NP0322BOX) to determine identity and purity ...
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bioRxiv - Biochemistry 2023Quote: The following recombinant proteins were purchased from commercial sources: alkaline phosphatase (Thermo Scientific #EF0651), Antarctic phosphatase (New England BioLabs ...
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bioRxiv - Immunology 2023Quote: ... and recombinant Cas9 protein via electroporation using the Neon transfection system (Thermo Fisher Scientific). The following day ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific) and amylose resin (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Biophysics 2020Quote: ... Biotinylated CD3 (OKT3, ThermoFisher) and CD28 (CD28.2 ...
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bioRxiv - Biophysics 2023Quote: ... Biotinylated-BSA (Thermo Fisher) was diluted in ultrapure water to a concentration of 0.2 wt% and diluted in PBS to a final concentration of 0.1 wt% ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...