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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human EPCAM Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
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bioRxiv - Cancer Biology 2022Quote: ... EPCAM CD326-FITC (Invitrogen, clone VU-1D9, Cat. #: A15755, 1:100), CD49f-PE-Cy7 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... EpCam-APC (1:500, Thermo Fisher Scientific Cat# 17-5791-82); cKit-PE (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Bronchial epithelial cells were stained with EpCAM (Ebioscience/Thermo Fisher Scientific) and quantification of mitochondria transferred to epithelial cells was done by gating double positive cells for EpCAM-PE and Mito-GFP in FACS dot plot on FL3 window.
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bioRxiv - Cancer Biology 2023Quote: ... and APC anti-EpCAM (Invitrogen, Clone G8.8, Cat# 17-5791-80) at 1:100 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... 30 μg of biotinylated protein were mixed with 60 μL of streptavidin agarose resin (Thermo Fisher) and rotated (end-over-end ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... Biotinylated surface proteins were then isolated with neutravidin-coated agarose beads (Thermo Fisher Scientific, Darmstadt Germany). The isolates were boiled at 95 °C for 7 min in the presence (reducing ...
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bioRxiv - Biochemistry 2021Quote: Biotinylated proteins from BioID experiments were purified for proteomic analysis with Dynabeads MyOne Streptavidin C1 (Invitrogen) as previously described [31] ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were dialyzed into bicarbonate buffer and biotinylated using EZ-LinkTM Sulfo-NHS-LC-Biotin (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Biotinylated SARS-CoV-2 S1 and RBD proteins were combined with fluorescent NeutrAvidin beads (Life Technologies) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... biotinylated-S1B/RBD proteins were captured on high-capacity streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were captured on 100 µl of packed high capacity NeutrAvidin sepharose beads (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... the biotinylated proteins were visualized using SuperSignal Western blot FEMTO substrate (Thermo Scientific, Cat. No. 34094).
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bioRxiv - Cell Biology 2023Quote: ... lysed and subjected to a pull-down assay of biotinylated proteins with neutravidin-agarose (Thermo Scientific) as previously described (Roux et al ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with 30 min additional incubation with Streptavidin-Alexa 647 (Thermo Fisher Scientific). Membranes were washed four times in PBS and imaged using the Odyssey Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were isolated by streptavidin pull-down (SA-Pd) using SA-coated magnetic beads (Invitrogen). The biotin-labeled proteins were separated by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific) as reported previously 67.
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected by incubating samples in Alexa Fluor 568 conjugated streptavidin (1:1000; ThermoFisher) diluted in blocking solution for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... Smurf1 HECT NT protein was biotinylated using the No-Weight NHS-PEO4-Biotin kit (Thermo Fisher) due to its compatibility with Super Streptavidin (SSA ...
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bioRxiv - Biochemistry 2024Quote: ... samples were cleaned from endogenously biotinylated proteins using Pierce™ NeutrAvidin™ Agarose beads (Thermofisher, #29200) and precipitated ...
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bioRxiv - Microbiology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/mL sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) on ice for 45 minutes with gentle shaking ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
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bioRxiv - Immunology 2022Quote: The Invitrogen ProtoArray® Human Protein Arrays (ThermoFisher Scientific) are high-density microarrays that contain more than 9,000 unique human proteins individually purified and arrayed onto a nitrocellulose-coated slide ...
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Epitope-tagged proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher). Anti-D-cysteine immunoprecipitates were eluted by incubating beads in 1 mM D-cys in TBS for 30 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged ARL15 proteins were immunoprecipitated using 50 μL of Pierce Anti-DYKDDDDK Magnetic Agarose (ThermoFisher Scientific) for 2 hours at 4 ºC ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
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bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant N proteins were coated on 96 well flatbottom immunosorbent plates (Nunc Immuno MaxiSorp) at a concentration of 500 ng/mL ...