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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human EGFR protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Purified RBD protein (100 ug) was labeled with Alexa Fluor 488 using microscale protein labeling kit (Life Technologies, #A30006) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... CD117-APC (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... CD4 APC (Invitrogen), CD4 APCCy7 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... SlamF6-APC (Invitrogen), CD127-Superbright 436 (eBioscience) ...
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bioRxiv - Microbiology 2020Quote: ... labeled and hybridized to separate arrays using the GeneChip Human Exon 1.0 ST Array (Affymetrix, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Each gel included a migration standard comprised of Di-I labeled human LDL (L3482, ThermoFisher Scientific) that was diluted in cryoprotectant and stored in frozen aliquots (see recipes) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Microbiology 2024Quote: Intact VHH-Fc proteins were separated on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) online connected to an LTQ Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with primary antibodies against EGFR (mouse monoclonal, Life Technologies) and sortilin (rabbit polyclonal ...
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bioRxiv - Cell Biology 2023Quote: Peptides (PET1, TM-EGFR, pHLIP, and Scrambled) were synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Cell Biology 2023Quote: CEM were counted and resuspended at a concentration of 2-3 x 106 cells/mL in ice cold FACS buffer (PBS, 10% FCS, 2mM EDTA) containing 1.25 µL anti-human CD71 (0.625 µg/mL, OKT9, Invitrogen) for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck) ...
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bioRxiv - Microbiology 2021Quote: ... CD49b (clone DX5, APC-Cy7, Biolegend or APC-e780, Affymetrix Inc) and MHC-II (clone M5/114.15.2 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed in PBSE-d and labeled with a goat anti-rabbit APC conjugated secondary antibody (1:500 dilution, ThermoFisher Scientific, cat # 31984) and DAPI (1:5000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) and B-cell (CD19-eFluor450, ThermoFisher Scientific, Grand Island, NY) markers after blocking with anti-mouse CD16/32 antibodies (BioLegend) ...
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bioRxiv - Biochemistry 2020Quote: Both untagged and His-tagged EncFtn proteins were quantified using the Pierce BCA Assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions for the Test-tube procedure ...
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bioRxiv - Biochemistry 2020Quote: ... Uncleaved His-tagged proteins were removed by incubation of the sample with 1 ml HisPur™ (Thermo Fisher) nickel resin for 1h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 10 μM protein with various concentrations of DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescently labeled proteins were detected using an iBright FL1000 imaging system (Invitrogen, CA, USA). The Western blot results were quantified using ImageJ software (National Institutes of Health ...
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bioRxiv - Cell Biology 2022Quote: ... was fluorescently labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested and TMT labeled using the TMTduplex isobaric mass tagging kit (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... or Alexa-494 gelatine (labeled with molecular probes protein labeling kit, Thermo Fisher Scientific) to make the spot boundaries visible ...