Labshake search
Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... To compare protein quantity between the medulla and cortex a standard method described in the Taqman Protein Assays Sample Prep and Assay (Thermo Fisher, 4453745) was used ...
-
bioRxiv - Cell Biology 2021Quote: ... and by comparing band intensities of purified protein samples with a dilution series of 2 mg/mL Bovine Serum Albumin standard (Thermo Fisher 23209) on a Coomassie-stained SDS-PAGE gel ...
-
bioRxiv - Immunology 2021Quote: CHLA255 or KCNR tumor cells expressing green fluorescence protein and luciferase were expanded under standard culture conditions (described above) and harvested with Trypsin (Gibco, Thermo Fisher Scientific). For the orthotopic experiments 1e6 KCNR-GFP Luc or 1e6 CHLA255-GFP Luc tumor cells were implanted into the renal capsule as previously descibed57 ...
-
bioRxiv - Cell Biology 2019Quote: ... The concentration of purified protein was determined by Coomassie staining of 14 % gels after SDS-PAGE with BSA standards obtained from the Pierce BCA protein assay kit (23209, Thermo Fisher Scientific). The purified BZLF1 protein was used as a quantitative standard and reference for subsequent Western blot analyses.
-
bioRxiv - Biochemistry 2021Quote: ... CLPTM1L-FH proteins and BSA standards (100ng, 200ng, 500ng, and 1000ng) were separated on SDS-PAGE followed by Imperial Protein Stain (24615, Thermo Fisher Scientific) to quantify the concentration of CLPTM1L-FH ...
-
bioRxiv - Genetics 2022Quote: ... Co-purified proteins were identified using standard protocols utilizing on bead-digested sample evaluated on a Q Exactive mass spectrometer (Thermo Fisher Scientific) (Van Leene et al ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 21130 g for 10 min at 4°C and quantified for protein concentration using an albumin standard (Thermo Fisher 23209) and Bradford reagent (Bio-Rad 5000006) ...
-
bioRxiv - Plant Biology 2022Quote: ... tauri cell lysates were run on a Novex NuPAGE 4-12% Bis-Tris by SDS-PAGE with PeppermintStick Phosphoprotein Molecular Weight Standards and Spectra Multicolor Broad Range Protein Ladder (Thermo Fisher Scientific). The gel was fixed overnight (50% methanol ...
-
bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 14,000xg for 15 minutes at 4°C and protein concentrations were determined using a standard Pierce™ bicinchoninic acid (BCA) assay (ThermoFisher, 23225). Lysate (30 µg ...
-
bioRxiv - Neuroscience 2023Quote: ... the protein lysates were thawed and the total protein concentration in each lysate was estimated by bicinchoninic acid (BCA) assay using bovine serum albumin standards (Thermo Scientific, UK) as described by Sekhar et al. ...
-
bioRxiv - Plant Biology 2024Quote: ... and then re-frozen slowly at −80°C and finally re-thawed at 4°C before determination of protein concentration with a Pierce BCA assay against bovine serum albumin (BSA) as a standard (Thermo Fisher Scientific). Proteins were separated by denaturing SDS-PAGE (10 - 15% (w/v ...
-
bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... The digested peptides were fractionated using a high-pH reversed phase (RP) peptide fractionation kit (Thermo Scientific). Fractions collected from the high-pH RP fractionation were further separated using on-line second-dimension low-pH reversed-phase chromatography via an Easy-nLC 1200 nanoflow-UHPLC system (Thermo Scientific ...
-
bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Peptide concentration was quantified with a plate reader using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific). Finally ...
-
bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
-
bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
-
bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... The peptide concentration in the supernatant was quantified using a quantitative colorimetric peptide assay from Thermo Fisher Scientific (Cat ...
-
bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... with an internal size standard (GeneScan™ 500 LIZ™ dye Size Standard, Applied Biosystems, Waltham, MA) on an ABI3730xl DNA Analyzer (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... and with or without CLASPIN pS945 peptide (MEELLNLC(pS)GKFTSQ) (Peptide Protein Research Ltd, Bishops Waltham, UK) at different concentrations in a Thermoscientific 96 well UV microplate (Fisher scientific, Loughborough, UK). The plates were incubated at 25 °C and absorbance at 340 nm was measured in a POLARstar Omega multimode microplate reader (BMG Labtech GmbH ...
-
bioRxiv - Microbiology 2021Quote: LC-MS/SM analysis of trypsin-digested proteins (peptides) was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by the Sequest software program (Thermo Fisher Scientific, Waltham, MA) 57 ...
-
bioRxiv - Cell Biology 2023Quote: ... Concentrations of proteins and peptides were calculated from the UV absorption at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific, DE; USA).
-
bioRxiv - Cell Biology 2021Quote: ... or an in-house quality control (QC) protein sample was added per well of a 12% polyacrylamide gel along with 8 μl PageRuler Standard (Thermo Fisher, Cat #26616). Samples were prepared such that 2 identical blots were run at the same time ...
-
bioRxiv - Microbiology 2021Quote: Supernatant collected from dentilisin stimulated hPDL cells were quantified and normalized to an albumin standard using the Pierce™ BCA protein assay kit (Cat# 23225, Thermo Scientific, USA) according to the manufacturers protocol ...
-
bioRxiv - Immunology 2021Quote: CHLA255 or KCNR tumor cells expressing green fluorescence protein and luciferase were expanded under standard culture conditions (described above) and harvested with Trypsin (Gibco, Thermo Fisher Scientific). For the orthotopic experiments 1e6 KCNR-GFP Luc or 1e6 CHLA255-GFP Luc tumor cells were implanted into the renal capsule as previously descibed57 ...
-
bioRxiv - Biochemistry 2021Quote: ... SDS-PAGE Bolt™ 4-12% Bis-Tris Plus Gel and PageRuler™ Unstained Protein Ladder molecular weight standard were bought from Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... Protein concentration was determined by the bicinchoninic acid protein assay (Smith et al., 1985) using bovine serum albumin as the standard (Pierce, ThermoFisher, Rockford, IL, USA).
-
bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
-
bioRxiv - Microbiology 2021Quote: ... Labeled peptides of all samples were mixed equally and desalted by peptide desalting spin columns (Thermo Fisher, 89,852). A C18 column (Waters BEH C18 ...
-
bioRxiv - Plant Biology 2022Quote: LC-MS/MS analysis of peptides and phosphopeptides – Peptides mixtures were analyzed by nanoflow Easy-nLC (Thermo Scientific) and Orbitrap hybrid mass spectrometer (Q-exactive HF ...
-
bioRxiv - Systems Biology 2022Quote: ... Peptide concentrations were determined using a fluorimetric peptide assay kit following the manufacturer’s instructions (Thermo Scientific, Cat#23290).
-
bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
-
bioRxiv - Cell Biology 2021Quote: ... Phospho-peptide enrichment was then performed using the High-SelectTM TiO2 Phospho-peptide Enrichment kit (Thermo Fisher Scientific). Each replicate was divided between two TiO2 columns and prepared according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Peptides were then desalted by reverse-phase chromatography using Pierce peptide desalting spin columns (Thermo Fisher part #89852). Final resuspension was done with Solvent A (0.1% formic acid in Fisher Optima LC/MS grade water ...
-
bioRxiv - Plant Biology 2022Quote: ... Peptides were subsequently fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, San Jose, USA) and pooled into 8 fractions [43] ...
-
bioRxiv - Biophysics 2023Quote: ... The concentration of peptide was determined using Pierce quantitative fluorometric peptide assay (Cat# 23290, Thermo Scientific, Rockford, IL). 11 – 18 μL of the peptides were analyzed through nano-ESI-LC/MS/MS using the Orbitrap Exploris mass spectrometer coupled to a Dionex nano-LC system (Thermo Scientific ...
-
bioRxiv - Systems Biology 2023Quote: Phospho-peptide enrichment was carried out using the High-Select TiO2 Phospho-peptide Enrichment kit (Thermo Fisher, A32993), as per manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... The peptide concentration of this pool was determined using a fluorimetric peptide assay kit (Thermo Scientific, Cat#23290). Peptide concentrations per sample were estimated by multiplying the optical density recorded at harvest with the ratio between pool peptide concentration and the median at-harvest optical density.
-
bioRxiv - Cancer Biology 2023Quote: The peptide amount in each sample was estimated using a quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Within each batch ...
-
bioRxiv - Molecular Biology 2023Quote: ... The peptide mix was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific). The 8 obtained fractions were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF ...
-
bioRxiv - Microbiology 2023Quote: ... of 78 of 15-mer peptides covering DBV GnH formulated at 0.625μg/ml for each peptide in RPMI medium (Gibco) in the 96-well plate ...
-
bioRxiv - Cell Biology 2024Quote: ... soluble peptide suspension was transferred into fresh Eppendorf tubes and peptide concentration was determined by microBCA (Thermo Scientific). Equal amounts of peptides for each sample (150-200 µg ...
-
bioRxiv - Genetics 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce Quantitative Peptide Assays kit (ThermoFisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip ...
-
bioRxiv - Pathology 2020Quote: ... a standard curve was generated using OA standard samples with a spectrometer (Evolution™ 201/220, ThermoFisher, USA). Meanwhile ...
-
bioRxiv - Microbiology 2020Quote: ... A standard curve was generated by serially diluting the stock standard curve in DNAse/RNAse free water (Invitrogen) and running the curve concurrently with the samples being tested.